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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Biophysics 2020Quote: ... biotin binding deficient subunits equipped with a C-terminal His6-tag – “dead” – and functional subunits with C-terminal 3C protease cleaving site followed by Glu6-tag – “alive” – were expressed as inclusion bodies in E.coli BL-21 using the pET expression system (Novagen). Inclusion bodies were dissolved in 6 M Guanidine hydrochloride at 10 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or these cells stably transfected with human CB1 or CB2 receptors with 3x N-terminus haemagglutinin tags (AtT20-CB1 and AtT20-CB2respectively).17 Cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... Samples were clarified by centrifugation and incubated with anti-Myc tag antibody conjugated agarose (Millipore, Cat. no. 16-219) for 3h ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... was used for SDS-PAGE and Western blotting in Tris-buffered saline and 0.2% Tween 20 (TBST) using Strep-Tag II HRP (1:2,500; EMD Millipore) and rabbit anti-vinculin (1:1,000 ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized Bl-Eng2 open reading frame with an upstream enterokinase site and downstream c-Myc and 6X histidine tags was cloned into the SpeI and XhoI sites of the pET43.1b plasmid (Novagen); this forms a fusion protein with an upstream NusA tag ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma, Sigma-Aldrich) followed by secondary GST-purification to remove free GST tag (untagged Mcl-1 collected in flowthrough) ...
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bioRxiv - Immunology 2023Quote: ... Flag-tagged CD45 constructs in DP10.7 Jurkat T cell transductants were stained with 10ug/ml anti-Flag-tag polyclonal antibody (Sigma) for 1hr,
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Developmental Biology 2022Quote: TAG determination: 60 μl of each sample were mixed with 60 μl of Triglyceride Reagent (Cat. # T2449, Sigma-Aldrich), incubated at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cancer Biology 2021Quote: ... and Human specific Ki67 (MAB4383, 1:500, Millipore), followed by secondary antibody Envision+ anti-mouse labeled-polymer (Dako/Agilent) ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 μg/ml recombinant human insulin (Sigma). BT474 cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: F4883-500MG) were purchased from Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: T6884-100UN) and Fibronogen ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix) ...
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bioRxiv - Cell Biology 2022Quote: ... Human cells were pretreated with glycine (Millipore, 104201) at a concentration of 50 mM for at least 10 minutes before stimuli was added.
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bioRxiv - Bioengineering 2022Quote: ... human [Leu15]-gastrin I (10 nM; Millipore Sigma), N-acetyl cysteine (500 μM ...
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bioRxiv - Immunology 2022Quote: ... and 0.5% recombinant human serum albumin (Sigma Aldrich). Microfluidic droplets were generated as water-in-oil emulsions using a co-flow of aqueous phases ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Thrombin from human plasma was obtained from Millipore-Sigma (Cat ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 20% Human serum (Sigma-Aldrich, UK), and used up to passage 4.
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bioRxiv - Bioengineering 2019Quote: ... and human plasma fibronectin (Millipore Sigma, Catalog #FC010) were conjugated to fluorophores using the DyLight™ 488 Microscale Labeling Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... and 5 IU of human chorionic gonadotropin (Sigma) were injected into 3 week old B6C3F1/J female mice by intraperitoneal injection to stimulate superovulation ...
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bioRxiv - Molecular Biology 2019Quote: ... Human α-thrombin (20 nM, T6884, Sigma, USA) and human α-FXIIa (20 nM ...
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bioRxiv - Immunology 2019Quote: ... Peroxidase-labeled anti-human IgG (A8667, Sigma-Aldrich) was applied at 1:60,000 for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... and human-EGF (50 ng/ml, Sigma Aldrich), as published 60 ...
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bioRxiv - Physiology 2019Quote: ... 5 IU human chorionic gonadotropin (hCG, Millipore, 230734), after which they were immediately put in mating with fertile 2-4-month old C57Bl/6N males ...
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bioRxiv - Microbiology 2019Quote: ... Complement was obtained from lyophilized human sera (Sigma) reconstituted in sterile water as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of human AChE (Sigma, catalogue number C0663) and BuChE (Sigma ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of human CES1 (Sigma, catalogue number E0287) and CES2 (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855 ...
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bioRxiv - Bioengineering 2020Quote: ... sterile fibrinogen from human plasma (Millipore Sigma, 341576) was dissolved in sterile PBS (Corning ...
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bioRxiv - Biochemistry 2020Quote: 400 μL human male serum (Sigma; cat. #H4522) was incubated at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... with 6.3 µM human holo Tf (Sigma-Aldrich) in 0.1 M carbonate buffer (1 ml ...
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bioRxiv - Bioengineering 2021Quote: ... Polyclonal IgM isolated from human serum (Sigma Aldrich) was used as control ...