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Citations for Millipore Sigma :
1851 - 1900 of 3776 citations for Human MXRA8 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 3% v/v heat-inactivated Human Male AB Serum (Sigma), 2 mg/ml Human Serum Albumin (HSA ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5 IU of hCG (Human chorionic gonadotrophin, Sigma-Aldrich) with a 48-hour interval between injections ...
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bioRxiv - Bioengineering 2024Quote: IgG (from human serum; Sigma-Aldrich, St Louis, MO, USA) and fetal bovine Fetuin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Human samples were incubated with RIPA lysis buffer (Sigma Aldrich), 1x Halt™ Protease ...
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bioRxiv - Systems Biology 2023Quote: Ovulation was induced by injection of human chorionic gonadotropin (Sigma) into the dorsal lymph sac according to standard procedure166 and in accordance with approved UCSF IACUC protocols ...
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bioRxiv - Neuroscience 2024Quote: ... a human nuclear-specific antigen [(EMD Millipore; MAB1281 (1:1000)] ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 μg/ml human insulin (Sigma-Aldrich, St. Louis, MO), 100 μM ß-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ReNcell®VM human neural progenitor cell line (Sigma Aldrich) were maintained in ReNcell ®NSC Maintenance medium supplemented with 20ng/mL of epidermal growth factor ...
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bioRxiv - Bioengineering 2024Quote: ... fibrinogen (FG) from human plasma (30 mg/mL, Sigma-Aldrich), thrombin from human plasma (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: Human IGF2BP2 was cloned into p3XFLAG-myc-CMV 26 (Sigma) and pLVX-E1Fα-IRES-mCherry-Puro (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... Human organoids were additionally supplemented with hEGF ((Sigma-Aldrich, E9644) while murine cultures were supplemented with FGF2 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The single cells were incubated with human IgG (Sigma-Aldrich) at 4°C for 15 min to block nonspecific binding sites ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-human ROBO1 (HPA052968, Sigma Aldrich, St. Louis, MO); mouse monoclonal anti-human ROBO1 (MAB7118 ...
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL recombinant human insulin (Sigma Aldrich #I9278-5ml), and 1% penicillin-streptomycin (Thermo Scientific #15140122) ...
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bioRxiv - Immunology 2024Quote: ... primary antibody (anti-human lactoferrin antibody, Sigma, Cat-No. L3262), secondary antibody (Goat-Anti-Rabbit Secondary Antibody ...
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bioRxiv - Immunology 2024Quote: Antibodies were detected using goat anti-human IgG-AP (Sigma) and developed and analysed as per the mAb ELISA ...
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bioRxiv - Developmental Biology 2023Quote: ... injection of human chorionic gonadotropin (5 IU, #CG5; Sigma-Aldrich) 48 h later ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of AsCas12a nuclease in the stable HEK 293 T-REx cell line was induced by 100 ng/mL tetracycline for 24 h and assayed by Western immunoblotting using FLAG-tag specific antibodies (Sigma-Aldrich, F1804).
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bioRxiv - Immunology 2022Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30uM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60uM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and NURF-1.F isoform with FLAG tag was detected using 1:1000 Millipore ANTI-FLAG antibody (Millipore Sigma, Catalog number: F3165).
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801; HA: Roche #11867423001; VSVG: Sigma #SAB4200695) in a total volume of 500 μL 50 mM MES (pH 5.0) ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used: DYKDDDDK Tag Antibody (D6W5B rabbit mAb #14793; Cell Signaling Technology; it binds to the same epitope as Sigma’s Anti-FLAG M2 Antibody ...
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bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Plant Biology 2021Quote: ... LHCBM9 (M. Schroda, unpublished data), CF1β (Lemaire and Wollman, 1989), RPL1 (Ries et al., 2017) and the HA-tag (Sigma-Aldrich H3663). Anti-rabbit-HRP (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Molecular Biology 2019Quote: ... For Flag-tag immunoprecipitation samples were incubated with 100 µl of anti-FLAG M2 magnetic beads (#M8823, Sigma-Aldrich Chemie; Munich, Germany) for 4 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Immunology 2023Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30 µM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60 µM ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After expression was verified in cell lines by Western blot using anti Flag tag antibodies (Monoclonal ANTI-FLAG® M1, Sigma Aldrich #F3040), surface expression was verified for FPR1 using Thermo Fisher FPR1 polyclonal antibody ref number PA5-33534 ...
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bioRxiv - Biochemistry 2020Quote: ... Multiple clones were isolated and successful integration of N-terminal 3xFLAG tag was validated by western blotting with anti-FLAG-HRP (Sigma, A8592, 1:10,000).
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2020Quote: The sequences encoding mature VdAve1 and VnAve1 were cloned into pET-15b with an N-terminal His6 tag sequence (Novagen, Madison, WI, USA) (primer sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Cell Biology 2022Quote: ... Strains containing AID tag alleles were treated with final concentrations of 250 µM 3-indole acetic acid (Sigma-Aldrich, St. Louis, MO, USA) in DMSO and buffered with 50 mM potassium phosphate buffer at pH 6.2 for the 30 minutes prior to harvesting or imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...