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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Recombinant Mouse Icam2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin and mouse anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA); mouse anti-His (CoWin Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Either mouse M2 Flag or mouse HA-7 primary antibodies (Sigma-Aldrich, St. Louis, MO) were used at a 1:1000 dilution in 2.5% BSA in TBST for 1 hour at room temperature ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... mouse monoclonal anti-β actin and mouse monoclonal anti-FLAG (clone M2) were from Sigma; rat monoclonal anti-CD40 (3/23) ...
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bioRxiv - Neuroscience 2023Quote: ... pre-coated with mouse anti-GFP (A11120, Life Technology) or mouse anti-Flag (F3165, Sigma) using Dynabeads Antibody Coupling Kit (14311D ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse, H9658 Sigma-Aldrich, 1:1000) and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-FLAG (Anti-FLAG M2 mouse monoclonal antibody, F1804-1 Sigma-Aldrich, 1:1000), mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:1000 anti-HA mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich), or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Cancer Biology 2020Quote: ... All hTERT-HME1-derived cells lines were maintained in DMEM/F12 medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2mM L-glutamine (Pan-Biotech ...
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bioRxiv - Immunology 2020Quote: Cell lines were grown at 37°C in either RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich, UK), 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Microbiology 2022Quote: ... At 1 h post infection (hpi) the infection medium was exchanged with DMEM/10% FCS containing 100 µg/ml gentamicin (GEN, Sigma-Aldrich G1397) and increasing concentrations of CIP (0 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed brain samples were placed securely one at a time in a custom-made MR-compatible holder and immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, UK). Samples were scanned in a random order ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS; PAA, Cölbe, Germany) and 50 U/ml penicillin plus 5 μg/ml streptomycin (Sigma-Aldrich, Munich, Germany). Peripheral blood mononuclear cell (PBMCs ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL 5% FC-40 oil (RAN Biotechnologies, CN 008-FluoroSurfactant-5wtF) was added to the bottom and 50 µL mineral oil (Sigma, CN M5904) was added on the top ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 % fetal calf serum (FCS) as well as Pen/Strep (50 U/ml penicillin + 50 μg/ml streptomycin; Sigma-Aldrich, USA). After centrifugation at 900 g ...
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bioRxiv - Immunology 2022Quote: ... and transferred in the digestion medium (HBSS with Ca2+ Mg2+ - Fetal Calf serum (FCS) 2% - DNase I 0.1mg/mL (Sigma-Aldrich, ref 11284932001) – Collagenase IV 0.5mg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... CD44-FUT7-Fc collected from cell culture medium was concentrated 200-fold using Amicon® Ultra-15 centrifugal filter units (Millipore Sigma), and 10µl of this concentrate was added to block the HUVEC substrate for 1h before performing the rolling assay at 1dyn/cm2 ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Microbiology 2024Quote: ... the infected cells were washed twice and further incubated in DMEM/10% FCS containing 100 μg/ml gentamicin (GEN, Sigma-Aldrich G1397) to remove extracellular bacteria ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...