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Citations for Millipore Sigma :
151 - 200 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10 μg/ml human insulin (Sigma Aldrich), 10 ng/ml EGF (Peprotech) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10 μg/ml human insulin (Sigma Aldrich), 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Genomics 2019Quote: ... 10 μg/mL human insulin (Sigma Aldrich Cat. 19278), 20 ng/mL hEGF (Gibco Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg/ml human insulin (Sigma-Aldrich, I9278). At day 4 of differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg/ml human insulin (Sigma-Aldrich, cat.no. I9278), 0.5 µg/ml hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... additionally containing 2.5 μg/mL human insulin (Sigma-Aldrich), 1X N2 Supplement (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Insulin concentrations after the 16 h hyperinsulinemia treatment were determined using human insulin RIA kit (Millipore). To mimic starvation ...
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bioRxiv - Biophysics 2021Quote: PAI-1 (human recombinant, Sigma-Aldrich, Germany)
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bioRxiv - Biophysics 2021Quote: ... PAI-1 (human recombinant, Sigma-Aldrich, Germany), Plasminogen (human plasma purified protein ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human thrombin (1 U/ml, Sigma) was added to the bottom of a 3.0 µm costar polycarbonate transwell membrane insert (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... sativa–derived recombinant human albumin (Sigma-Aldrich), and 213μg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10μM recombinant human Gastrin (Sigma-Aldrich G9145), 50ng/mL recombinant human HGF (Peprotech 100-39H) ...
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bioRxiv - Developmental Biology 2019Quote: ... 10.7 μg/mL recombinant human transferrin (Sigma), and 20μg/mL recombinant human insulin (Peprotech) ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant human TNFα (30 ng/mL, Sigma), and C1q (400 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Immunology 2020Quote: ... 0.5% recombinant human serum albumin (A9986, Sigma), 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant 2N4R human Tau (Tau441, AG960, Millipore) at serial dilutions ranging from 20 µg/ml to 0 µg/ml total tau was used to generate a calibration curve and calculate total tau in our samples.
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bioRxiv - Cell Biology 2022Quote: ... 0.025-μg·ml-1 recombinant human EGF (Sigma), 0.1-μg·ml-1 cholera toxin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL human recombinant bFGF (Sigma), and 10 μM Y-27632 (STEMCELL Technologies)) ...
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bioRxiv - Microbiology 2024Quote: ... 5 ng of recombinant human AChE (Sigma) or 30 μg of tissue lysate was incubated with steroidal alkaloid compound or at room temperature for 30 minutes in 50 mM Tris pH 8.0 in a total volume of 90 μL ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant human IFN-α2a (H6041; Sigma-Aldrich) was reconstituted in PBS with 0.1% BSA and added to the basolateral pole at a final concentration of 100 U/ml ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 10−3 g/L human insulin (Sigma Aldrich, USA), 5 × 10−5 M hydrocortisone (Upjohn Laboratories SERB ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 µg/mL human insulin (Sigma-Aldrich®, 15500).
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bioRxiv - Bioengineering 2022Quote: ... 15 μl human Insulin (0.5 μM, Sigma-Aldrich, I2643-50MG), 10 μl T3 (2 nM ...
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bioRxiv - Bioengineering 2021Quote: ... 15 μl human Insulin (0.5 μM, Sigma-Aldrich, I2643-50MG), 10 μl T3 (2 nM ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 μg/ml human insulin (Sigma-Aldrich, St. Louis, MO), 100 μM ß-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... and/or 0.4μg human GST-tagged PP2A-α (Sigma, SRP5336) in the presence or absence of AZ’5576 or okadaic acid (Abcam ab120375 ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2022Quote: ... and 0.5% recombinant human serum albumin (Sigma Aldrich). Microfluidic droplets were generated as water-in-oil emulsions using a co-flow of aqueous phases ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1000U recombinant human LIF (300-05, Sigma) and the small molecules 1μM PD0325901 (13034 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.5 mg/ml human recombinant albumin (Sigma-Aldrich) and 0.2 mg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... 0.5 mg/ml human recombinant albumin (Sigma-Aldrich) and 0.2 mg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant human BDNF and ANA-12 (Sigma-Aldrich) was incubated during 24h at 1 nM and 50 μM ...
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bioRxiv - Cell Biology 2022Quote: ... 10 nM recombinant human [Leu15]-Gastrin I (Sigma), 50 ng/mL mouse EGF (R&D Systems ...