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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 30% WNT conditioned medium (home-made) and 10μm Rho-kinase inhibitor (Y27632, Sigma-Aldrich), for the first week ...
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bioRxiv - Cell Biology 2022Quote: ... then the reactions were incubated in kinase buffer with 20 μM ATPγS (Sigma-Aldrich) at 30°C for 45 min ...
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bioRxiv - Biochemistry 2019Quote: Casein phosphorylation reactions were performed with 300ng kinase and 25µM dephosphorylated alpha casein (Sigma) in PMP buffer plus 100μM cold ATP ...
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bioRxiv - Neuroscience 2019Quote: To block cell proliferation the cyclin-dependant kinase (CDK) inhibitors Olomoucine and Ryuvidine (Sigma) where applied ...
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bioRxiv - Neuroscience 2020Quote: ... Src-kinase inhibitor (2 µM SU 6656, S9692 Sigma-Aldrich, St. Louis, MO, USA) was added ...
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bioRxiv - Synthetic Biology 2023Quote: A two-enzyme cascade comprising Cytophaga PPK2 and rabbit creatine kinase (CK) (Sigma-Aldrich) was applied to sequentially convert the algal polyP into creatine phosphate via ATP ...
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bioRxiv - Neuroscience 2023Quote: CKý activity was measured with the creatine kinase activity assay kit from Sigma-Aldrich according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were: rabbit anti-Barhl1 polyclonal antibody (Atlas Antibodies, HPA004809, Sigma, 1:500), goat anti-Robo3 polyclonal antibody (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... (4) rabbit anti-Sox9 was used at 1:2000 (AB5535; Millipore). This antibody was raised against human synthetic peptide amino acids VPSIPQTHSPQWEQPVYTQLTRP ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-CD9 (C-4, Santa Cruz; C9993 Sigma; Novus NBP2-67310), (anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Immunology 2024Quote: ... blots were probed overnight at 4°C with anti-LC3 (Sigma) and anti-actin (Cell Signalling ...
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bioRxiv - Cell Biology 2020Quote: ... and beta-actin (catalog #A5441) (Sigma-Aldrich, St. Louis, MO, USA; dilution 1:10,000). Signals were revealed using peroxidase-conjugated secondary antibodies ...
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bioRxiv - Pathology 2019Quote: ... sc-21733 – Santa Cruz Biotechnology) or beta-actin as endogenous control (1:500, B1305554-40TST, SG100806AD, Sigma Aldrich). On the next day ...
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bioRxiv - Cell Biology 2021Quote: ... and beta-actin (1:10,000; A5441) were purchased from Sigma Aldrich (Sant Luis, USA). Secondary antibodies coupled to horseradish peroxidase used for immunoblotting were obtained from Sigma (A4914 Goat anti-Rabbit IgG).
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bioRxiv - Cancer Biology 2020Quote: ... so animals were administered with 8 μg/ml 17-beta estradiol (Sigma-Aldrich, #E2758) in drinking water at least 4 days previously to implantation until the end of the experiment ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.1 mM beta-mercaptoethanol and 106 units/L leukaemia inhibitory factor (LIF) (ESGRO, Millipore) and grown on a six well plate until 70% confluent ...
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Metformin protects trabecular meshwork against oxidative injury via activating integrin/ROCK signalsbioRxiv - Pharmacology and Toxicology 2022Quote: ... For drug testing, tBHP (Damas-beta, China) and metformin (Sigma-Aldrich, St. Louis, USA) were dissolved in DMEM/F12 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected Kuramochi control cells were then treated with TGF-beta (10 ng/ml; Sigma) for 2 hours before measurement to serve as a positive control for activated NF-κB activity ...
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bioRxiv - Biophysics 2023Quote: ... cells were treated with 10 mM methyl-beta cyclodextrin (MbCD; Sigma-Aldrich, Darmstadt, Germany) in serum-free DMEM after three washing steps with DPBS+/+ to obtain a control sample featuring an increase in membrane fluidity ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) powder (Millipore Sigma, D1916-50MG) was dissolved in DMSO for a final stock concentration of 100mM ...
