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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... protein G agrarose (60 μl, Millipore) was incubated with anti-phosphotyrosine antibodies (12 μg 4G10 (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... catalase (40,000 units/mg protein, SIGMA) was added to the reaction mixture to deplete the resulting hydrogen peroxide ...
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bioRxiv - Cell Biology 2020Quote: ... nonstructural protein 9 (NSP9, Sigma, SAB3701435), FLAG (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... protein A-HRP (Millipore, Billerica, MA), goat anti-mouse-HRP (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with ECL (Millipore) using a Pxie machine.
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bioRxiv - Genetics 2020Quote: ... Proteins were digested with trypsin (Sigma) overnight at 37°C ...
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bioRxiv - Genetics 2020Quote: ... and 10% protein (BSA (Sigma A1470), HSA (Sigma A8763) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein G beads (Sigma).
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bioRxiv - Physiology 2023Quote: ... protein concentration was quantified by Novagen BSA assay (71285-3 ...
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bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase conjugated Protein G (Sigma) was used as 2ndaly antibody ...
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bioRxiv - Microbiology 2024Quote: ... Protein A- agarose beads (Millipore Sigma) were washed 3 times with 1 × immunoprecipitation (IP ...
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bioRxiv - Developmental Biology 2024Quote: ... prewashed Protein G agarose beads (Millipore) were added to the protein antibody mix and incubated at 4°C for 4 hours on an orbital shaker ...
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bioRxiv - Cell Biology 2023Quote: ... and Apo-1 protein sequence (Sigma) along with Proteome Discoverer™ contaminant database ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with DTT (Sigma) and alkylated with iodoacetamide (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested with trypsin (Sigma) overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A Sepharose® beads (Sigma) were coated with PBS + 0.3% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... protein A Sepharose (Sigma-Aldrich #P3391) was added to the mixture and incubated for 4 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and Protein A Sepharose (Sigma-Aldrich), and purified mAbs were buffer-exchanged into phosphate buffered saline (PBS).
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hyaluronic acid binding protein (Millipore) was used at 10 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... or protein A-agarose beads (Sigma) for 2-3 hours at 4℃ ...
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bioRxiv - Developmental Biology 2024Quote: ... EZView protein G affinity beads (Sigma) were washed with lysis buffer A 3x times ...
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bioRxiv - Neuroscience 2024Quote: ... protein kinase C (Sigma-Aldrich; P5704), ACSL4 (Abgent ...
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bioRxiv - Developmental Biology 2024Quote: ... EZView protein G affinity beads (Sigma) were washed with lysis buffer A 3x ...
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bioRxiv - Molecular Biology 2024Quote: ... protein inhibitor cocktail (539134, Millipore Sigma), sodium fluoride ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein extraction with BugBuster (Novagen), the target tag-SLO protein underwent Ni2+-IMAC resin (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... protein were combined with a mixture of 50 % (v/v) protein A and 50 % (v/v) protein G beads (Sigma-Aldrich Inc., Cat#P9424 and Cat#P3296) and 0.5-1 μg antibody in lysis buffer overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 60 ng of recombinant HA-BACH1 in 21 μl of a reaction buffer containing 10 μg of bovine ubiquitin (Sigma-Aldrich), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... stable PDA cell lines were generated by infecting 70% confluent cultures with the recombinant lentiviral vectors with 10 mg/mL polybrene (TR-1003, Sigma-Aldrich) twice over 2 consecutive days ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore, Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).