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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial pellet was resuspended in 20 ml of cold His-lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0) with protease inhibitor (Sigma-Aldrich), followed by sonication (10 x 20-second pulses) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by four washes with 200 µl of incubation buffer and incubation with 50 µl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at 24°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cerevisiae were cloned into 10×His-MBP-SUMO-nup-SNAP constructs (Figure 2A) via a pET-28a-derived vector (Novagen), and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was then transferred to a 15 mL tube and incubated with 1 mL His bead slurry (Sigma-Aldrich; P6611) for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Biochemistry 2020Quote: ... Samples corresponding to 1.4 OD600 of cells were resolved over SDS-Western blot was developed using anti-His specific antibody (Cat no. Sigma:H1029) at a dilution of 1:3000 and HRP conjugated anti-mouse secondary antibody (Anti mouse IgG-A4416 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) Tween 20) containing 5% (w/v) BSA for 1 hour and then incubated with anti-His (Sigma) or anti-GFP antibody (Clontech ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 250,000 g for 30 min and the supernatant was incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...