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bioRxiv - Bioengineering 2019Quote: ... cells were permeabilized via 0.02% Tween 20 for 20 minutes and incubated overnight at 4°C with rabbit anti-SLCO1B3 (OATP1B3) primary antibody (2-μg/mL working concentration, HPA004943, Sigma-Aldrich Canada, Oakville, Ontario, Canada). Goat anti-rabbit AlexaFluor 647-conjugated secondary antibody was then applied for two hours at room temperature (1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with antibodies against selected proteins overnight at 4°C: Anti-β-Tubulin III (β-Tub III) (Sigma, T8660, mouse, 1:2000); anti-p-Tau (Cell Signaling ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4 °C in the following primary antibodies diluted in TBST: 1:200 dilution of anti-CRABP2 (MAB5488, EMD Millipore/Sigma Aldrich, St. Louis MO), 1:200 dilution of anti-RALDH2 (#13951-1-AP ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3x with 1x PBS and incubated with appropriate primary antibody (Table 4) diluted in 4% BSA (Sigma, A9418-50G) in 1x PBS overnight at 4C ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at room temperature followed by incubation overnight at 4°C with indicated antibodies NFIB (HPA003956, rabbit antibody, 1:1000 dilution, Sigma); AR (ab74272 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody is diluted in blocking buffer and slides are incubated in primary antibody overnight at 4°C (ChAT, Millipore, AB144P ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were incubated overnight at 4°C in the presence of primary antibodies in PBS-T: rabbit PYK2 antibody (1/500, Sigma #07M4755 ...
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bioRxiv - Neuroscience 2021Quote: Antibodies and dilutions were as follows: Anti-HNK-1 antibody (Sigma Aldrich, Monoclonal Anti-HNK-1/N-CAM (CD57), # C6680) ...
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bioRxiv - Neuroscience 2019Quote: ... PV (mouse anti-Parvalbumin monoclonal antibody, 1:3000, Swant) and SOM (rat-anti Somatostatin monoclonal antibody 1:250, Millipore). The following corresponding secondary antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were incubated with a monoclonal anti-myc antibody (Covance, MMS-150R) or anti-Flag antibody (Sigma, F3165) for 2 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The magnetic beads were then conjugated with anti-FLAG antibodies by adding 1 µg anti-FLAG antibody (Sigma, #F3165), followed by a 30-minute incubation at RT with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse 1:10,000 or anti-rabbit 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were placed back in fresh complete medium containing propidium iodide (PI) (5 µg/ml, Sigma) for an additional hour in a regular cell culture incubator (20% O2 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA content of the cells was determined by propidium iodide (PI) staining (40 μg/mL; Sigma), and progression of the cell cycle monitored by fluorescence-mediated counting (FACSCalibur ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS followed by staining with 10μg/ml of PI (Sigma-Aldrich (USA)) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 mM polyP-45 (average chain length 45 Pi units; Sigma-Aldrich, Oakville, ON, Canada) in Ham’s F12 medium for 30 minutes or medium alone (control) ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were then stained with propidium iodide (PI) (LifeTechnologies) and with PNA-fluorescein isothiocyanate (FITC)(Sigma), both at a final concentration of 1 µg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by staining with propidium iodide (PI) staining solution (1:500 dilution) (Sigma-Aldrich) at 37°C for 30 min and Annexin V-FITC (1:1500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... propidium iodide (PI) and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were bought from Sigma Aldrich (Shanghai). Ala and antifungal drugs were dissolved in DMSO to get stock solutions of 20 mM.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed and suspended in Sort buffer containing propidium iodide (PI, 0.5μM, Millipore Sigma, # P4170), 7-AAD (1μg·mL-1 ...
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bioRxiv - Cell Biology 2022Quote: ... neutrophils were treated with phosphatidylinositol-specific phospholipase C (PI-PLC, 0.5 units/mL, Sigma-Aldrich, P5542) at 37°C for 30 minutes with gentle stirring as previously described22.
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bioRxiv - Immunology 2019Quote: ... CD14 peridinin-chlorophyll protein complex (PerCP, clone TM1, DRFZ Berlin) and propidium iodide (PI, Sigma Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Bis-benzamide H 33342 trihydrochloride (Hoechst) and propidium iodide (PI) from Sigma (St. Louis, MO, USA). MitoTracker Green ...
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bioRxiv - Physiology 2022Quote: ... The crude nuclear suspension was spiked with 6 μL of propidium iodide (PI, Sigma, 1mg/mL) to label all nuclei ...