Labshake search
Citations for Millipore Sigma :
1951 - 2000 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The culture supernatant containing the target protein was concentrated 3-fold and buffer exchanged against 6 volumes of working buffer (50 mM Tris/HCl, 300 mM NaCl, pH 8.0) by crossflow filtration (Millipore Pellicon 2 mini filter ...
-
bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
-
bioRxiv - Immunology 2021Quote: ... Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The stained tissue sections were scanned using an Olympus VS-120 slide scanner and imaged using a Hamamatsu ORCA-R2 C10600 digital camera for all bright-field and fluorescent images ...
-
bioRxiv - Neuroscience 2020Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
-
bioRxiv - Biochemistry 2021Quote: ... EMSA reactions were then run on a non-denaturing 0.5X TBE 6% polyacrylamide gel and stained with ethidium bromide (Sigma). Band intensities were quantified using ImageJ.
-
bioRxiv - Cell Biology 2021Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
-
bioRxiv - Cell Biology 2020Quote: ... and a thrombin solution containing CaCl2 and aprotinin (final concentration of fibrin components: 6 mg/mL fibrinogen (Sigma-Aldrich); 2 NIH U/mL thrombin from human plasma (Sigma-Aldrich) ...
-
bioRxiv - Plant Biology 2020Quote: ... and pre-cultivated on MS1 medium[MS0 with 0.1 g/L IAA (Sangon Biotech Co., Ltd, Shanghai, China) and 1.5 g/L 6-BA (Sigma, USA)] ...
-
bioRxiv - Microbiology 2020Quote: ... and naïve CD4+ T cells (6 × 104 cells/well) were plated together with 0.1 nM retinoic acid (Sigma-Aldrich) and 2 ng/ml recombinant mouse TGF-β (R&D Systems) ...
-
bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...
-
bioRxiv - Cancer Biology 2020Quote: ... MD2G (3 μg) and pLKO-LV-gene-specific (6 μg) plasmids in the presence of PEI transfection reagent (Sigma). The generated lentiviral particles were collected 24 hours post-transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
-
bioRxiv - Cell Biology 2020Quote: ... The fixed animals were then washed twice with PBSTx1 and stained with 1μg/mL DAPI (4’,6-diamidino-2-phenylindole)(Sigma) solution for 30 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...
-
bioRxiv - Genetics 2022Quote: ... one million cells were seeded in 6-well plates and co-transfected with 1μg of total DNA using the ESCORT IV transfection reagent (Sigma) following the instructions of the manufacturer ...
-
bioRxiv - Genetics 2022Quote: ... Three days later 200 μl of the transfected cells were seeded into new 6-well plates treated with 0.01% poly-L-Lysine (Sigma), followed by selection with 1mg/ml of Zeocin (Invitrogen) ...
-
bioRxiv - Molecular Biology 2022Quote: ... filtered through 0.45 mm filters and concentrated to 6 mL in Amicon Ultra 100K centrifugal filter units (Millipore Sigma). Concentrated lentiviral particles were used immediately or stored at -80°C.
-
bioRxiv - Neuroscience 2022Quote: ... The skull was exposed and a burr hole was drilled to introduce a syringe for a single unilateral MFB injection of either vehicle (0.2mg/ml ascorbic acid in 0.9% sterile saline; pH5.0) or 6-OHDA (hydrobromide salt (Sigma Chemicals, UK); 3μg/μl in vehicle) ...
-
bioRxiv - Neuroscience 2022Quote: ... a total of 60 male (eight-week-old) C57BL/6 mice were treated with streptozotocin (STZ, Sigma-Aldrich, USA) injection at a dose of 50 mg/kg for 5 consecutive days ...
-
bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
-
bioRxiv - Developmental Biology 2022Quote: ... cells were then spun down (2 min with 1000 rpm) and resuspended in 6 ml serum-free medium (Sigma Cat# ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of 200,000- 300,000 cells per well in a 6-well plate in the presence of Polybrene transfection reagent (Sigma- Aldrich) and infected with 10% of the total virus from one 10 cm plate of the indicated constructs ...
-
bioRxiv - Bioengineering 2022Quote: ... then stained with 4’,6-amidino-2-phenylindole (DAPI) and mounted with polyvinyl alcohol with DABCO (PVA-DABCO; Sigma). A list of antibodies and concentrations used for IF can be found in Supplementary Table 4 ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were transfected with 6 μg of pCG1_SARS-2_S using X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich). Twenty hours later ...
