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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Male mESC cells were resuspended in differentiation medium containing 15% FBS (Millipore, Cat. No.ES-009-B), 1X Glutamax (Thermo Fisher ...
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bioRxiv - Physiology 2019Quote: ... Cell culture antibiotics (concentrations used are taken from reference (Karp et al., 2002): amphotericin B (Sigma); gentamicin (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... Small batches of liposomes were remotely loaded with 11 moles percent Amphotericin B (AmB, Sigma A4888) relative to 100% liposomal lipid to make AmBisome-like AmB-LLs used throughout this study ...
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bioRxiv - Pathology 2020Quote: ... and 4-methylumbelliferyl N-acetyl b-D-glucosaminide (Sigma Aldrich, Cat. No. M2133, St. Louis, MO).
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bioRxiv - Physiology 2020Quote: ... 50 mM imidazole) and solution B (solution A plus 5 mM ouabain (Sigma-Aldrich, O3125-1G)) were prepared ...
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bioRxiv - Biochemistry 2021Quote: Ferrioxamine B (DFO) and the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) were purchased from Sigma-Aldrich, 55FeCl3 was purchased from Perkin Elmer ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Westerns blots were performed and normalized against b-actin (1:10,000, MABT825, Millipore, Billerica, MA, USA) as previously reported by our group [25] ...
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bioRxiv - Biophysics 2021Quote: Fluorescent cell walls were obtained by metabolic insertion of Rhodamine B (Sigma-Aldrich, St. Louis, USA). The Rhodamine dye was added to the culture medium at a concentration of 1μM ...
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bioRxiv - Genomics 2021Quote: ... 15 g/L agar) supplemented for mutant strains with hygromycin B (Sigma-Aldrich; 70 μg/ml) or nourseothricin (Werner Bioagents ...
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bioRxiv - Immunology 2021Quote: ... B Bedogni (University of Miami, FL) and were cultured in DMEM supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (HyClone) ...
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bioRxiv - Immunology 2020Quote: ... and 0.25 μg/mL of amphotericin B per mL (complete medium - all reagents from Sigma-Aldrich). To separate the trypomastigote forms from the culture ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 6 ml buffer B containing 15 mM d-desthiobiotin (Sigma Aldrich). Purification of GyrA-N-Strep and GyrB-C-Strep were conducted in the same way using an adjusted buffer Bgyr for cell disruption and purification (buffer Bgyr ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content in each well was evaluated by the SRB assay (Sulphorhodamine B, SIGMA, cat. S1402) as described elsewhere98.
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bioRxiv - Immunology 2022Quote: ... Strains expressing GFP were grown in media supplemented with 50 μg/mL hygromycin B (Sigma-Aldrich), and strains expressing luxABCDE were grown in media supplemented with 20 μg/mL Zeocin (Thermo).
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bioRxiv - Microbiology 2022Quote: Adherent neutrophils (not IL-8 treated) were exposed to 5 µg mL−1 cytochalasin B (Sigma) for 20 min followed by 1 µM fMLF (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates are obtained by sonication and CelLytic™ B Cell Lysis Reagent (Sigma-Aldrich, US). Cell debris are removed by centrifugation at 24,000 g and crude Rubiscos were obtained by ammonium sulfate precipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... B cell activation was achieved with by exposing cells to with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... Solution B is composed of 14.3 mM of each of the twenty amino acids (Sigma, USA), 236.3 mM phosphoenolpyruvate (PEP) ...
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bioRxiv - Microbiology 2023Quote: ... sclerotiorum KO and KI mutants were screened and purified on PDA supplemented with hygromycin B (Sigma) at a final concentration of 50 μg/ml.
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/amphotericin B (Sigma, St. Louis, MO). Cells were transfected using TransIT-LT1 Transfection Reagent (Mirus Bio ...
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bioRxiv - Microbiology 2023Quote: ... 1 mg of Cyt B was dissolved into 100 μl of dimethyl sulfoxide (DMSO; Sigma-Aldrich) and 15 μl aliquots of the resulting Cyt B solution were added to each reactor ...
