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Citations for Millipore Sigma :
151 - 200 of 4665 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Immunology 2020Quote: ... 180 uL of co-culture media (RPMI-1640 media containing 1% FBS, LPS (100 ng/mL; from E coli, Sigma-Aldrich) and IFNɣ (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... in the central-left abdomen area and 10 μg of LPS (from E. coli, serotype 055:B5 and deproteinized; Sigma-Aldrich) in 100 μl of PBS in the centralright abdomen area ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10/50 μg/mL Lipopolysaccharide (LPS; E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10 or 50 μg/mL lipopolysaccharide (LPS) (E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Systems Biology 2023Quote: ... All E. coli strains (a gift from Dr. Christian Rudolph) were routinely grown in modified Luria Broth (LB) (1% tryptone (Sigma-Aldrich), 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... either fresh or thawed DCs (differentiation day 8) were stimulated for 24 h with 200 ng/ml lipopolysaccharide (LPS; E. coli O26:B6, Sigma-Aldrich) and used for experiments on day 9 ...
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bioRxiv - Immunology 2023Quote: ... CD11c+ were purified and either plated with no further supplementation or stimulated with 100 ng/mL or 1 μg/mL of lipopolysaccharide (LPS, E. coli O111:B4, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female Cx3cr1-GFP mice bred on a C57BL/6J background (n=2 females and 1 male/condition) were intraperitoneally injected with 0.5 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female C57BL/6J mice (n=2/sex/condition) were intraperitoneally injected once with 1.0 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution 1xPBS (Phosphate buffered saline ...
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bioRxiv - Genetics 2023Quote: ... each probe set was added (final concentration of 500 nM) to 20mM VRC and hybridization buffer (1% Dextran sulfate [EMD Millipore], 1 mg/mL E. coli tRNA [Sigma] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 1 mg/ml AAG (human a1-acid glycoprotein, Sigma, Cat # G9885). All medium is sterilized by filtration through a 0.22mm membrane ...
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bioRxiv - Bioengineering 2021Quote: ... and N,N,N′,N′-tetra-methylethylenediamine (TEMED, Sigma T9281) were used for microwell PA gel polymerization used in both scWB and scIEF ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.05% N,N,N’,N’-tetramethylenediamine (TEMED) (Sigma, T7024) in 2x SSC ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µl of N,N,N ′,N′ - tetramethylethylenediamine (Sigma). The solution was vortexed ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% (v/v) N,N,N’,N’-Tetramethylethylenediamine (Sigma-Aldrich), and PBS ...
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bioRxiv - Genomics 2023Quote: ... N,N,N’,N’-tetramethylethylenediamine (Millipore-Sigma, T7024-25ML, USA), 2.5 µl] ...
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bioRxiv - Genomics 2023Quote: ... N,N,N’,N’-tetramethylethylenediamine (Millipore-Sigma, T7024-25ML, USA), 2.5 µl] ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2% N,N,N’,N’-tetramethylethylenediamine (TEMED) (T7024; Sigma) and sandwiched by a coverslip functionalized by GelSlick (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and TEMED (N,N,N’,N’-tetramethylethane-1,2-diamine, Sigma). The polyacrylamide solution is immediately pipetted onto the chlorosilaned slide and covered by the functionalized coverslip ...
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bioRxiv - Cancer Biology 2023Quote: ... and TEMED (N,N,N’,N’-tetramethylethylenediamine, Sigma, T7024-25ML). The gel solution was then removed ...
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.05% of N,N,N,N’-tetramethylethylenediamine (TEMED, Sigma Aldrich, #T9281), 0.7% of FluoSpheres carboxylate modified microspheres (0.2µm ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.5 μL N,N,N”,N”-Tetramethylethylenediamine (TEMED; Sigma 411019). We cast each gel base with 45 μL and each lid with 35 μL solution ...
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bioRxiv - Cell Biology 2022Quote: ... We then added N,N,N′,N′-tetramethylethylenediamine (TEMED, Sigma-Aldrich) and 10% APS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Coronavirus OC43 (ATCC VR-1558) was propagated in HCT-8 cells and detected by antibody staining (MAB9012, Millipore).
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were placed at −80 °C for long-term storage or in RNAlater (Sigma). Formalin-fixed tissue samples were paraffin-embedded by the Pathology Core at the Centre for Modeling Human Disease (CMHD ...
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bioRxiv - Microbiology 2020Quote: ... Anti-ICP4 MAb H1A021 or monoclonal Ab to VSV glycoprotein G (P5D4, Sigma) was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Then 1 µg/well of biotinylated α1-acid glycoprotein (Sigma, Catalogue number-112150) or 3’-sialyllactose-PAA-biotinylated (Glycotech ...
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bioRxiv - Developmental Biology 2021Quote: ... N,N,N’,N’-tetrakis(2-hydroxypropyl)ethylenediamine (Sigma, 5% (w/w)) ...
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bioRxiv - Biophysics 2020Quote: ... and catalyst N,N,N′,N′-tetramethylethylenediamine (0.5 μL - TEMED, Sigma Aldrich) was used to initiate the reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... 75g of N,N,N’,N’-Tetrakis(2-Hydroxypropyl)ethylenediamine (Sigma, 122262) and 42ml of Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... N,N,N′,N′-Tetramethylethylenediamine (Roth) and 4-Hydroxy-TEMPO (Sigma-Aldrich) to MS ...
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bioRxiv - Cell Biology 2021Quote: ... 25 wt% N,N,N’,N’-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma, 122262) and 15 wt% Triton X-100 (AppliChem ...
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bioRxiv - Neuroscience 2021Quote: ... 25 wt% N,N,N′,N′-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma-Aldrich) and 15 wt% Triton X-100 (Nacalai Tesque) ...
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bioRxiv - Bioengineering 2022Quote: ... and N,N,N’,N’-tetramethylenediamine (0.05% v/v, TEMED, T9281, Sigma) and briefly mixed by vortexing ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.75 µL N,N,N’,N’-tetramethylethylenediamine (TEMED,T7024 Sigma-Aldrich) was added ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 v/v% N,N,N’,N’-tetramethyl ethylenediamine (Sigma Aldrich) was prepared and added to the existing gel layer ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 v/v% N,N,N’,N’-tetramethyl ethylenediamine (Sigma Aldrich) was prepared and casted into 1.00 mm mini-protean glass plates (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... N,N,N′,N′-tetramethyl- p-phenylenediamine (300 µM, # T7394; Sigma-Aldrich) and potassium cyanide (200 μM ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Cell Biology 2019Quote: ... Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA, Sigma) (4.5 mM ...
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bioRxiv - Bioengineering 2020Quote: ... 25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl) ethylenediamine (Sigma Aldrich, 585714), and 5 wt% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... with 100 μM N,N,N’,N’-tetramethyl-ρ-phenylene diamine (Sigma-Aldrich). Glycolytic and mitochondrial ATP production rates were determined according to the XF Real-Time ATP Rate Assay Kit protocol (Agilent) ...
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bioRxiv - Bioengineering 2023Quote: ... 25 wt% N,N,N′,N′-tetrakis (2-hydroxypropyl)ethylenediamine (Sigma-Aldrich, 122262) and15 wt% Triton X-100 in 0.01 M PBS ...