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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminal peptide enrichment was performed on the digested sample by removing the internal tryptic peptides with undecanal (Sigma, U2202). Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Microbiology 2024Quote: ... pETN was generated by restriction cloning using EcoRI and HindIII to clone a codon-optimized version of the N-terminal portion of the INP gene from Pseudomonas syringae into pET22b (Novagen). This INPN gene fragment was obtained as a gBlock (IDT) ...
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bioRxiv - Microbiology 2024Quote: The DNA sequence encoding for PsrSp130–420 and a N-terminal MHis6ENLYFQ-tag was cloned into the pET28a expression vector (Novagen). The tag-PsrSp construct was transformed into the BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and cytochrome C type III (97% purity, Sigma-Aldrich, Inc, Cat. No C-2506; Lot 45F-7195). These proteins were prepared by dilution in 10 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... with some modifications: O-glycans were released from porcine gastric mucin (Sigma Type III, 10% w/v) by incubation at 48°C for 20 hrs in 150 mM NaOH with 750 mM NaBH4 ...
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bioRxiv - Microbiology 2023Quote: ... we added 0.4% or 0.6% (wt/vol) mucin (type III mucin from porcine stomach; Sigma-Aldrich M1778) to BHI powder before mixing with Milli-Q water ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cell Biology 2021Quote: ... ALP activity was determined by ELISA kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... mouse FGF21 ELISA kit (Sigma-Aldrich, EZRMFGF21-26K).
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were harvested using collagenase type IV (Cat N° 10780004) and collected in Corning® ultra-low attachment flasks (Sigma, Cat N° 734-4140) to facilitate embryoid body formation ...
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bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
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bioRxiv - Neuroscience 2023Quote: ... the human nasal epithelium was embedded in 3% Type I-A agarose (Sigma) prepared in ACSF once the agar had cooled to 38°C ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 ng of human urokinase-type PLG activator (uPA, Sigma-Aldrich) was added per well ...
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Microbiology 2022Quote: ... ubiquitin carboxyl-terminal esterase L1 (UCHL1) (29) using rabbit anti-human UCHL1 (Sigma, HPA005993 at 1:1,000 dilution). The secondary antibody was Alexa Fluor 594-conjugated goat anti-rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Neuroscience 2024Quote: ... which was produced using a mouse antibody against the N-terminal extracellular domain of the GluA2 subunit (MAB397, Sigma-Aldrich, USA) and SiteClick™ Antibody Labeling Kits (S10453 ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Biochemistry 2022Quote: ... Fetuin (from fetal bovine serum) and Type III porcine gastric mucin were from Sigma (St. Louis, MI, USA). Lewis antigens (Lea triose ...
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bioRxiv - Physiology 2022Quote: ... frozen liver tissue (30–50 mg) and dilutions of glycogen type III obtained from rabbit liver (Sigma-Aldrich) were homogenized in 0.03 N HCl ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Physiology 2021Quote: We used ELISA kits to detect the concentrations of inflammatory mediators such as TNF-α (KIT ELISA TNF-a-RAB0477-1KT, Sigma-Aldrich) and IL-6 (KIT ELISA IL-6-RAB0308-1KT ...
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bioRxiv - Molecular Biology 2021Quote: ... The levels of Aβ40 and Aβ42 were quantified by sandwich ELISA using Aβ40 and Aβ42 EZbrain ELISA kit (EMD Millipore) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: FGF21 ELISA was performed following the manufacturer’s guidelines (FGF21 ELISA kit, Millipore Sigma catalogue number-EZRMFGF21-26K).
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bioRxiv - Physiology 2022Quote: ... total adiponectin by ELISA kit EZMADP-60K (EMD Millipore), high molecular weight (HMW ...
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bioRxiv - Physiology 2020Quote: ... Insulin was measured in plasma by ELISA kit (Millipore). For IP-GTT (2 g/kg ...
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bioRxiv - Physiology 2023Quote: ... mouse GLP-1 ELISA kit (EZGLP1T) from Sigma-Aldrich; mouse GDF15 ELISA kit (DY6385 ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Pathology 2021Quote: Cell culture inserts were coated with human placental type IV collagen (Sigma-Aldrich, C7521). CRC expanded cells were seeded at 2.5 × 105 cells on 12-mm Snapwells (Corning Costar ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
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bioRxiv - Immunology 2023Quote: ... and an N-terminal NusA fusion separated by a TEV cleavage in a pET-50b (+) was transformed into tuner (DE3) (Novagen; cat # 70623), BL21(DE3 ...