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bioRxiv - Cell Biology 2023Quote: ... 300 U/mL streptomycin sulphate (#A1852 AppliChem) and 7.5 µg/mL amphotericin B (#A2942 Sigma-Aldrich). Excess tissue ...
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bioRxiv - Molecular Biology 2023Quote: ... and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml amphotericin B (Sigma)) at 37°C in a moist atmosphere containing 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... These flasks were treated with a 0.1% gelatin solution in water (Millipore Sigma, ES-006-B) for 30 minutes prior to plating cells ...
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bioRxiv - Immunology 2022Quote: ... CD20+IgG+ or CD20+IgD+IgM+CD27-IgG- B cells were sorted into FBS (Sigma-Aldrich) using a FACSAria Fusion cell sorter (Becton Dickinson) ...
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bioRxiv - Molecular Biology 2023Quote: ... media was changed for media containing 5,6-dichlorobenzimida-zole 1-b-D-ribofuranoside (DRB; Sigma, D1916) at a final concentration of 50 μM ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 250 µl primary B cell media supplemented with 5 µg/ml LPS (Sigma).
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bioRxiv - Microbiology 2023Quote: ... 20 mM NaHCO3) supplemented with 10% (v/v) CelLytic™ B cell Lysis Reagent (Sigma-Aldrich) and 0.1% Protease Inhibitor Cocktail (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Two control groups were used: (a) Distilled water and (b) ivermectin (5 mg/mL, Sigma-Aldrich). A ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing H2B-mRuby were then enriched using selection with 0.5 mg/ml hygromycin b (Sigma) and verified using fluorescence microscopy and flow cytometry (LSRFortessa flow cytometer ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were treated with 1 μg/mL doxycycline and 50 μM biotin (Sigma-Aldrich # B-4639) for 24 hours for BirA* fusions or 30 minutes for miniTurbo fusions ...
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bioRxiv - Neuroscience 2024Quote: ... gently dounce-homogenized (KIMBLE Dounce tissue grinder, Sigma D8938, 6 strokes with A and B pestle), and incubated for 5 minutes in a total of 4 ml EZ buffer.
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bioRxiv - Neuroscience 2024Quote: ... Mass spectrometer calibration was performed with a [Glu1]-fibrinopeptide B human (Glu-Fib) (Sigma-Aldrich, USA) solution with an MS/MS spectrum reference from the NanoLock Spray source ...
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bioRxiv - Microbiology 2021Quote: Serum concentrations of Metabolic Panel (Ghrelin, Glucagon, Insulin and Leptin) were determined using the Mouse Metabolic Magnetic Bead Panel Milliplex MAP kit (Millipore).
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bioRxiv - Microbiology 2021Quote: ... IgE) and IgA in feces supernatant were determined using the Mouse Immunoglobulin Isotyping Magnetic Bead Panel Milliplex MAP kit (Millipore) and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Cell Biology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink® In Situ Red Mouse/Rabbit kit (DUO92008, Sigma-Aldrich, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting plasma supernatant was transferred to a sterile Eppendorf tube and stored at −80 °C until the cytokines were measured using a Milliplex xMAP Mouse High Sensitivity TCell Magnetic Bead Panel kit MHSTCMAG-70K (Millipore) following the kit protocol on a Luminex 200 system with Xponent (v2.7) ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... The PLA was performed using reagents supplied in the Duolink in situ Red PLA Mouse/Rabbit kit (Sigma-Aldrich, DUO92101) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The Millipore Mouse Cytokine 32-plex kit was used to measure the serum concentration of 32 cytokines (Cat# MCYTMAG-70K-PX32, Millipore). xPONENT (Luminex ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Glycaemia was measured using a glucometer and insulin levels using the Milliplex Map Kit (Mouse Metabolic Magnetic Bead Panel. MMHMAG-44K, Millipore) Milliplex Map Kit ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Ear homogenate supernatants were assayed for chemokines and cytokines with the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel kit (Millipore). Samples were collected and analyzed using BioPlex 200 reader (BioRad).
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...