Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... using biotynilated HA-binding protein (385911, Sigma Aldrich, USA) and biotynilated GSLII (B-1215 ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were transferred to Amersham Protran nitrocellulose membranes (Sigma) for Western blot analysis ...
-
bioRxiv - Genomics 2022Quote: ... Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010) in transfer buffer (25 mM Tris ...
-
bioRxiv - Molecular Biology 2022Quote: ... protein was transferred to a PVDF membrane (Millipore, IPFL00010). The blot was blocked for 1 hour in LI-COR Odyssey Blocking Buffer PBS (LI-COR ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein bands were transferred to PVDF membranes (Millipore). After being blocked with 5% nonfat dry milk ...
-
bioRxiv - Bioengineering 2022Quote: ... A four-molar excess of scaffold protein (MSP1E3D1, Sigma) was added to the protein-lipid mix ...
-
bioRxiv - Cell Biology 2022Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Millipore, IPFL00010) via the wet-transfer procedure ...
-
bioRxiv - Genomics 2022Quote: ... bead-bound proteins were eluted in Laemmli buffer (Sigma) and resolved on a 4–20% SDS-polyacrylamide gel (NEB) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 30 μl of protein A agarose beads (Sigma). Samples were subjected to SDS-PAGE and immunoblot analysis as described (16,20,23,82,86–87) ...
-
bioRxiv - Plant Biology 2022Quote: ... The eluted proteins were precipitated with trichloroacetic acid (Sigma) and subject to MS analyses as described previously 13.
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore, IPVH85R) at 120V for 65 min at 4C in 1x tris-glycine-methanol transfer buffer [25 mM tris base ...
-
bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... and proteins were eluted in 2X Laemmli (Sigma-Aldrich) or deglycosylated by an 1h treatment with PNGase F (NEB ...
-
bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
-
bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
-
bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
-
bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) final concentration at 95°C for 10 minutes and alkylated with iodoacetamide (Pierce,40 mM final 30 minutes) ...
-
bioRxiv - Neuroscience 2022Quote: ... 30ul of equilibrated Protein G – agarose beads (Sigma 15920010) were added to the lysates and incubated further for 3 hours.
-
bioRxiv - Neuroscience 2022Quote: ... and proteins were extracted in RIPA buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets ...
-
bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
-
bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein synthesis inhibitor cycloheximide was purchased from Sigma-Aldrich. USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A ...
-
bioRxiv - Cell Biology 2022Quote: ... the protein was concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
-
bioRxiv - Systems Biology 2022Quote: ... Protein was digested with sequencing grade trypsin (Sigma; #T6567) and sequencing grade LysC (Wako ...
-
bioRxiv - Systems Biology 2022Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 Vfor 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
-
Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to methanol activated PVDF (Millipore) membranes (350 mA for 60 minutes) ...
-
bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
-
bioRxiv - Microbiology 2024Quote: ... Protein transfer to a PVDF-FL membrane (Millipore, #05317) was achieved at a current of 2.5 mA/cm2 for one hour ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were blotted using nitrocellulose membrane (Sigma Aldrich). Rabbit monoclonal halo tag antibody was used in the (1:5000 ...
-
bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
-
bioRxiv - Biophysics 2024Quote: ... the proteins were transferred onto a PVDF membrane (Millipore). This membrane was then washed with blocking buffer (LI-COR Biosciences ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were digested with proteinase K (Cat#: P2308, Sigma), and DNA was phenol/chloroform-extracted and precipitated by 100% ethanol ...
-
bioRxiv - Molecular Biology 2024Quote: Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a PVDF membrane (Millipore), and the membrane was blocked with blocking buffer (TBST + 5% non-fat dry milk ...
-
bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich) for 30 min at 37 °C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to PVDF membranes (Merk Millipore) and probed with the appropriate indicated antibodies.
-
bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein bands were blotted onto a nylon membrane (Millipore). Blots were blocked using 5% nonfat dry milk (NFDM ...
-
bioRxiv - Physiology 2023Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 V for 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... the proteins were concentrated using Amicon Ultra-15 (Millipore) tubes before loading to a Superdex 75 column ...
-
bioRxiv - Immunology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore). Membranes were incubated overnight at 4°C with the specific primary antibodies anti-αSMA and anti-tubulin (Supplementary table 2) ...