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Citations for Millipore Sigma :
1901 - 1950 of 2420 citations for RGS4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... the membranes were incubated with IRDye secondary antibody in Intercept® (PBS) Blocking Buffer with addition of 0.02% SDS and 0.2% Tween® 20 (Sigma, P1379) for an hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated again in blocking solution with primary antibody against choline acetyltransferase (ChAT; Millipore, AB144P, goat anti-ChAT, 1:1000) for 5 nights at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody reaction was carried out for 10 days at 4°C in blocking solution (1:1000 mouse monoclonal Tyrosine Hydroxylase antibody; T2928, Sigma-Aldrich). Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution and added to samples for 1 hour at room temperature (RT): ACTN1 (Sigma, A7811), TAGLN (Abcam ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Physiology 2019Quote: ... Sections were incubated in blocking solution (1%BSA: Sigma Aldrich, 10% normal horse serum: ThermoFisher Scientific, UK 0.3M glycine: Sigma Aldrich, Dorset UK ...
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bioRxiv - Cell Biology 2019Quote: ... Following incubation with the appropriate fluorophore-labeled secondary antibodies in blocking solution for 40 minutes in the dark and counter-staining with DAPI (Cat.# D9542-5MG, Sigma-Aldrich) with intervening washes with 0.2% Tween in PBS (PBST) ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were washed in PBS and subsequently blocked overnight at 4°C in blocking buffer (PBS containing 1% Triton X-100 (Sigma-Aldrich) and 5% goat serum (Gibco)) ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were rinsed 6 times in 1x PBS before being incubated with fluorescence-labeled secondary antibody (diluted in blocking buffer at 1:500 dilution ratio with 1/10000 Hoechst 33258, Sigma #94403) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with the primary antibodies diluted in a 1:1 solution of blocking buffer to PBS with 0.2% Tween (PBST) (Sigma-Aldrich, P7949) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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Epicardial slices: a 3D organotypic model for the study of epicardial activation and differentiationbioRxiv - Bioengineering 2020Quote: ... followed by permeabilization for nuclear antigens (0.1% Triton X-100 in PBS for 30 min) and blocking for 1 hour at room temperature with 20% Goat serum (Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... in phosphate buffered saline (PBS) at room temperature for 10 mins and treated with blocking solution (1.5% bovine serum albumin (Sigma-Aldrich, #A3059) / 0.1% fish skin gelatin / 0.1% Triton X-100 in PBS/NH4Cl ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were first incubated for 1 h at room temperature in a blocking solution containing 4 % bovine serum albumin (Sigma-Aldrich) in PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were incubated overnight at 4 °C with the indicated primary antibody prepared in blocking/permeabilization solution (with 0.1% Triton X-100): rabbit polyclonal antibody against GLUT-1 (1:250; Millipore, 07-1401), rabbit monoclonal antibody against HK-1 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with blocking solution (1% bovine serum albumin and 0.2% Triton-X in 0.01 m PBS) for 1 hour at room temperature before primary antibodies were applied in blocking solution [mouse anti-parvalbumin antibody (Millipore, MAB1572) at 1:2000 overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then permeabilized in 1×PBS supplemented with 0.1% Triton X-100 for 10 min followed by a blocking step in 1×PBS supplemented with 5% BSA (Sigma A7906). Cells were incubated with primary and secondary antibodies and ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating tissue sections were washed three times for 10 minutes with PBS then incubated for 2 h in a PBS-blocking solution containing 0.5 % Saponin (Sigma Aldrich 84510) and 10 % pre-immune goat serum (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma, I4506). Free active groups were blocked overnight with 0.5M Tris (pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blocking was followed by an overnight incubation at 4°C with antibodies against α-smooth muscle actin (αSMA) (clone 1A4, Sigma), Bip (ab21685 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight in blocking solution containing one of the following antibodies: Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152), Anti-Iba-1 (1/1000 ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated ON at 4°C with the following primary antibodies diluted in blocking buffer: anti-FLAG (1:200, M2 clone, catalog no. F1804, Sigma-Aldrich); anti-FLAG (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1h with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then incubated in a blocking buffer (5% normal goat serum, Vector; 5% normal donkey serum, Wako; 3% BSA, Sigma-Aldrich; and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blots were blocked with LI-COR Blocking Buffer for one hour at room temperature and then incubated for two nights with primary antibody (GFAP GA5 Sigma 63893) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed tissues were rinsed three times with PBST before incubation in blocking buffer (PBS with 5% goat serum; Sigma Aldrich, #G9023) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Licor Blocking buffer (Odyssey, Lincoln, NE) for 1h at room temperature and primary antibody (Kal-spec, Millipore Sigma, Burlington ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating VTA brain sections were incubated 1h at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% Bovine Serum Albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH ...
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bioRxiv - Immunology 2021Quote: ... antibody diluted 1:1000 in blocking solution or rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody (Sigma-Aldrich Cat# A2054, RRID:AB_257967) were added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated in blocking buffer containing 2.5% bovine serum albumin and 0.1% Triton X-100 (Sigma Aldrich, St Louis, MO) for 30 minutes at room temperature then incubated in a primary antibody dilution in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with primary antibodies in blocking buffer for 1h at 21°C: 1:1000 α-tubulin (118M4779V; Sigma Aldrich), 1:250 CREST (15-234-0001 ...
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bioRxiv - Immunology 2022Quote: ... Fixed cells were washed twice with PBS and the remaining PFA was inactivated with blocking buffer (PBS-2% BSA (Sigma: #A9418), 1.5M Glycine (Sigma ...
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bioRxiv - Pathology 2020Quote: ... The blocking solution used for the anti-phospho-Smad2 and anti-phospho-Smad3 antibodies contained 5% bovine serum albumin (Sigma-Aldrich). The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2020Quote: ... after several washes in PBS explants were incubated at room temperature for 1 hour in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and 1:400 for TRITC-conjugated phalloidin (Sigma-Aldrich)) and then mounted using Fluoromount-G (Southern Biotech).
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Immunology 2020Quote: ... The IHC protocol mirrors the MIBI-TOF protocol with the addition of blocking endogenous peroxidase activity with 3% H2O2 (Sigma Aldrich) in ddH2O after epitope retrieval ...
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bioRxiv - Genomics 2019Quote: ... Cells were incubated with primary antibody diluted in the blocking buffer for 2 hours [alpha-tubulin mouse mAb (1:5000, Sigma T6199), Rad21 rabbit pAb (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
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bioRxiv - Cell Biology 2020Quote: ... the channel was washed three times with 10 μl nuclease-free water and the channel walls were passivated with 10 mg/ml Western Blocking Reagent (Sigma Aldrich) in PBS for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently incubated for 1 hr at room temperature in a blocking solution consisting of PBS with 5% Goat Serum (Sigma-Aldrich). Following blocking ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic protein G beads were washed with blocking solution (0.5% BSA in PBS) and then incubated with rabbit antiserum against acetylated histone H4 (Millipore 06-866) or normal rabbit anti-serum in blocking solution for several hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were blocked in blocking buffer (5% skim milk, 0.1% Tween in PBS) and incubated with β-Actin (#A5316, Sigma-Aldrich), MYC (#9402 ...