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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: PC12 and RSC96 cell/scaffold constructs were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma) and phalloidin-Alexa Fluor 594/488 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated for 5 min with a 4’,6-diamidino-2-phenylindole (DAPI) solution (Sigma) to stain cell nuclei ...
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bioRxiv - Immunology 2023Quote: ... and/or 4 μM N,N,N’,N’-tetrakis-(2-pyridyl-methyl)-ethylenediamine (TPEN; Sigma, #P4413) was added to the cells ...
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bioRxiv - Bioengineering 2023Quote: ... nuclear staining was done with 0.1 μg/ml DAPI (4′,6-diamidino-2-phenylindole) (Sigma-Aldrich) for 1 minute ...
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bioRxiv - Microbiology 2023Quote: ... The AF food contained 1.25 mM captan (N-trichloromethylmercapto-4-cyclohexene-l,2-dicarboximide; Sigma-Aldrich) with coconut oil (300 μL/ L standard media ...
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bioRxiv - Microbiology 2023Quote: ... followed by PBS washing and incubation with DAPI (4’,6-diamidino-2-phenylindole dihydrochloride [Sigma-Aldrich]) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... ovaries were counterstained with DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma-Aldrich, India; 1µg/ml), washed thrice in PBST for 20 minutes each ...
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bioRxiv - Neuroscience 2023Quote: ... PBS containing 50 ng/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich, cat#D8417) was used for counter nuclear staining ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were visualized with DAPI (4′,6′-diamidino-2-phenylindole)-containing Fluoroshield mounting medium (Sigma-Aldrich). A Nikon A1RMPsi laser scanning confocal microscope was used for imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI, Sigma; diluted 1ug/ml in PBS) for 3 min at RT and coverslips were mounted with Prolong Diamond Mounting Media (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with fluorescently labelled secondary antibodies and reacted with 4′,6-diamidino-2-phenylindole (DAPI, Sigma) before mounting ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 0.001mg/mL in 1x PBS, Sigma-Aldrich) for 5 min in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The samples were fixed overnight at 4°C in the mixture of 4% paraformaldehyde (Cat. No. 162-16065, Wako Pure Chemical Industries) and 2% glutaraldehyde (Sigma-Aldrich, G5882) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 h at 4°C before being rotated overnight at 4°C with Anti-acetyl-Histone H3 (Lys27) (Sigma; 07-360) or Anti-MAFG (GeneTex ...
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bioRxiv - Genomics 2024Quote: ... slides were counterstained in 4’,6-diamidino-2-phenylindole (DAPI) solution and embedded in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: ... HB10Y samples were titered prior to freezing by serially diluting samples in 10 millimolar (mM) of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma PN#H4034-100G) + 10% Sucrose (Sigma PN #S7903-250G) ...
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bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set, and Sigma phosphatase inhibitor set), incubated on ice for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... HB10Y samples were titered prior to freezing by serially diluting samples in 10 millimolar (mM) of 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES, Sigma PN#H4034-100G) + 10% Sucrose (Sigma PN #S7903-250G) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2023Quote: ... The endocarp was submerged in 1:4:5 v/v/v mixture of 30% hydrogen peroxide (HX0635-2; EMD Millipore, Burlington, MA, USA), distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Cell Biology 2023Quote: Cells were arrested in prometaphase by incubating them for 2 – 4 h in 200 ng ml-1 nocodazole (Sigma-Aldrich, 31430-18-9). Mitotic exit was induced by adding flavopiridol (Tocris Bioscience ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM MgOAc, 1 mM CaCl2, 200 mM sorbitol, 2 mg/mL iodoacetamide, and protease inhibitors [Pierce A32965 or Sigma 11836153001]). Resuspended pellets were dripped slowly into liquid nitrogen to form “popcorn” and were stored at −70°C until further processing ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mg aliquots of homogenized tissue were incubated with 1 ml CTAB buffer (2% w/v) + 10 µl 2-mercaptoethanol (Sigma M3148) at 55°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 µg 14:0 Lyso PE and 0.4 µg (14:0)2-PS in a final volume of 90 µl of chloroform/methanol 2/1 (Sigma Aldrich, France). Lipids were extracted according to the method of Folch with 4410 µl of chloroform/methanol 2/1 for 1 h followed by 1250 µl of saline for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then centrifuged at 10,000×g for 2 minutes and incubated with 1 mL of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich 93443) for 30 minutes with gentle agitation ...
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bioRxiv - Biophysics 2021Quote: ... The cantilevers were functionalized with amine groups by immersing in 2% (vol/vol) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Bioengineering 2021Quote: ... was added to the PEG solution followed by the addition of 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate] (HATU; 2.43 g, 6.4 mmol, 2 eq.; Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: ... 2- or 3-days post fertilisation (dpf) zebrafish were anaesthetised in 0.168 mg/ml Tricaine (Sigma-Aldrich) in E3 media and visualised under a dissecting microscope ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, MO) as described previously.52 Equivalent MTT assays were performed on cells cultured in the same ratios of PBS to media ...
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bioRxiv - Immunology 2021Quote: ... or 25 µM (L-3-trans-(Propylcarbamyl) oxirane-2-carbonyl)-L-isoleucyl-L-proline (CA-074me) (Sigma) to inhibit cathepsin B ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Cancer Biology 2020Quote: ... collected and lysed with NP-40 lysis buffer supplemented with phosphatase inhibitor cocktails 2 and 3 (Sigma) and protease inhibitor mix (Sigma) ...