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Citations for Millipore Sigma :
1901 - 1950 of 3280 citations for Recombinant Human BMP4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: HDX of transferrin was performed by diluting solid apo-human transferrin (Sigma-Aldrich to a concentration of 10 μM in equilibration buffer (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 μg/mL stock of human epidermal growth factor (hEGF; Sigma: E9644) was prepared in 0.22 μm filter-sterilized 10 mM acetic acid (Chem Lab ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: We prepared samples of four different iron compounds: transferrin (holo-transferrin human, Sigma), apo-transferrin (apo-transferrin human ...
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bioRxiv - Cell Biology 2023Quote: ... 5UI of hCG—human Chorionic Gonadotropin (CG10, Sigma-Aldrich, St. Louis, MI, USA) were applied to mice at 1pm on the third day of the protocol (46th hour after using PGMS) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human vinculin (mouse monoclonal, VIN-1, Sigma Aldrich, V9131; WB 1:1000), anti-human Hic-5 (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... These evaluations were performed by the commercial Human ELISA kits (Sigma-Aldrich, USA) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 ng of human urokinase-type PLG activator (uPA, Sigma-Aldrich) was added per well ...
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bioRxiv - Cell Biology 2023Quote: ReNcell VM immortalized human neural progenitor cells (hNPC) were purchased from EMD Millipore, initially derived from the ventral mesencephalon region of human fetal brains ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 50ng of esiRNA Human KCNH2 (Cat. EHU080951, Milipore Sigma) or siRNA Universal Negative Control per 5.0 x 104 hiPSC-CMs using Mission® siRNA Transfection Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with 270ng of esiRNA Human GJA1 (Cat. EHU105621, Millipore Sigma) or siRNA Universal Negative Control (Cat ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Pathology 2023Quote: ... the anti-human CD31 primary antibody (clone JC70, Cell Marque, Millipore Sigma, USA), and the detection with impact DAB (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... and 1% (v/v) human serum from platelet poor plasma (P2918; Sigma-Aldrich) and seeded into a T75 cell culture flask that was previously coated with 0.1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... 2 μm latex green beads were incubated with human IgM (I-8260, Sigma), then in rabbit anti-human IgM (Sigma 270A ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.15×106 of murine stromal cells expressing human delta like ligand 1 (Sigma) were combined with 7.5×103 of sorted and enriched KO-/- ...
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bioRxiv - Biochemistry 2023Quote: ... Zymosan A was opsonized by incubation with human serum (Sigma-Aldrich, no. H4522) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... human iPSCs were cultured on gelatin-coated dishes in differentiation medium (GMEM (Sigma) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... MDM were cultured in RPMI 1640 medium supplemented with 10% human serum (Sigma). VSVDG pseudotyped HIVD1 GFP virus was produced by transfecting of 293T with GFPDencoding genome CSGW ...
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bioRxiv - Pathology 2023Quote: Human hepatoma cell lines were grown in Minimum Essential Medium (MEM, Sigma Aldrich) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... The shRNAs targeting human MARCHF8 were ordered from Sigma-Aldrich (St. Louis, MO). The sgRNAs targeting Marchf8 were designed using the web-based software ChopChop (chopchop.cbu.uib.no ...
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bioRxiv - Cell Biology 2023Quote: ... glass coverslips were coated with 10 µg/mL human plasma fibronectin (FC010, Millipore) overnight at 37◦C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected with 270ng of esiRNA Human KCNJ2 (Cat. EHU019661, Millipore Sigma) or non-targeting control (NTC ...
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bioRxiv - Microbiology 2024Quote: ... A final dilution of ∼105 CFU was mixed with human serum (Sigma-Aldrich, H6914 from male AB clotted whole blood ...
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bioRxiv - Immunology 2024Quote: ... and 10% human serum (heat-inactivated, sterile-filtered, male AB plasma; Sigma-Aldrich). Adaptive NK cell-positive donors were identified by anal-ysis of NKG2C (FAB138P or FAB138A antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... and human dermal fibroblasts were treated with 20 μM CCCP (Sigma-Aldrich, C2759), 5 μM valinomycin (Cayman Chemicals ...
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bioRxiv - Microbiology 2024Quote: ... which was the concentration used for the human IgG negative control (Sigma, I2511) in subsequent experiments ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Cell Biology 2024Quote: ... Dendritic cells were either cultured with 20 μg/ml human insulin (Sigma-Aldrich) to obtain immature DCs (iDCs ...
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bioRxiv - Molecular Biology 2024Quote: All human cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2024Quote: ... and a mouse monoclonal anti-human fibrin and fibrinogen (F9902, Sigma-Aldrich, USA) were incubated inside the flow chambers overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with rabbit anti-human IgM and Hoechst 33258 dye (Sigma) to visualize nuclei ...
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bioRxiv - Biochemistry 2024Quote: ... human Trx1 was concentrated to 2.5 mM using the Stirred Ultrafiltration Cell (Millipore) and buffered in PBS using PD-10 columns (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... primary human PBMCs were isolated by density gradient sedimentation in Histopaque® (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...