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Citations for Millipore Sigma :
1901 - 1950 of 4463 citations for Recombinant Human IL12B Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Neuroscience 2019Quote: Mouse cortical neurons expressing extracellular HA-tagged Nrxn1α for 4 hr were treated with 0.75 μg/mL brefeldin A (Sigma-Aldrich, Cat #B5936) added to the coverslips in a 12-well plate containing conditioned neuronal culture medium ...
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bioRxiv - Microbiology 2019Quote: ... coli cells with a YFP-tagged genomic copy using a previously developed strain (Taniguchi et al., 2011) and grown under chloramphenicol selection (Sigma Aldrich, USA).
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Immunoprecipitation of FLAG-tagged host factors in clarified supernatants with anti-FLAG agarose beads (EZview™ Red Anti-FLAG M2 Affinity Gel, Sigma-Aldrich) was performed at 4°C for 45 minutes ...
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bioRxiv - Plant Biology 2024Quote: AM 810–813 plasmids (Table S2) encoding active and proteolytically dead (PD) GST-tagged AtATG4A and AtATG4B were used to transform Rosetta (DE3) cells (Novagen, 70954-3). Expression was induced overnight at 16°C using 0.25 mM IPTG (Hellobio ...
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bioRxiv - Immunology 2021Quote: ... and 10% (v/v) fetal calve serum (FCS)) with 1.6 mg collagenase (Sigma-Aldrich, St. Louis, MO, USA) and processed twice on a GentleMacs using the lung program (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with pyruvate and glutamine (Difco) and 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma-Aldrich) (D10) ...
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bioRxiv - Cell Biology 2022Quote: ... was maintained with Dulbecco’s minimal essential medium (DMEM) (Nacalai tesque) containing 10% fatal calf serum (FCS; Sigma-Aldrich). To differentiate the cells into myotubes ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Eurobio, Les Ulis, France) and 20 µM beta-mercaptoethanol (SIGMA, Saint Quentin Fallavier, France)30 ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the ACE2-Fc expression vector was transfected into Expi293T cells according to the manufacturer’s instructions (Sigma-Aldrich; L3287). Five days after transfection the supernatant was collected by first spinning cultures at 300 g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord specimens were then placed in glass tubes filled with Fluorinert (FC-770, Milipore Sigma, Darmstadt, Germany) for imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Neuroscience 2020Quote: ... Medium supplemented with the exosome-depleted FCS and antibiotics was subsequently filtered through a 0.22 and a 0.1 µM filter-sterilization device (Millipore). For EV isolation ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor tissue was dissected into fragments of 1-2mm3 and frozen in 90% fetal calf serum (FCS, Sigma) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Medium supplemented with EV-depleted FCS and antibiotics was subsequently filtered using 0.22 and a 0.1 µM filter-sterilization devices (Millipore). For EV isolation ...
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bioRxiv - Immunology 2019Quote: ... Monocytes were cultured in RPMI-1640 medium supplemented with 10% FCS and 1% sodium pyruvate (all from Sigma) in ultra-low attachment 6-well plates ...
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bioRxiv - Immunology 2019Quote: ... cells were counted at d0 and 0.4x106 cells/ml were seeded in differentiation medium (RPMI supplemented with 2.5 % FCS, 0.5% dimethylformamide (DMF, D4551, Sigma), L-glutamine and antibiotics) ...
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bioRxiv - Molecular Biology 2021Quote: Wildtype and ΔMETTL3 mES cells were maintained in DMEM supplemented with 15% FCS (ES-009-B, Millipore Sigma), 1X non-essential amino acids (11-140-050 ...
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bioRxiv - Genetics 2021Quote: ... They were transferred to digestion medium (HBSS with Ca2+ Mg2+, FCS 2%, DNase I 0.5mg/mL (Sigma-Aldrich) and collagenase IV 0.5mg/mL (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... The EV-containing eluates were pooled and supplemented with 10% heat-inactivated fetal calf serum (FCS; Sigma-Aldrich), or 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and resuspended in PBS containing 0.5 % FCS and subjected to FACS analysis (Guava EasyCyte, Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
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bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted single cells were collected in ADF plus 10% FCS and 9 µM Rock inhibitor (Y-27632, Sigma) to prevent anoikis ...
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bioRxiv - Genetics 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Capricorn, FBS-12A) and 1% penicillin/streptomycin (P/S, Sigma, P0781). The cultured fibroblasts were incubated at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... Serum-cultured cells were grown on 0.1% gelatin-coated plates in Serum ES media (GMEM, 10% FCS (Sigma), 2mM Glutamax ...
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bioRxiv - Genomics 2024Quote: ... 20% FCS (Autogen Bioclear, Calne, UK) and 1,000 U/ml of leukemia inhibitory factor (ESGRO-LIF, Chemicon/Millipore). For the Ring1B conditional deletion ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg/ml prolactin (mouse recombinant for qPCR, western blot, immunohistochemistry and contraction control; Sigma or Peprotech) or sheep pituitary prolactin for time-lapse and confocal imaging ...
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bioRxiv - Immunology 2019Quote: ... A subset of mice was treated with IP with recombinant DNase I (20 mg/kg, Sigma-Aldrich) 2 hours after LPS injection24 ...
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bioRxiv - Microbiology 2021Quote: ... enzymatic activity of recombinant CynD was measured at pH 8.0 using the Ammonia Assay Kit (Sigma-Aldrich). A concentration of 500 nM of CynD was used in all reactions with the following cyanide concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Digestion of extended E34TSP was done according to the recombinant Enterokinase user protocol TB150 Rev.C 0107(Novagen). The extent of proteolytic activity on our TSP was calibrated via SDS PAGE analysis.