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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Nucleoflagellar apparatuses (NFAps) were obtained by vigorously treating the cells with 1% Igepal CA-630 (Sigma Aldrich) (Sanders and Salisbury ...
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bioRxiv - Pathology 2021Quote: ... The PBS-insoluble SMG mucus was exposed to surfactant (1 mM in PBS, IGEPAL CA-630, Sigma) and solubility was reexamined 48 hours later.
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bioRxiv - Systems Biology 2020Quote: ... 60μL of NADPH solution (2mg of β-NADPH (Sigma-Aldrich; Cat#N7505; CAS: 2646-71-1 (anhydrous)) in 3mL KPE ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed 36 h later in a buffer containing 1% Igepal CA-630 detergent (Sigma, I3021), 150 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... brain slices were incubated with mouse neuronal nuclei antibody (anti-NeuN, 1:500; Millipore, Temecula, CA, USA), rabbit glial fibrillary acidic protein antibody (anti-GFAP,1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... Ten μlitres of 1mg/ml MTT solution (10 mg of MTT (Sigma Aldrich, CAS-298-93-1) in 10 mL of PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM oxidized Glutathione (GSSG, Sigma-Aldrich, CAS-27025-41-8, pH 7.4 in 1 M HEPES) or HEPES were prepared freshly ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM reduced glutathione (GSH, Sigma-Aldrich, CAS-70-18-8, pH 7.4 in 1 M HEPES), 5 mM oxidized Glutathione (GSSG ...
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bioRxiv - Neuroscience 2024Quote: HEK293E cells and MEFs were lysed in RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 200,000 protoplasts were mixed with 50 μg of plasmid DNA and 1 mL of polyethylene glycol solution (40% [w/v] PEG4000 from Sigma-Aldrich, 0.4 M sucrose, 0.1 M CaCl2). After incubation at room temperature for 15 min ...
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bioRxiv - Physiology 2024Quote: ... the supernatant was collected and the islets were lysed in each well using RIPA buffer (50 mM TRIS Euromedex 200923-A, 150 nM NaCl Euromedex 1112-A, 0.5% Sodium deoxycholate Sigma 30970, 1% IGEPAL CA-630 Sigma I8896, 1% SDS Sigma L3771) under agitation during 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 500 μL cold lysis buffer (1X Halt protease inhibitor [Thermo Fisher], 0.5 mg/mL lysozyme, 1:1000 benzonase [EMD Millipore], and 0.1% NP-40 in 1X PBS). Cells were lysed by four 30-second rounds of bead beating at speed 6 with Lysing Matrix B (MP Bio ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 500 μL cold lysis buffer (1X Halt protease inhibitor [Thermo Fisher], 0.5 mg/mL lysozyme, 1:1000 benzonase [EMD Millipore], and 0.1% NP-40 in 1X PBS). Cells were lysed by four 30-second rounds of bead beating at speed 6 with Lysing Matrix B (MP Bio ...
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bioRxiv - Neuroscience 2024Quote: A 1 ml syringe with a 34-gauge needle was used to penetrate the tympanic membrane to inject 100 mg ml-1 sodium arsanilate (A9528 Sigma-Aldrich, 40 μl, prepared in 0.3 M NaHCO3) into the middle ear ...
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bioRxiv - Molecular Biology 2020Quote: ... twice with LiCl wash buffer (10 mM Tris-HCl [pH 8], 250 mM LiCl, 0.5% sodium deoxycholate, 1 mM EDTA, 0.5% IGEPAL CA-630 [Sigma]), and twice with 10 mM Tris-HCl [pH 8] ...
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bioRxiv - Cell Biology 2021Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW: 114,17; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cell Biology 2022Quote: ... A stock solution (1 mg/mL) of T4 (CAS 51-48-9; MW, 776.87 g/mol; Sigma-Aldrich) was prepared by dissolving 1 mg of T4 in 1 mL of DMSO (dimethyl sulfoxide ...
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bioRxiv - Neuroscience 2021Quote: ... and for VGluT2 (1:2000, guinea pig anti-VGluT2 polyclonal antibody, product #AB2251-I, Millipore, Temecula, CA, USA) for 1 hour at room temperature plus 36 hours at 4° C ...
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bioRxiv - Molecular Biology 2021Quote: ... twice with LiCl wash buffer (10 mM Tris-HCl [pH 8], 250 mM LiCl, 0.5% sodium deoxycholate, 1 mM EDTA, 0.5% IGEPAL CA-630 [Sigma]), and twice with 10 mM Tris-HCl [pH 8] ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1% IGEPAL CA-630 in nuclease free H2O) were tumbled with 10 μg anti-m2,2,7G antibody (#MABE302, Sigma) at 4°C for at least 6 hrs ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies were diluted in 1% Tween in PBS or 5% Bovine Serum Albumin (Sigma, CAS # 9048-46-8) prepared in 1% Tween in PBS (filtered ...
