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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for 7 Chloro 1 heptene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Genetics 2021Quote: ... were resuspended in 4 volumes lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM dithiothreitol, 1 mM ethylenediaminetetraacetic acid, 1:400 Sigma protease inhibitory cocktail P8340 ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Cell Biology 2023Quote: ... and lysis buffer (1% Triton-X, 10 nM NEM, 1 mM PMSF, 1 x Protease Inhibitor Cocktail, Sigma-Aldrich) was added ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Biochemistry 2023Quote: ... 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000, anti-UBR4/p600 ab86738, Abcam, 1:5000, anti-FLAG M2, Sigma F1804 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Neuroscience 2023Quote: ... CaBP (1:2000, rabbit, CB38, Swant or 1:2000, mouse, #300) and VGLUT2 (1:7000, guinea pig, AB2251, Millipore). The slices were washed 3 times in 1% Triton X-100 in PBS for 5 to 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... or PV antibody (1:500, Cell Signaling D3S8R; SST: 1:100, Millipore MA5-16987; PV: 1:1000, Swant GP72) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% ITS-X supplement (WAKO) and purified BSA (3%, WAKO)) and N2B27 (DMEM/F12/GlutaMax + Neurobasal at 1 : 1 (Sigma), 1 × B27 (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were incubated with the following antibodies against phospho-Yap at s112 (1:1000, 13008, cell signaling), Yap (1:2000, NB110-58358, Novus) and Rabbit anti GAPDH (1:8000, PLA0125, Sigma) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... five flies of each sex and genotype were placed into a vial with 2 ml of 1 X dyed medium (2.5 % yeast, 2.5 % sucrose, 1 % agar and 1 % Brilliant Blue FCF – Sigma Aldrich, USA). After 2 h of feeding ...
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bioRxiv - Neuroscience 2019Quote: ... for the final differentiation in neural differentiating medium (NDM) (Neurobasal 1% NEAA, 1% N2 supplement and 1% P/S) supplemented with 1µM of cAMP (Sigma-Aldrich) and 10 ng/mL of BDNF ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in 5% horse serum in 0.5% Triton-X TBS for 1 hour before overnight incubation with 1:500 anti-GFP or 48 hours 1:300 anti-UBE3A (3E5 Sigma) and 1 hr 1:500 Alexa-Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251]; rabbit anti-TH 1:1000 [Millipore #AB152] ...
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bioRxiv - Biochemistry 2020Quote: ... The full-length MtaLonA were prepared by supplementing the growing medium with 1 g l−1 of 15NH4Cl and 4 g l−1 of 2H7/12C6-glucose in 99.8%-2H2O (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... anti-γH2AX (JBW301, 1:1000), anti-P-Ser15-p53 (1:1000) and anti-ATM Ser1981 (10H11.E12, 1:200) were from Millipore. Anti-P-KAP1Ser824 (1:1000) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then with Theobroma cacao extract fraction 5 (100 µg.mL-1) or (-)-epicatechin (80 µg.mL-1) (Sigma-Aldrich, HW101708-1) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS/0.1% Triton X-100 and incubated with the secondary antibody (1:500) and 1 mg ml−1 DAPI (Sigma, D9564) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 12565501) with 0.01M phosphate-buffered saline and processed immediately for zonula occludins-1 (ZO-1; 1:500, Millipore Sigma, MABT11) and Bmal1 (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and transfer vector at 1:1:1 molar ratio using the X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich; # 6365787001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: Transfected COS-7 cells were washed three times with ice cold PBS and scraped in buffer K (1 mM Tris-HCl, 1 mM EGTA and 1 mM MgCl2, pH 7.6) containing protease inhibitor cocktail (Sigma, # P2714). Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Genomics 2020Quote: ... The blocking solution was aspirated and replaced with the same solution with primary antibodies (MAP2-Ck, Abcam ab5392, 1:500; Nanog-Gt, R&D AF1997, 1:200; TRA-1-60 IgM-Ms, Millipore MAB4360 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Cancer Biology 2019Quote: ... This wash step was repeated 3 times and the chloroform fraction was finally dried befor e being redissolved in 200 ml of 50% methanol prior to be loaded into a conditioned (1 CV methanol, 1 CV MiliQ water, 1 CV acetonitrile (Sigma) and 1 CV Mili-Q Water ...
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bioRxiv - Molecular Biology 2019Quote: ... and SmB(residues 1-91) [pCDFDuet-HT-B(1-91)-D3(1-75)] were constructed in a single pCDFDuet vector (Novagen) with N-terminal His(6)-tag followed by Tobacco Etch Virus (TEV ...
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bioRxiv - Genomics 2021Quote: ... The beads were washed two times with high-salt wash (50 mM Tris-HCl, pH 7.4, 1 M NaCl, 1 mM EDTA, 1% Igepal CA-630 (Sigma I8896), 0.1% SDS ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...