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Citations for Millipore Sigma :
1901 - 1950 of 8155 citations for BD 3 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... Slides were incubated for 3 minutes in 3% acetic acid and then for 20 minutes in Alcian blue solution (Sigma Aldrich) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... then quickly was cut to small pieces (1-3 mm) in petri dish with 3 -5 ml RMPI medium containing 0.05mg/ml TM Liberase (Sigma Aldrich, 5401119001) and 0.02 mg/ml DNAaseI (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... (1) A modification solution was made by dissolving 10 mM of 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, Sigma-Aldrich Inc.) and 75 mM of N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was placed in 99.8% ethanol and LR White resin (at a ratio of 3:1, 1:1, 1:3; Sigma Aldrich, USA), and next in 100% resin LR White ...
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bioRxiv - Plant Biology 2024Quote: ... where 10 seedlings each were incubated 2.5-3 h in the dark at RT with agitation in 3 ml of liquid medium (same as above) supplemented with either 3 µl of 1 mM Concanamycin A (Sigma, C9705) dissolved in DMSO or with 3 µl DMSO alone (control) ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Biophysics 2020Quote: ... cells were plated sparsely on glass coverslips coated with rat-tail collagen I (Cat # 3867, Sigma) at a coating density of 10 µg/cm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Male Wistar rats were intravenously (IV) injected with puromycin aminonucleoside (PAN) (Sigma-Aldrich, St. Louis, MO) and treatment groups were treated by oral gavage with Pioglitazone (Alfa Aesar ...
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bioRxiv - Immunology 2021Quote: ... Rat splenocytes (2 × 105) were plated in duplicate in 96-well PVDF plates (Millipore, Billerica, MA) and stimulated with 5 μg/ml of recombinant 2019-nCoV Spike protein (S1+S2 ECD ...
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bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were labelled with the appropriate set of antibodies: primary rat anti-HA (1:500, Sigma), mouse anti-Myc (1:1000 ...
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bioRxiv - Plant Biology 2019Quote: ... and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P, 1:10,000, Sigma). HRP activity was detected by the Supersignal Western Detection Reagents (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... rats were pretreated with atropine methylbromide (5 mg/kg, i.p.; Sigma-Aldrich, Inc.; St. Louis, MO) 20 min before seizure induction with pilocarpine hydrochloride (345 mg/kg ...
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bioRxiv - Synthetic Biology 2019Quote: ... Supernatants and lysates were separated by SDS-PAGE followed by immunoblotting with rat anti-HA (Sigma) antibody to evaluate the presence of recombinant CD47nb protein in culture fractions ...
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bioRxiv - Genetics 2021Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
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bioRxiv - Developmental Biology 2021Quote: ... the uterus was excised from the mated female rat and flushed with M2 medium (M7167, Sigma). The flushed blastocyst-cell stage embryos were selected under a dissecting microscope and rechecked by an inverted microscope (BM ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were against β-III tubulin (Tuj-1; mouse anti-rat, 1:300; Millipore), choline acetyltransferase (ChAT ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were used at the following concentrations: rat anti-HA 3F10 at 1:400 (Sigma-Aldrich), mouse anti-Myc 9B11 at 1:2000-1:3000 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then allowed to bind to affinity-purified rat anti-HA antibody (Sigma) attached to paramagnetic beads (Dynabeads protein G ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with primary antibody rat anti-somatostatin (1:250, Millipore cat. No. MAB354, RRID:AB_2255365) at RT for 24 hours and then 4°C for 42 hours ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253 ...
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bioRxiv - Bioengineering 2020Quote: ... P0-1 SD rat brains were removed and rinsed in phosphate buffer saline (PBS, Sigma-Aldrich). After careful removal of the meninges and brains cortices ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma) and fibronectin (5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived secondary antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963), the goat-derived secondary antibody (anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Collagen type I solution from rat tail (C3867-1VL) and collagenase (C9263) were purchased from Sigma–Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-rat PLA plus and minus probes were made using the PLA probemaker kit (Sigma Aldrich) with a Goat anti-rat IgG antibody (Jackson Immunoresearch ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Neuroscience 2021Quote: Mouse neuroblastoma x rat neuron hybrid ND7/23 cells were purchased from Sigma-Aldrich (ECACC 92090903). They were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... Rats were initially trained to lick for sodium saccharin solution (0.2% in distilled water; Sigma 47839) during 60 min sessions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Biochemistry 2020Quote: ... The shRNA lentiviral plasmids (pLKO.1-puro) for human/mouse/rat frataxin were purchased from Sigma. The RefSeq used was NM-008044 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A single dose (100µl in rats, 20µl in mice) of complete Freund’s adjuvant (CFA, SIGMA Aldrich) was injected subcutaneously into the plantar surface of one hind paw using a microliter syringe with a 27-gauge needle ...
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bioRxiv - Neuroscience 2021Quote: ... female rats were subcutaneously injected with 1 mg progesterone (5 mg/mL; Sigma, St. Louis, USA) in peanut oil (Apotekproduksjon ...
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bioRxiv - Cell Biology 2021Quote: ... substrates were coated with 10 μg/cm2 collagen type-I from rat tail (Sigma, Cat # C3867) overnight at 4°C.
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bioRxiv - Cell Biology 2020Quote: ... E18 rat striatal neurons were obtained from BraintBits LLC and cultured on poly-d-lysine (Sigma) coated coverslips for 1 week in Neurobasal media (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived second antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963) is available as a commercial product.
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bioRxiv - Immunology 2022Quote: ... 24 well tissue culture plates were pretreated with 0.2mg/ml of collagen from rat tail (Sigma; 1mg/ml stock diluted in 0.1M acetic acid ...
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bioRxiv - Neuroscience 2023Quote: ... rats were injected with 30 mg/kg fluorescein isothiocyantate (FITC)-dextran (500 kDa, Sigma catalog #FD500S) to label the cerebrovasculature ...
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bioRxiv - Neuroscience 2023Quote: ... Rats were pre-treated intraperitoneally (i.p.) with 20 mg/kg of desipramine hydrochloride (Sigma, Poole, UK) in order to preserve noradrenergic neurons ...
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bioRxiv - Plant Biology 2022Quote: ... Exposure to primary antibodies (rat - anti GFP 3H9-Proteintech or mouse – anti Biotin B7653-Sigma-Aldrich) was done for 2 hours at 25°C ...