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bioRxiv - Cancer Biology 2023Quote: Protein extracts were prepared using RIPA buffer (150 mM NaCl, 1% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0; Millipore Sigma) supplemented with protease inhibitors (cOmplete protease inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... we dissolved 15 mg of low melting agarose (Agarose low gelling temperature, Sigma-Aldrich, CAS# 39346-81-1) in 1 mL of LB medium in a block heater at 70 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was resuspended in buffer C (40 mM HEPES pH 7.6, 4 mM MgCl2, 0.6% Triton X-100, 0.5% IGEPAL CA-630, 20% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Biophysics 2022Quote: ... with 5 % fluoro-surfactant 1H,1H,2H,2H-Perfluoro-1-octanol (CAS Nr 647-42-7, Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... We induced Cas 9 expression by treatment with 1 μg/ml Doxycycline hyclate (DOX, Sigma-Aldrich, D9891-1G) or left the cultures uninduced ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysis was carried out at 4 °C with a 1 mM Tris-HCl buffer (pH 8.0) that included 0.5% IGEPAL CA-630 (Sigma), 0.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... β-tubulin (1:2 000 in 5% skim milk/TBS-T, Cat. No. T8328, Sigma-Aldrich, Oakville, ON, CA). The signals were detected by secondary horseradish peroxidase conjugated antibodies (1:10 000 in 5% skim milk/TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... the cells were activated with a combination of 40 ng/ml PMA (P8139, Sigma Aldrich), 40 ng/ml TNFα (T0157 ...
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bioRxiv - Neuroscience 2021Quote: ... supernatant was replaced with DMEM containing 40 units/mL of DNase type IV (Sigma D5025). Physical cell dissociation was performed by gently flowing microstructures 10 times through a 1 mL micropipette tip ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 U/mL RNase inhibitor) and 0.5 mg of acid washed glass beads (Sigma #G8772) were added ...
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bioRxiv - Immunology 2021Quote: ... minced using fine scissors and incubated for 40 minutes in 1.25mg/ml collagenase D (Sigma), 0.85mg/ml collagenase V (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical tissue was lysed for 45 min on ice in 0.5 % NP-40 (Sigma-Aldrich), 0.5 % SDS ...
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bioRxiv - Cell Biology 2021Quote: ... 40 mM EDTA) and resuspended in 150 µl SP1 buffer + 0.8 mg/mL zymolase (MilliPore), 1 mg/mL lysing enzyme (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 40 µl lysis buffer + β-mercaptoethanol and 0.5 mg/mL 3xFLAG-peptide (Sigma) and incubated 30 minutes at 4°C to dissociate proteins bound to the FLAG-beads ...
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bioRxiv - Biochemistry 2020Quote: The nuclear isoform of Pif1 (amino acids 40-859) was cloned into the pET28a (Novagen) expression vector to add an amino-terminal 6XHis tag ...
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bioRxiv - Neuroscience 2020Quote: ... Formazan crystals formed were solubilized in a buffer containing 40 % (v/v) dimethyl-formamide (Sigma), 10 % (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 40 min with Phalloidin TRITC (2.5 µg/ml) (P1951, Sigma, USA) and mounted using Prolong Glass Antifade Mountant with NucBlue stain and incubated for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 40 min with Phalloidin TRITC (2.5 μg/ml) (P1951, Sigma, USA) to counter stain samples and mounted using Prolong Glass Antifade Mountant with NucBlue stain and incubated for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.3% NP-40 (lysis buffer) supplemented with 3μL phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 1μg/mL each of leupeptin ...
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bioRxiv - Cell Biology 2021Quote: ... pH ~7.4) and permeabilized for 10 min in PEM + 0.1% NP-40 Alternative (Millipore, 492016) + 6.6 nM Alexa Fluor™ 488 Phalloidin (and 0.2x Tubulin Tracker Deep Red (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... and treated with 40 mM methyl-β-cyclodextrin (Sigma C4555; 5-10 mins on ice) to sequester fatty acids ...
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bioRxiv - Immunology 2022Quote: ... the pellet was carefully lysed in 50 µl resuspension buffer supplemented with NP-40 (Sigma), Tween-20 and Digitonin (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... 40 μg/ml glucose catalase and 14 mM MEA in H2O (all reagents from Sigma).
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bioRxiv - Biophysics 2022Quote: ... Clarified supernatant was incubated with 40 μL anti-FLAG M2 affinity gel (Sigma-Aldrich A2220) for 3 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... cells were chemically lysed by resuspension to OD600 = 1.0 in 40 μL Bug Buster (Novagen) for 5 min at 25° C with intermittent vortex ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were reduced with 60 µL 40 mM dithiothreitol (DTT; Sigma-Aldrich, Taufkirchen, Germany) in 50 mM TEAB ...
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Developmental effects of oxytocin neurons on social affiliation and processing of social informationbioRxiv - Neuroscience 2021Quote: ... larvae (∼30-40 per petri dish) were treated with metronidazole (MTZ, Vetranal CAT#46461, Sigma) dissolved in Danieau buffer to a final concentration of 10mM for 48 h while being protected from light ...