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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-His-specific monoclonal antibodies were from EMD Millipore (prod # 70796). Anti-T7 tag-specific monoclonal antibodies were from Novagen.
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bioRxiv - Immunology 2024Quote: ... HIS-Select® nickel affinity gel (P6611, Sigma-Aldrich, Darmstadt, Germany) was applied according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... His-mNeonG-RanGTP was purified using Rosetta(DE3)pLysS cells (Novagen) grown with 50 mg/mL kanamycin and 25 mg/mL chloramphenicol ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... extracts were redissolved in 20 µl of n-hexane containing 1 µg/ml n-nonane (Sigma-Aldrich) as an internal standard and vortexed at maximum speed for 10 seconds ...
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bioRxiv - Biophysics 2022Quote: ... encoding CPB was cloned as N-terminal His6 or N-terminal GST fusion into pET19-b (Novagen) using NcoI and BamHI sites ...
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bioRxiv - Cell Biology 2020Quote: ... macropinocytosis inhibitor 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) from Sigma-Aldrich (A3085, used at 25 µM). Other ...
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bioRxiv - Neuroscience 2020Quote: ... Trim28-fl(+/− and +/+) animals (Ctl n=5, KO n=5) were put in RIPA buffer (Sigma-Aldrich) containing Protease inhibitor cocktail (PIC ...
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bioRxiv - Cell Biology 2021Quote: ... The dye solution is prepared dissolving the dye powder in anhydrous N,N-dimethylformamide (DMF, Sigma-Aldrich). The dye to protein molar ratio was adjusted between 2 - 5 in order to provide a DOL around 1 ...
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bioRxiv - Microbiology 2020Quote: ... gondii were mutagenized with ENU (N-Ethyl-N-nitrosourea, N3385-1G, Sigma Aldrich; 100-200 μg/ml) in DMEM without FBS for 2 hours at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerization of 1 M monomer was performed with 4 mol% N,N’-methylene-bis-acrylamide (Sigma-Aldrich) as a crosslinker with 0.1% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... was used at 100 µM, amiloride (inhibit micropinocytosis, 5-(N-Ethyl-N-isopropyl, catalog number A3085, Sigma) at 50 µM and zoniporide hydrochloride hydrate (inhibit micropinocytosis ...
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bioRxiv - Microbiology 2020Quote: ... Evans blue dye was extracted from the organs by the addition of N,N-dimethylformamide (Sigma-Aldrich) (adjusted to 4 mL/g wet tissue ...
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bioRxiv - Biophysics 2020Quote: Unsized unilamellar DOPG liposomes containing Laurdan (6-Dodecanoyl-N, N-dimethyl-2-naphthylamine, from Sigma, Taufkirchen, Germany) (molar ratio lipid:Laurdan=1:500 ...
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bioRxiv - Microbiology 2020Quote: ... We then prepared a solution of 457 mM N,N-Bis(carboxymethyl)-L-Lysine-hydrate (Sigma-Aldrich) in 1 M NaHCO3 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by addition of 100 µl N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide + 1% tert-Butyldimethylchlorosilane (Sigma Aldrich, 00942) for 1 hour at 60°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT D5942, 1μM, Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... with the addition of N-methyl-N-t-butyldimethylsilyltri-fluoroacetamide (MTBSTFA) (Sigma-Aldrich, St. Louis, MO, USA) (Richardson ...
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bioRxiv - Biochemistry 2022Quote: ... Methoxaymine hydrochloride (#226904, MOX) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (#694709, MSTFA) were purchased from Sigma Aldrich. Protease inhibitor cocktail (#786-437 ...
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bioRxiv - Neuroscience 2022Quote: ... The interior walls of the capillaries were silanized with silan vapors (N,N-Dimethyltrimethylsilylamine, Sigma Aldrich, France) for 15 min and dried at 200°C for 100 min ...
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bioRxiv - Microbiology 2022Quote: ... the dried aliquot of eluate 1 was dissolved in 100 µl N-methyl-N-(trimethylsilyl)-trifluoroacetamide (Sigma) and incubated in a shaking incubator at 110 rpm (30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Evans Blue was extracted from tissue pieces for 48 hours in N,N-Dimethylformamide (Sigma, cat. #D4551) at 55°C ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were mounted in glycerol / n-propyl gallate medium (4% n-propyl gallate (02370, Sigma-Aldrich), 8 ml 100% glycerol ...
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bioRxiv - Bioengineering 2023Quote: ... fully dissolved in a solvent system of 60/40 volume ratio of N,N-dimethylformamide (DMF) (Sigma) to acetone (Fisher) ...
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bioRxiv - Microbiology 2024Quote: Chemical labeling with pseudouridine specific CMCT (N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate (Sigma-Aldrich) reagent was pursued to identify and quantify pseudouridine modifications in B ...
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bioRxiv - Biochemistry 2020Quote: ... the phosphorylated self-complementary oligonucleotides containing sequences of MccA1Hmi and MccA2Hmi ORFs were inserted into pRSFDuet-1 vector (Novagen-Millipore, USA), digested with NcoI and HindIII ...
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bioRxiv - Biochemistry 2020Quote: ... the phosphorylated self-complementary oligonucleotides containing sequences of MccA1Hmi and MccA2Hmi ORFs were inserted into pRSFDuet-1 vector (Novagen-Millipore, USA), digested with NcoI and HindIII ...
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bioRxiv - Cell Biology 2021Quote: ... Human full length SPAG5 ORF (ATG1-SPAG5) or excluding the first 453 nucleotides (Δ151-SPAG5) were cloned into p3xFLAG-CMV-14 (Sigma-Aldrich) using NotI or NotI/ClaI restriction sites ...
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bioRxiv - Neuroscience 2024Quote: The tcima ORF was cloned into the BamHI/EcoRI sites in the pGEX-4T-1 backbone (Sigma cat# 28-9546-49). The zebrafish tcima ORF was PCR amplified from cDNA generated from 5 dpi wildtype SC using the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tag was detected with peroxide–anti-peroxide complexes (P1291; Sigma). Detection was performed using the enhanced chemiluminescence procedure (ECL kit).
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bioRxiv - Cell Biology 2021Quote: ... anti-MYC (for MYC tag) (Sigma, St. Louis, MO, cat # C3956).
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bioRxiv - Plant Biology 2021Quote: ... and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... The GST tag was cleaved with thrombin (commercial thrombin, Sigma Aldrich), at 200 units per mL for 2h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Molecular Biology 2024Quote: ... and validated by Western Blotting for 3xFLAG tag (Antibody M2, Sigma).
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt, Germany /Sigma Aldrich, St. Louis, MO, USA), Spot tag (ChromoTek ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Neuroscience 2023Quote: The StrepII–GFP tag was cleaved by HRV 3C protease (Novagen) added at 10 U/mg of BmGr9 overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Flag tag (F3165) and C14ORF43 (HPA003111 for WB) from Sigma-Aldrich. Monoclonal antibodies against TFII-D subunits were generous gifts from Dr ...