-
bioRxiv - Molecular Biology 2020Quote: ... MEFs were plated in 6- well plates (10,000 per well) and treated continuously with different doses of PARPi or cisplatin (Sigma) for 7 days ...
-
bioRxiv - Molecular Biology 2020Quote: ... coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich) and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS ...
-
bioRxiv - Neuroscience 2021Quote: ... and plated on sterile 6 or 24-well plates coated with poly-D-Lysine (sterile lyophilized 135 kD; Sigma) and 1mg/ml laminin ...
-
bioRxiv - Neuroscience 2021Quote: ... They were then administered three consecutives intraperitoneal (i.p.) injections (6 mg/kg body weight) of the glutamate agonist kainic acid (KA) (Sigma) dissolved in 0.1 M PBS pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... the monolayers were fixed with 10% (v/v) neutral buffered formalin for at least 6 h (NBF, Sigma-Aldrich) and stained with 0.2% (v/v ...
-
bioRxiv - Biochemistry 2020Quote: ... fixed and stained for β-galactosidase activity at pH = 6 using Senescence Cells Histochemical Staining Kit (Sigma, Cat#CS0030). For rescue assays ...
-
bioRxiv - Neuroscience 2021Quote: ... Switzerland) were dissolved in glacial acetic acid brought to pH 6 with the addition of sodium hydroxide (NaOH, Sigma). Haloperidol (1mg/kg) ...
-
bioRxiv - Biochemistry 2021Quote: ... were mixed with equal volume of 6 M guanidine hydrochloride solution and desalted by using ZipTip C18 (Millipore, USA). The protein was extensively washed with 0.2% (v/v ...
-
bioRxiv - Biochemistry 2020Quote: ... the plates were incubated for 6 days at 37°C and were then stained with 0.2% (w/v) crystal violet (Sigma) in 2% ethanol for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... and seeded at a density of 2 × 106 cells per well (6-well plates) or 0.5 × 106 cells per well (24-well plates) into PDL/Laminin (Sigma)-coated plates with neuronal medium plus 0.5 μM RA ...
-
bioRxiv - Neuroscience 2022Quote: ... HeLa cells or iPSC-derived motor neurons grown on 6-well plates were rapidly rinsed with ice-cold PBS and subjected to ATP extraction with 0.5% TCA (Sigma). After incubation for 15 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: Immunofluorescence (IF) was performed in tissue sections (6-8mm) blocked with 10% horse serum and 0.3% TritonX-100 (Sigma) for 1 hour at room temperature (rt) ...
-
bioRxiv - Developmental Biology 2022Quote: ... were obtained from previous studies (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747754/) cultured in E14 plus complete medium (Glasgow Minimum Essential medium(Sigma-Aldrich G5154-6) supplemented with 10% heat inactivated FBS (Fisher Scientific SH3007103) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were infected with the lentiviruses in the presence of 6 μg/mL polybrene (Sigma-Aldrich, Cat. # H9268) for 24 h.
-
bioRxiv - Developmental Biology 2022Quote: ... serum-LIF-grown ESCs were split with accutase onto laminin-coated 6-well/plates (Sigma, 10μg/ml in PBS) at a density of 0.75×104 cells/well in plain N2B27 medium ...
-
bioRxiv - Bioengineering 2022Quote: ... and a medium with an extracellular signal-regulated kinase inhibitor (ERKi, MilliPore Sigma #1049738-54-6, Burlington, MA, USA), adapted from Eigler et al.2 More details on the media used are provided in Supplementary Table 1.
-
bioRxiv - Biochemistry 2019Quote: HEK293T shHuR/shAUF1 and shControl cells (9.6 × 105 cells) in 6-well plates were treated with 5 μg/mL actinomycin D (Sigma) and harvested in Trizol as described above at the indicated time points ...
-
bioRxiv - Neuroscience 2019Quote: ... the sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI, 100 ng/ml in PBS, Sigma, cat: D9542) for 10 minutes ...
-
bioRxiv - Plant Biology 2019Quote: ... From the working solution an assay buffer was made adding 50U of Glucose-6-Phosphate Dehydrogenase (G6PDH; Sigma-Aldrich), and 295µL or 280µL of the working solution was added to a 96 well plate (Costar ...
-
bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
-
bioRxiv - Developmental Biology 2020Quote: ... We added potassium phosphate buffer pH 6 to the samples to a final concentration of 0.1 M and trehalase enzyme (SIGMA) to 1.5 U/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...