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Citations for Millipore Sigma :
151 - 200 of 4507 citations for Recombinant Human NEK7 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 5 μg/mL recombinant human insulin (Sigma-Aldrich, UK), 0.5 ng/mL recombinant human basic fibroblast growth factor ...
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bioRxiv - Cell Biology 2024Quote: ... 350 μL insulin human recombinant (Sigma-Aldritch, Cat. 11376497001)) supplemented with bFGF (10 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Microbiology 2023Quote: ... GST (Sigma), eGFP (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged SMOCtail and IFT38 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... and brain-derived neurotrophic factor (BDNF, human recombinant, Sigma-Aldrich). Concentrations used were in the range 50-100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37° C for 3 days in a humidified CO2 incubator.
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bioRxiv - Biophysics 2020Quote: ... 100 nM recombinant human EGF (Sigma-Aldrich, St. Luis, MS). For LatA treatments 1 µM LatA was added to cells and incubated for 10 min at 37°C prior to imaging ...
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bioRxiv - Immunology 2019Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator.
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bioRxiv - Physiology 2020Quote: ... 5 μg/mL recombinant human insulin (Sigma-Aldrich, Dorset, UK), 0.5 ng/mL recombinant human basic fibroblast growth factor ...
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bioRxiv - Immunology 2020Quote: ... 300 U/mL Roche recombinant human IL-2 (Sigma-Aldrich) and 1 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Immunology 2020Quote: ... and recombinant human RAP (Merck Millipore, 200 nM final concentration) were used to stimulate nuclear translocation of MerTK.
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml human recombinant epidermal growth factor (Sigma, UK) and 25 μg/ml fetuin from FBS (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng/ml of recombinant human bFGF (F0291, Sigma-Aldrich, 50 μg/mL stock solution in 20 mM Tris pH=7.5 + BSA 0.1 % ...
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL recombinant human insulin (Sigma Aldrich #I9278-5ml), and 1% penicillin-streptomycin (Thermo Scientific #15140122) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg/mL human recombinant insulin (Sigma-Aldrich, 91077C).
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
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bioRxiv - Cell Biology 2024Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
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bioRxiv - Biophysics 2024Quote: ... using either recombinant human EGF (Sigma-Aldrich, St. Louis, MO) or Ephrin A1 as ligand ...
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bioRxiv - Biophysics 2024Quote: ... using either recombinant human EGF (Sigma-Aldrich, St. Louis, MO) or NRG1 as ligand ...
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bioRxiv - Molecular Biology 2024Quote: Human recombinant IFNα A protein (Sigma-Aldrich catalog number: IF007) was diluted in 0.1% BSA and added to the cells for 24 hours before further analysis.
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected with siRNA oligonucleotides from Sigma (600 nM NEK6 600 or 100 nM NEK7) using Dharmafect transfection reagent (Dharmacon ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA for SBP-tagged human FKBP12.6 was subcloned into the pET28 vector (Novagen) containing a 6×His-tag at the N-terminus ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2024Quote: AM 810–813 plasmids (Table S2) encoding active and proteolytically dead (PD) GST-tagged AtATG4A and AtATG4B were used to transform Rosetta (DE3) cells (Novagen, 70954-3). Expression was induced overnight at 16°C using 0.25 mM IPTG (Hellobio ...
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bioRxiv - Molecular Biology 2021Quote: ... After washing Laemmli buffer 1X was added followed by boiling and loading on SDS-PAGE for visualization by western blotting using anti-His (Clonetech 631212) and anti-GST (Sigma G1160) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... The protein was separated on a 10% SDS-PAGE gel and further analyzed by immunoblotting using anti-His and anti-GST antibody (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... GST (Sigma; G1160) and AC3 (LifeSpan BioSciences ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human epidermal growth factor (20 ng/mL, Sigma-Aldrich). Cell authentication by Ipatimup’s Cell Lines Bank ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblast Growth Factor basic human recombinant animal-free (GF003AF; Sigma Aldrich); hEGF Epidermal Growth Factor Human (E9644 ...