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Citations for Millipore Sigma :
1901 - 1950 of 2557 citations for PSMD6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... for 15 min and then cell blocking solution (5% w/v BSA, purchased from Millipore Sigma, dissolved in 1x DPBS) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were then washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% BSA (Sigma), 1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h in a 10% fetal bovine serum (FBS)/PBS blocking buffer containing 0.5% Triton X-100 (cat# X100, Sigma-Aldrich), and incubated overnight at 4°C with the following primary antibodies diluted in the blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue sections were stained with primary antibodies in blocking buffer (PBS + 0.3% Triton-X100 + 1% BSA (cat. # B2518, Sigma-Aldrich)) for 48 hours on a rocker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then rinsed with rinsed with 0.1% Triton X-100 in PBS (0.1%PBST) and then blocked with blocking buffer consisting of 5% normal goat serum (G9023, Sigma-Aldrich) in 0.1% PBST for 1hr at RT ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: Mounted sections were incubated for 1 hour at room temperature with and blocked using blocking buffer containing (Sigma-Aldrich, #G9023) and 0.1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1h at RT in blocking buffer (BB: 10% Bovine Serum Albumin 0.3% Triton™X-100 (Sigma-Aldrich) in 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibody (Rabbit anti-GFP 1:1000 in blocking buffer, Cat# AB3080, Millipore Sigma, St. Louis, MO) overnight at room temperature on an orbital shaker ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X-100 (Sigma) for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were immersed in blocking solution (5% (vol/vol) horse serum in phosphate buffer with 0.1% (vol/vol) Triton X-100 (Sigma; X100)) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent a 2h incubation at room temperature (RT) in a blocking solution consisting of 0.1M PBS supplemented with 5% (v/v) goat serum (Sigma; G9023) and 0.3% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Neuroscience 2024Quote: ... Sections then were incubated in blocking buffer (10% normal donkey serum in 0.5% PBST, Sigma-Aldrich, catalog no: D9663-10ML) for 1 hour at RT before incubating in primary antibody solution (2% normal donkey serum in 0.4% PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were permeabilized with 0.5% Triton-X100 in PBS for 5 min at RT and a blocking solution (PBS supplemented with 5% BSA (Sigma) and 0.1% Triton-X100 ...
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bioRxiv - Microbiology 2024Quote: ... was separated from the insert and blocked and permeabilized with a blocking buffer containing 2% Bovine Serum Albumin (BSA) (Serva) and 0.1% saponin (Sigma Aldrich) before incubating with the primary and secondary antibodies at 4°C in dark ...
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bioRxiv - Molecular Biology 2024Quote: ... Permeabilization was then performed by addition of a solution of 0.5% Triton X-100 (AppliChem, A1388,0500) for 30 min before addition of blocking solution (BSA 1% (Sigma-Aldrich, A7906) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were blocked and permeabilized in blocking buffer (10% donkey serum [Jackson ImmunoResearch 017-000-121] in PBS with 0.3% Triton [Sigma T9284] and 100mM L-lysine [Sigma L5501] ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...
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bioRxiv - Immunology 2024Quote: ... Cell permeabilization was performed for 10 min with 0.25% Triton X-100 in PBS followed by blocking with 5% normal goat serum (Sigma- Aldrich) for 2 h at room temperature and overnight incubation with anti-CD3 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 1 h incubation with secondary antibodies also diluted in blocking buffer8 Immunostained tissue sections were counterstained with DAPI (1:1000 in PBS) and were mounted in Mowiol (Millipore). Other immunohistochemical stainings were performed as previously described52 using antigen retrieval in 0.01 M sodium citrate buffer (pH 6.0 ...
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bioRxiv - Biophysics 2020Quote: The lyophilized linear peptide (4.1 mg, 1 μmol) was dissolved in 100 ml refolding buffer containing 100 μmol reduced glutathione (GSH, Sigma), 10 μmol oxidized glutathione (GSSG ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... beads were washed 3X with 0.1% Tween20/TBS and 3X with TBS before elution with 3XFLAG peptide (Sigma F4799). Gel loading buffer was added to the eluates for SDS-PAGE electrophoresis and Western blotting analysis.
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bioRxiv - Cell Biology 2020Quote: ... digested sample was dried in a vacuum concentrator and reconstituted in 0.1% TFA + 1 picomole/uL Angiotensin-II peptide (Sigma). 1% of the sample was subjected to LC-MS/MS/MS analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Biochemistry 2019Quote: ... The peptide digest was dissolved in 21 μL of LC-MS grade water containing 0.1% formic acid (F0507-100ML, Sigma). The digest was filtered with a 0.65 µm pore-size micro-centrifuge filter before it was loaded to an HPLC auto-injection sample.
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... Murine BMDCs at day 8 of cultivation were incubated with 3-fold higher numbers of CD4+ CD25− T-cells (OT-II or OT-II.RAG-1-/-) and 200 ng/ml OVA peptide (323-339, grade V, Sigma) supplemented or not with our different stimuli ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Bioengineering 2019Quote: ... The gelatin and peptide solutions were prepared by dissolving in deionized water (supplied by a Millipore Milli-Q system) at 10 wt % by heating at 50°C for 30 min ...
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bioRxiv - Biophysics 2019Quote: ... The BBSome:ARL6 complex was eluted from the column with a total of 10 ml of 0.1 mg/ml Flag peptide (Sigma). Elution was performed in 5 steps ...
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bioRxiv - Cell Biology 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich, F4799)) was added and incubated with beads overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.4, 200 mM NaCl, 2 mM MgAc2, 0.25 mg/mL 3X FLAG peptide -Sigma F4799) with gentle end-over-end mixing ...
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bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by a peptide clean-up as per the zip-tip protocol (Millipore Sigma; Cat# C18 ZTC18S008). 20ul of 0.1% formic acid (in acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared in 1:1 mixture of H2O-ACN with 0.1% TFA by mixing an equal amount of peptide fractions (0.5 μl) with α-cyano-4-hydroxycinnamic acid (CCA)/2,5-dihydroxy benzoic acid (DHB) matrix (Sigma) and spotted on a stainless-steel plate and air-dried ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
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bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
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bioRxiv - Cell Biology 2021Quote: ... and kinetochores particles were eluted by gentle agitation of beads in elution buffer (Buffer H + 0.5mg/mL 3FLAG Peptide (Sigma)) for 30 min at room temperature.
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were acidified with 1% trifluoroacetic acid (TFA) and then desalted using C18 ZipTip® (Millipore cat # ZTC18 5096). Dried peptides were resuspended in 10 µL of 0.1% TFA in water ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were eluted by incubating the beads with 35 μl 100 µg/ml 3xFLAG-Peptide (F4799, Sigma Aldrich) at room temperature for 20 min or by adding Laemmli Sample Buffer (1610747 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding the selected peptides were isolated in 96-well format using the Montage Plasmid MiniprepHTS Kit (MilliPore, LSKP09604) on a MultiscreenHTS Vacuum Manifold (MilliPore ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 100 μL of buffer (50 mM Tris, 150 mM NaCl, 1mM EDTA, pH 7.4) with Flag peptide (Sigma) and rocked overnight at 4°C overnight ...