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Citations for Millipore Sigma :
1951 - 2000 of 3767 citations for Somatoliberin GHRH Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or isotype-control human IgG Ab (10 μg/ml; Sigma-Aldrich) was incubated on de-paraffinized tissue sections overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... fibrinogen (final conc. of 7.35 mg/mL, from human plasma, Sigma), thrombin (from human plasma ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were coated with either 1x Fibronectin human plasma (Sigma) or poly-D-lysine (20 µg/mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with FN (Sigma-Aldrich, Darmstadt, Germany, human plasma) and 5x105 cells were seeded and allowed to attach 5-10 min ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
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bioRxiv - Neuroscience 2024Quote: ... Human cell line samples: mouse anti-β-Actin (Sigma-Aldrich, A1978); rabbit anti-PARP (Cell Signalling Technology ...
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bioRxiv - Immunology 2024Quote: Human umbilical vein endothelial cells (HUVECs, #CRL-1730™, Sigma-Aldrich) were maintained in Endothelial Cell Growth Medium (#211-500 ...
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bioRxiv - Biochemistry 2024Quote: ... and human CNS tissues were extracted with RIPA lysis buffer (Millipore) containing the protease/phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: 16HBE14o- (human bronchial airway epithelial cells) were purchased from EMD Millipore Corporation (Temecula ...
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and FGF (Fibroblast growth factor basic human, Sigma-Aldrich, Cat#SRP6159) at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All hTERT-HME1-derived cells lines were maintained in DMEM/F12 medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2mM L-glutamine (Pan-Biotech ...
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bioRxiv - Immunology 2020Quote: Cell lines were grown at 37°C in either RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich, UK), 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... At 1 h post infection (hpi) the infection medium was exchanged with DMEM/10% FCS containing 100 µg/ml gentamicin (GEN, Sigma-Aldrich G1397) and increasing concentrations of CIP (0 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed brain samples were placed securely one at a time in a custom-made MR-compatible holder and immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, UK). Samples were scanned in a random order ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS; PAA, Cölbe, Germany) and 50 U/ml penicillin plus 5 μg/ml streptomycin (Sigma-Aldrich, Munich, Germany). Peripheral blood mononuclear cell (PBMCs ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL 5% FC-40 oil (RAN Biotechnologies, CN 008-FluoroSurfactant-5wtF) was added to the bottom and 50 µL mineral oil (Sigma, CN M5904) was added on the top ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 % fetal calf serum (FCS) as well as Pen/Strep (50 U/ml penicillin + 50 μg/ml streptomycin; Sigma-Aldrich, USA). After centrifugation at 900 g ...
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bioRxiv - Microbiology 2024Quote: ... the infected cells were washed twice and further incubated in DMEM/10% FCS containing 100 μg/ml gentamicin (GEN, Sigma-Aldrich G1397) to remove extracellular bacteria ...
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bioRxiv - Immunology 2022Quote: ... and transferred in the digestion medium (HBSS with Ca2+ Mg2+ - Fetal Calf serum (FCS) 2% - DNase I 0.1mg/mL (Sigma-Aldrich, ref 11284932001) – Collagenase IV 0.5mg/mL (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Immunology 2023Quote: ... CD44-FUT7-Fc collected from cell culture medium was concentrated 200-fold using Amicon® Ultra-15 centrifugal filter units (Millipore Sigma), and 10µl of this concentrate was added to block the HUVEC substrate for 1h before performing the rolling assay at 1dyn/cm2 ...
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bioRxiv - Immunology 2023Quote: ... 110-HG) in the presence or absence of 1 μg mCD152/Fc (CTLA4-Ig, mouse IgG2a, non-cytolytic) chimera (Sigma-Aldrich, C4358). The anti-human IgG Fc Antibody (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MB49 was cultured in RPMI media (Biochrom GmbH) supplemented with 10% FCS (Biochrom GmbH) and 50 μg/ml Gentamycin (Sigma, Darmstadt, Germany).
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bioRxiv - Cell Biology 2024Quote: ... were thawed and washed with Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with 5% foetal calf serum (FCS) once before incubating with 1mg/ml collagenase P (Sigma Aldrich, UK) and 100μg/ml DNase I (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO). Microglia were isolated using anti-Cd11b beads (130-049-601 ...
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bioRxiv - Cancer Biology 2019Quote: ... Gartner’s medium consisted of alpha-MEM (BioWhittaker) supplemented with 12.5% heat-inactivated FCS and 12.5% heat-inactivated Horse serum (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in an incubator at 37°C and 5% CO2 with 100% humidity in a 1:1 mixture of EGM-2 with M199 (Thermo Fischer, 31150022) supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich, F7524-500ML), 20 µg/ml gentamicin (Thermo Fischer ...
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bioRxiv - Cancer Biology 2021Quote: ... Human primary fibroblasts (HFFF2) were treated with 50μM of Etoposide (Sigma-Aldrich) for 2 days and cells collected at day 7 or treated with 1μM Palbociclib for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted once each using human IgY14 LC10 (Sigma S5074) and human Supermix LC5 (Sigma S5324 ...
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bioRxiv - Cell Biology 2020Quote: All the substrates were functionalized with human plasma fibronectin (FN, Sigma-Aldrich). After sterilizing with UV for 30 min ...
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bioRxiv - Immunology 2021Quote: ... and 10% human serum (heat-inactivated, sterile-filtered, male AB plasma; SIGMA). Human IL-2 (TECIN™ teceleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human islets were washed in 1x PBS and resuspended in Accutase (Sigma) dissociation solution for 3 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with rabbit anti-human SOX9 primary antibody (AB5535, Millipore) in the blocking solution for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... for Mouse 448 and human experiments or 1 mg/ml Hoechst (Sigma) for the mouse atlas experiments ...
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bioRxiv - Biophysics 2022Quote: ... consisting of OF-medium complemented with 10μg/ml human transferrin (Sigma-Aldrich), 20nM human insulin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Immunology 2022Quote: Human dermal fibroblasts at passage 20 were cultured in complete DMEM (Sigma) supplemented with 1% pen/step (Sigma ...
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bioRxiv - Genomics 2020Quote: ... Purified rabbit polyclonal antibody against human TCF21 (HPA013189) was purchased from Sigma. Recombinant human TGFβ (AF-100-21C ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 7.5 IU human chorionic gonadotropin (Cat.# CG5-1VL, Sigma-Aldrich) 46-48 h later ...