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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Biotin Z Antibody Internalization Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-αSMA (Cat: A5228) and mouse monoclonal anti-β-actin (AC-74) (A2228) antibodies were from Sigma (St Louis, MO). Anti-TGFβR1 (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl of washing buffer supplemented with 6 mM free biotin (Sigma) was added to the beads ...
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bioRxiv - Neuroscience 2020Quote: ... cell culture media was supplemented with 50 μM biotin (B4639, Sigma-Aldrich). The next day ...
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bioRxiv - Biophysics 2021Quote: ... For liposome experiments channels were coated with BSA-biotin (Sigma Aldrich #A8549). For direct surface attachment microscope slides and coverslips were coated with a 100:1 PEG/PEG-biotin mixture (Laysan Bio #BIO-PEG-SVA-5K & MPEG-SVA-5K ...
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bioRxiv - Neuroscience 2022Quote: ... Biotin 3-sulfo-N-hydroxysuccinimide ester (1 mg/mL; Sigma, Cat# B5161) was added for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... followed by incubating with WB1 supplemented with 5 mM biotin (Sigma-Aldrich) at RT for 15 min to block free biotin-binding sites on the beads and then washing with 1 ml of WB1 for 3 times ...
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bioRxiv - Immunology 2022Quote: ... excess biotin was removed using Amicon Ultra 0.5L columns (3K, Millipore Sigma). The biotinylated whole-cell lysate was then added to FITC neutravidin beads (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... the transiently transfected HEK293T cells were treated with 50uM Biotin (Sigma-Aldrich) for 12 hours ...
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bioRxiv - Neuroscience 2019Quote: ... 5μM forskolin and 1ng/ml biotin (all Sigma-Aldrich unless otherwise indicated). Cultures received growth factors for the first 4 days (10ng/ml/day of platelet-derived growth factor α (PDGFα ...
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bioRxiv - Plant Biology 2019Quote: ... For biotin depletion with an Amicon Ultra-4 Centrifugal filter (Millipore Sigma), 625 μl protein extract were concentrated three times to 10 – 20% of the starting volume by centrifuging in a swinging bucket rotor at 4,000g and 4°C and diluted with extraction buffer to the initial volume ...
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bioRxiv - Immunology 2021Quote: ... and 10uL of 1M NaOAc and 22uL of 50mM hydrazide biotin (Sigma) were added ...
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bioRxiv - Genomics 2022Quote: ... Biotin-labelled HSAT1 probe was detected with Streptavidin Cy3 conjugated (Sigma-Aldrich). Preparations were mounted using Vectashield containing 4’-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2022Quote: ... Expamer stimulus was disrupted by incubation with 1 mM D-biotin (Sigma). 1×106 cells were electroporated (pulse code EH100 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with biotin-conjugated WFA lectin (1:500; Sigma-Aldrich), MECP2 primary antibody (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... After dialyzing 3 times in PBS DEL-biotin incubated with streptavidin (Sigma) at a 10:1 molar ratio for 30 minutes on ice ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated in the presence of 100 µM biotin (Sigma-Aldrich) to release the cargo proteins ...
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bioRxiv - Genomics 2023Quote: ... 2.5 μL 20 mM DBCO-PEG4-biotin (Sigma, Cat # 760749; DMSO solution), and 10 μL 10 × PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The Azide-647 was replaced with Azide-PEG3-Biotin (Sigma, 10 mM) and supplements with 6 mM THTPA (Jena Bioscience).
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bioRxiv - Cell Biology 2023Quote: ... resuspended in biotin binding buffer (5 mM TRIS-HCl pH 7.5 (Sigma), 0.5 mM EDTA (AppliChem ...
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bioRxiv - Genomics 2023Quote: ... 2.5 µL 20 mM DBCO-PEG4-biotin (Sigma, Cat # 760749; DMSO solution), 10 µL 10 × PBS ...
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bioRxiv - Immunology 2023Quote: ... Anti-β-actin-peroxidase (A3854) and biotin (B4639) were from Sigma-Aldrich. Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... OD600 = 0.5 the cells were treated with 50μM biotin (Sigma-Aldrich, B4501) and harvested when the cultures reached OD600 =1 ...
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bioRxiv - Cell Biology 2024Quote: ... PBST) and then incubated with 20µl biotin-agarose beads (Sigma, MO, USA).
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bioRxiv - Neuroscience 2024Quote: ... and Wisteria floribunda agglutinin biotin-conjugated lectin (WFA, Sigma, L1516, RRID: AB_2620171).
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with media containing 50 µM biotin (Sigma Aldrich, #B4639) for 10 min at 37°C and 5% CO2 for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Z-VAD(OH)-FMK (caspase inhibitor VI; SelleckChem S8102; 453.5 MW) and Staurosporine (STS; Sigma S5921; 466.5 MW).
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bioRxiv - Biochemistry 2022Quote: ... eluted C3-SC1TECs were pre-warmed at 37 °C for 2 minutes before 19.5 μl of C3-SC1TECs were mixed with 5.5 μl of pre-warmed Chase Mix Z (2.5 μl 5 mM NTPs, 2.5 μl 100 μg/ml rifampicin, 0.5 μl of ZMP (Sigma Aldrich) in DMSO at 50X final ZMP concentration) ...
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bioRxiv - Immunology 2022Quote: ... endpoints A and Z) added with 498 µl of RPMI 1640 culture medium (Sigma-Aldrich, Saint Louis, Missouri) and 2 µl of pH 5 citrate-phosphate buffer excipient (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... cell cultures were treated with a 100 mM stock of MG132 (Z-Leu-Leu-Leu-al, C2211, Sigma) in DMSO to achieve a final concentration of 100 µM ...
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bioRxiv - Cancer Biology 2022Quote: ... Setd2flox/flox MEFs expressing ER-Cre were treated with 3μM (Z)-4-hydroxytamoxifen (4-OHT, Millipore Sigma, H7904) or vehicle (Ethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... For fibronectin deletion (FN-/-) one metanephric kidney was treated with (Z)-4-hydroxytamoxifen (HT; 500 nM, Sigma-Aldrich) whereas the contralateral kidney was treated with control medium only and served as control (FN+/+) ...
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bioRxiv - Immunology 2024Quote: ... cells were pre-treated for 30min with 100µg/mL Z-VAD-FMK (Sigma-Aldrich, St. Louis, MO, USA), 1µg/mL cytochalasin D (Santa Cruz Biotechnology Inc ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Genetics 2019Quote: ... paraffin-embedded and stained using anti-RAS (G12S) mouse monoclonal antibody (NewEast, 26186) followed by incubation with HRP-conjugated corresponding secondary antibody (Sigma-Aldrich). The expression levels were evaluated by H-score method ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with primary antibodies: mouse monoclonal antibody against α-actinin (1:1,000; Millipore, MAB1682), mouse monoclonal antibody against G6PD (1:250 ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with blocking solution (1% bovine serum albumin and 0.2% Triton-X in 0.01 m PBS) for 1 hour at room temperature before primary antibodies were applied in blocking solution [mouse anti-parvalbumin antibody (Millipore, MAB1572) at 1:2000 overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at the following dilutions: horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Sigma, 1:20,000), anti-mouse Oregon Green (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... Then cells were stained with anti-mouse IgG Taxes red conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACS Aria II (BD ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Microbiology 2019Quote: ... The TopA protein was subsequently detected using primary rabbit polyclonal anti-TopA antibodies and mouse secondary anti-rabbit IgG antibodies conjugated with alkaline phosphatase (Sigma Aldrich), according to the procedure described previously (Szafran et al. ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...