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Citations for Millipore Sigma :
151 - 200 of 696 citations for VSIR Cynomolgus HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells were plated on poly-L-Lysine-coated (Sigma; P4707) 10 cm plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B and HEK-293T cells (ATCC) were grown in DMEM (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: HEK 293T cells were cultured in DMEM with 10% FBS (Millipore Sigma), GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... HCC5044 and HEK-293T cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... TPC-1 and HEK 293T/17 were cultured in DMEM (Sigma D6429) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... typically in an equal mixture of Expi293 and EX-CELL 293 (14571C Sigma). Expi293 media can be used for the entire process ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% heat inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T cells were grown in in Dulbecco’s Modified Eagle Medium (DMEM, Sigma) with the addition of 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2019Quote: HEK 293T cells were transiently transfected using X-treme gene 9 (Sigma Aldrich). After 48 hours of the transfection ...
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bioRxiv - Biophysics 2019Quote: ... All HEK cell culture media were supplemented with 10% foetal calf serum (Sigma) and 1% Pen/Strep (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibodies were transiently expressed in HEK 293A cells following polyethylenimine (Sigma-Aldrich) transfection (Sherbenou et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK 293T cells were transfected with Cdt1 expression plasmids using PEI Max (Sigma) and cultured for 16 hours ...
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bioRxiv - Cell Biology 2022Quote: ... HEK cell culture medium contained Dulbecco’s modified eagle medium (D6046, Sigma-Aldrich, Germany). Both media were supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 293T HEK and MEF in Dulbecco’s modified Eagle’s medium (Sigma Aldrich, D6046-500ML). The culture media was supplemented with 10% foetal bovine serum (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... the cells were exposed to HEK media with 1 µg/mL doxycycline (Sigma) to induce expression of the BFP/iCasp9 from the landing pad ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were transfected with pLVX-FAP-TRF1 and Mission Packaging Mix (Sigma) to produce lentivirus ...
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bioRxiv - Biophysics 2019Quote: ... AAVs were generated in HEK 293T cells and purified by iodixanol (Optiprep, Sigma; D1556) step gradients as described (Challis et al. ...
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bioRxiv - Genomics 2021Quote: ... Both hPBMCs and HEK 293T cells were cryopreserved in FBS + 10% DMSO (Sigma-Aldrich). Prior to FACS sorting ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: ... 1 x 105 HEK 293T cells/well were seeded onto poly-lysine (Sigma; 7405) coated 24-well plates ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK-3.x cells were detached from the flask using trypsin/EDTA (Sigma-Aldrich) and resuspended in 10 mL Leibovitz’s L-15 media supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2022Quote: Human HEK-293T cells transfected with either LacZ shRNA (control) or IKKα shRNAs (Sigma) along with their corresponding packaging plasmids were used to prepare lentiviruses from cell culture medium 48 h after transfection ...
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bioRxiv - Microbiology 2023Quote: The total RNA from Ace2-HEK-293T cells was isolated using TRI Reagent (Sigma) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...