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Citations for Millipore Sigma :
2001 - 2050 of 5830 citations for QuantiChrom Indican Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... LATS1 kinase was immunoprecipitated from 500 µg of protein lysates and subject to Oxyblot assay (#S7150, Millipore) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Time course assay coverslips were incubated with mouse anti-acetylated α-tubulin (1:2000, Sigma Aldrich, T6793) and rabbit anti-ARL13B (1:800 ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged for 15 min at 20 000 g and protein concentration was assessed using Bradford assay (Sigma). 50 µg of protein for each sample was transferred in fresh tubes ...
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bioRxiv - Physiology 2024Quote: ... Immediately prior to the assay the media was changed to glycolytic stress test medium (Sigma Aldrich D5030) supplemented with 143 mM NaCl and 2mM L-glutamine (Gibco 25030 – 081) ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Immunology 2024Quote: ... the culture supernatant was used for cytokine quantification by a 32-plex Luminex assay (Milliplex, Millipore Sigma) in accordance with the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2023Quote: Glutamate and maltose concentrations were determined using enzymatic colorimetric assays purchased from Sigma-Aldrich (St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... and differentiated 3T3-L1 adipocytes (2.5x103 cells/well) in DMEM assay medium composed of DMEM (Sigma #5030) supplemented with 31.6 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The effect of KLF5 knockdown on cell viability/proliferation was measured by the MTT assay (Sigma M2128) at 72hrs (or at the indicated time point ...
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bioRxiv - Immunology 2023Quote: ... Cell concentrations and viability were determined using the ViaCount assay using a Guava easyCyte machine (Millipore-Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... Its effect was measured using the 3-(4,5-dimethylthiazol-2yl)-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cholesterol depletion and supplementation assays were performed in the presence of both 40 μM pravastatin (Sigma-Aldrich) and 50 nM of Shh-N for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were glucose-starved for 60 minutes in seahorse assay base media containing 2 mM glutamine (Sigma). Glycolysis was measured upon glucose injection (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cell concentrations and viability were determined using the ViaCount assay using a Guava easyCyte machine (Millipore-Sigma).
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bioRxiv - Plant Biology 2023Quote: ... and subjected to western blot assay using HRP-conjugated anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: β-Galactosidase assays were done by determining the velocity of hydrolysis of o-nitrophenyl-β-galactoside (ONPG, Sigma) in microtiter plates using a protocol previously described with slight modifications (24 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates for DNA-RNA hybrid pull-down assays were treated with 10 µL RNaseA (Sigma # R6148) or with 10 µL RNaseH (NEB # M0297S ...
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bioRxiv - Biophysics 2023Quote: ... the assay buffer for the standards and the ALP substrate solution (0.04 g phosphatase substrate (Sigma Aldrich) in 10 mL alkaline buffer solution (1.5 M ...
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bioRxiv - Neuroscience 2023Quote: ... Flies used in optogenetic assays were reared on food containing 0.25mM all-trans-retinal (Sigma-AldrichSigna-Aldrich) in darkness ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 20 μl of imaging buffer (assay buffer supplemented with the protocatechuic acid (PCA, Sigma Aldrich)/protocatechuate 3,4-dioxygenase (PCD ...
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bioRxiv - Systems Biology 2023Quote: ... membrane permeability was quantified for each assay by addition of FITC-dextran (4000 MW, Sigma Aldrich 46944) (0.5 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The assay deep-well plate was sealed with an AeraSeal breathable membrane (Sigma-Aldrich, cat. No. A9224) and incubated at 37°C anaerobically for 5 h ...
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bioRxiv - Biophysics 2024Quote: ... malachite green assay (Malachite green oxalate salt, Sigma-Aldrich, cat M6880, Ammonium molybdate tetrahydrate, Sigma, cat 09878) was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... Hepatic triglycerides were measured using the Triglyceride Quantification colorimetric-/fluorometric assay (MAK266, Sigma-Aldrich, St. Louis, US). A hematoxylin and eosin (HE ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay following by the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: The cell death detection kit (In Situ Cell Death Detection Kit, Sigma-Aldrich) was used to perform TUNEL analysis according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Trichrome stain (Masson) kit and senescence cells histochemical staining kit (Sigma-Aldrich, Germany); DAB substrate kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Immunology 2021Quote: ... Arginase activity in the CECs or splenocytes lysates and cell supernatant was determined using Arginase Activity Assay (Sigma) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Plaque assays were undertaken in vero E6 cells using a 1.2% (w/v) microcrystalline cellulose overlay (Sigma-Aldrich). Cytokines/inhibitors were used at the following concentrations ...
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bioRxiv - Cell Biology 2022Quote: Migration assays were performed in transwell polycarbonate membrane cell culture inserts with a 5µm pore (Corning, Sigma-Aldrich) using 1.25×105 hTERT and primary MSCs ...
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bioRxiv - Cell Biology 2019Quote: Bulk kinetic dextran release assays were performed with OG-LUV loaded with fluorescein-dextrans (70 kD)(Sigma; FD70S) or cascade blue (CB)-dextrans (10 kD)(Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Annexin V-FITC apoptosis assay was carried out as described in manufacturer’s protocol (APOAF, Sigma. St. Louis, MO). After transfected with Npac RNAi or control RNAi plasmid in 6-well dishes and selected for 4 days ...
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bioRxiv - Plant Biology 2019Quote: ... The MUG assay buffer is same as that of the extraction buffer supplemented with 1 mM MUG (Sigma). The reaction was incubated at 37°C water bath for 20 min ...
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bioRxiv - Bioengineering 2019Quote: ... GAG quantities were determined using a modified dimethyl methylene blue (DMMB) assay.[72] Shark chondroitin sulfate (Sigma; C4348) was included as a standard ...
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2019Quote: ... The Duolink® in situ proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Then the medium was collected and replaced with stimulation medium (basal assay medium containing 100 µM histamine, Sigma) for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... The assay was developed by addition of o-phenylenediamine dihydrochloride (OPD, Cat n. 34006, Sigma Aldrich, Gillingham, UK) for 10 minutes and reactions were stopped with 2 M H2SO4 ...
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bioRxiv - Bioengineering 2020Quote: ... GAG was quantified using a modified dimethyl methylene blue (DMMB) assay [42] with Shark chondroitin sulfate (Sigma, C4348) as a standard ...
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bioRxiv - Biochemistry 2021Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma-Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Biochemistry 2020Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Plant Biology 2021Quote: ... The stress assays were performed on CM plates (Holliday 1974) supplemented with 750 µM calcufluor white (Sigma-Aldrich), 3 mM hydrogen peroxide (H2O2) ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... and for flippase specific assays in the presence of an ATP regeneration system (0.5 mM Phosphoenolpyruvate (Sigma-Aldrich) and 100 U ml−1 pyruvate kinase (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2021Quote: ... Measurement of IDH activity on the enriched mitochondrial fractions was conducted using an IDH activity assay (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cell pellets were thawed on ice before adding 50-100μl radio immunoprecipitation assay (RIPA) buffer (Sigma, Dorset, UK) with protease and phosphatase inhibitors and lysed for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed beads were resuspended in 25 μl of kinase assay buffer with 100 μM ATP (A7699, Sigma) supplemented with phosphatase inhibitors and incubated for 20 min in an Eppendorf Thermomixer at 30°C at maximal mixing velocity ...