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Citations for Millipore Sigma :
1901 - 1950 of 3014 citations for 6 Chlorohexanenitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... 6-Diazo-5-oxo-L-norleucine (DON) was used at final concentration of 2,5µM (Sigma Aldrich, ref. D2141). 2-Desoxy-D-glucose (2-DG ...
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bioRxiv - Cancer Biology 2024Quote: ... we used 2’,7’-Bis (2-carboxyethyl)-5 (6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) (Sigma-Aldrich, Darmstadt, Germany). Cells were starved overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and after cool down alkylated with 6 μL of freshly prepared 1 M iodoacetamide (IAA, Sigma, #I1149-5G). The alkylation was performed for 15 min at room temperature in darkness ...
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bioRxiv - Biophysics 2024Quote: ... 90,000 U2OS cells/well were seeded in 6-well with 8 ug/ml polybrene (Millipore #TR-1003-G) addition to the growth medium and infected with 4ul of lentiviral particle containing supernatant ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 sec) and then vapor silanized with Trichloro (1H,1H,2H,2H-perfluoro-octyl) silane (Si; Sigma, 448931). To make the 1st PDMS ...
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bioRxiv - Microbiology 2024Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Crosslinked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... Cross-linked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Biophysics 2021Quote: ... an enzyme mixture was made with 0.1 µM GOx-488 and 0.1 µM GOx-532 in a 50 mM MES (pH 6; Sigma-Aldrich) buffer solution ...
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bioRxiv - Biophysics 2021Quote: Cells were grown in 6-well plates in RPMI1640 or DMEM media supplemented with 10% (v/v) FBS (Sigma), 2 mM glutamine (Gibco) ...
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bioRxiv - Biophysics 2020Quote: Transfection of 1 × 105 293T cells per well of a 6-well plate was carried out using GeneJuice (Novagen) transfection reagent as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre-soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 cells were plated in 6-well poly (2-hydroxyethyl methacrylate) (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA)-coated plates for the indicated amount of time in the figure legends ...
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bioRxiv - Cell Biology 2020Quote: ... Three-month-old C57BL/6 male mice were injected with 10 µg BrdU (Sigma-Aldrich, St. Louis, MO; www.sigmaaldrich.com)/gram body weight intraperitoneally every 12 hours for 2 days before sacrifice ...
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bioRxiv - Cell Biology 2020Quote: ... and (1uM) Clemastine Fumarate D3:D20 (Cayman) for 6 weeks (M2; the optimal media) and (2.5ug/ml) Laminin (Sigma), and (1 ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Genetics 2021Quote: ... and embryos from natural spawning were injected with up to 600pg RNA in phenol red (P0290, Sigma, 1:6). Injected embryos were maintained at 28.5°C in egg water and collected at 26hr for histology ...
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bioRxiv - Neuroscience 2020Quote: ... Each IHC staining round was accompanied by nuclear cell counterstaining with 4’,6-diamidino-2-phenylindole (1μg/ml DAPI (Sigma) diluted 1:1000 in PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Microbiology 2022Quote: ... the larvae were kept in 6-well plates containing egg water (60 μg/mL sea salts (Sigma-Aldrich; S9883) in demi water ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was visualized by FluoProbes 547H (557/572nm) coupled Phalloïdin (Interchim) and nuclei with 0.2 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted in Mowiol (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated at a density of 6×104 cells / cm2 on glass coverslips coated with 0.2mg/ml Poly-L-lysine (#P2636-100MG Sigma) made in Borate buffer pH 8.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The neurons were quantified and centrifuged at 1,800 rpm for 6 min and then plated on the glass coverslips pre-coated with poly-L-lysine (Sigma). The culture medium was Neurobasal (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed in a pre-hybridization solution (6 x SSC, 1 x Denhardt’s, 100 μg/ml denatured Herring Sperm DNA (Sigma) and 0.5 % SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... Reconstituted complexes were purified over a Superose 6 increase 3.2/300 size exclusion column SEC in GF150 buffer and immediately crosslinked in 0.025% Glutaraldehyde (Sigma-Aldrich) for 30 minutes on ice followed by quenching with 1 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 37°C with daily medium change and split twice a week in a 1:6 ratio using 0.05% Trypsin-EDTA (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... the nucleus was stained by incubation with 4’,6-diamidino-2-phentylindole (DAPI, Sigma, 200 ng/ml in PBS) for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, REF-D9542, Sigma, USA) for 30 min at RT ...
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bioRxiv - Physiology 2019Quote: ... The study included five treatment groups (n≥6 per group) receiving either 0.24 g/kg bodyweight bovine fetuin-A (Sigma) or 0.10 g/kg bodyweight PPi (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and kinate receptors was achieved with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX; 50 µM; Sigma, Australia), N-methyl-D-aspartate (NMDA ...
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bioRxiv - Plant Biology 2019Quote: ... Fe(III)-cellulose (5 mM FeCl3 + 50 mM cellulose, pH 5.8, bought from Sigma, CAS number: 9004-34-6), Fe(III ...
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bioRxiv - Physiology 2019Quote: ... Eight-week-old male C57Bl/6 mice (Janvier Laboratories, France) were used for pharmacological experiments with spironolactone (Sigma-Aldrich), eplerenone (Inspra ...
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bioRxiv - Immunology 2019Quote: ... spleens from C57BL/6 mice were cut to smaller pieces and digested with Collagenase D (2 mg/ml, Sigma) For 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in complete Neurobasal media and plated at 0.5 × 106 cells/well on 6-well plates coated with 0.1 mg/ml poly-L-lysine (PLL; Sigma Aldrich). Neurons were cultured at 37°C in a humidified incubator with 5% CO2 and medium replaced every 5 days with fresh medium containing B27.
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bioRxiv - Cancer Biology 2019Quote: ... cells were seeded in 6-well plates and transfected the next day with 30pmol of ESR1 (Sigma-Aldrich, SASI_Hs01_00078592), ESR2 (Dharmacon ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Following washing the tissues were counterstained with 1 μg/mL DAPI (4–6-diamidino-2-phenyl indole, Sigma-Aldrich) and mounted with PromoFluor Antifade Reagent (PromoKine).
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bioRxiv - Biochemistry 2019Quote: ... catalog number G1112 and an electron coupling reagent (PMS) [CAS: 299-11-6] from Sigma (St. Louis, MO,USA).
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.
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bioRxiv - Cancer Biology 2019Quote: ... Cross-linked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
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bioRxiv - Neuroscience 2021Quote: ... band power values in each frequency band (Delta:1-4Hz; Theta:6-9.5Hz; Sigma:10-16Hz; Gamma:30-60Hz) were divided by a normalization factor that corresponds to the sum of the power across all the frequencies according to the experimental condition ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were fasted for 6 hours prior to IP injection of 2 g/kg 20% D-glucose (Sigma-Aldrich) in water with a 26G needle ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with HDM or the known AhR agonist FICZ (5,11-Dihydroindolo[3,2-b]carbazole-6-carboxaldehyde, Tocris Bioscience, Bristol, UK or Sigma-Aldrich) to activate the AhR and induce CYP1A1 transcription ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Plant Biology 2020Quote: ... and infiltrated with a solution containing 240 mM sorbitol and 6 mg.L-1 Lucifer Yellow CH dipotassium salt (LYCH, Sigma), which is used as a tracer to normalize apoplastic fluids (Derrick et al. ...