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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Recombinant human insulin (0.75 IU insulin/kg BW) (Sigma-Aldrich, I9278) was then administered intraperitoneally ...
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bioRxiv - Immunology 2019Quote: ... and recombinant human interleukin-2 (rhIL-2; 25U/ml; Sigma-Aldrich). To mimic a transient stimulation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... baseline blood glucose was taken before recombinant human insulin (Sigma, USA) (0.5 U/kg – 1 U/kg ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-3 (SRP7783) was purchased from Sigma Aldrich (Germany).
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml recombinant human epidermal growth factor (hEGF, #E9644, Sigma), 0.25 ng/mL hydrocortisone (#H0888 ...
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bioRxiv - Bioengineering 2022Quote: ... and recombinant insulin human (≥ 98%) were all purchased from Sigma-Aldrich. Water (LC-MS grade) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, cat ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were coated with either recombinant GFP protein (Millipore) at 1 μg/mL to measure GFP-specific responses ...
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bioRxiv - Plant Biology 2021Quote: ... which yields recombinant proteins fused to hexahistidine tail (Novagen). The correct cloning was confirmed by sequencing.
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bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Biochemistry 2022Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG Fc specific antibody (Sigma, USA) was added at the dilution of 1:2,000 and incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... with goat anti-human Fc-specific IgG (Sigma-Aldrich – Saint Louis, MO) immobilized with a target capture level of ~70 RU ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). To enrich for MSCs ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). After blocking ...
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bioRxiv - Immunology 2022Quote: ... blocking of Fc receptors with human Ig (Sigma-Aldrich, St. Louis, MO); surface staining with mouse anti-human CD3-APC-H7 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). For analysis of neutrophil activation ...
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween-20 and 5% non-fat milk for 1 hour at room temperature and probed for 18 hours at 4 °C with antibodies specific for Flag tag (M2, SIGMA), Myc tag (9E10 ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...
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bioRxiv - Physiology 2020Quote: ... then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma Aldrich) at ZT6 (time “0 minutes”).
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bioRxiv - Biochemistry 2021Quote: Human recombinant SRPK2 (0.4 ug/reaction; EMD Millipore 14-666; Glu46-end) and SRSF2 (0.8 ug/reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 400ng/mL of human recombinant leptin (Sigma #L4146). In leptin neutralization studies ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Immunology 2020Quote: ... serum-free CHO Ex-Cell (Sigma # 14361C) media supplemented with 8 mM L-glutamine ...
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bioRxiv - Evolutionary Biology 2022Quote: Chinese Hamster Ovary cells (CHO-K1; Sigma) were cultured and transfected in vented T-25 flasks at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2022Quote: ... Chinese Hamster Ovary cells (CHO-K1, Sigma) stably expressing either pcDNA3.1(+ ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... The recombinant protein was produced in Rosetta (DE3) cells (Novagen) and purified by affinity chromatography using a GSTrapFF column ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were created using the pET expression system (Novagen). The codon-optimized typical faeG gene of the original K88 (K88ac variant ...