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Citations for Millipore Sigma :
1801 - 1850 of 2422 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma) for 2 h ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: Mounted sections were incubated for 1 hour at room temperature with and blocked using blocking buffer containing (Sigma-Aldrich, #G9023) and 0.1% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue sections were stained with primary antibodies in blocking buffer (PBS + 0.3% Triton-X100 + 1% BSA (cat. # B2518, Sigma-Aldrich)) for 48 hours on a rocker at 4°C ...
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bioRxiv - Genetics 2024Quote: ... Membrane was incubated for 30 minutes in 1% skim milk for blocking and afterward incubated with primary antibody for over-night (anti-PCNA: Sc65598-santa cruz. anti-Flag:F1804-Sigma Aldrich, anti-H3 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody is diluted in blocking buffer and slides are incubated in primary antibody overnight at 4°C (ChAT, Millipore, AB144P ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were then transferred to a ChAT primary and TrkA primary antibody solution with blocking buffer (1:200 dilution goat anti-ChAT polyclonal AB143, Millipore EMD ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed cultures were cut off the membrane and washed in 1 × PBS for 10 min, incubated in blocking solution (500 µl, 1 × PBS, 0,3 % TritonX (Sigma-Aldrich), 5 % goat serum ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated for 30 minutes at room temperature in blocking buffer (0.1% PBST, 0.1% BSA (Sigma, A2153-10G)) ...
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bioRxiv - Physiology 2024Quote: ... cells were incubated with blocking buffer (PBS containing 3 % milk and 0.1 % Triton X-100) and primary antibodies against ACTN2 (1:800, Sigma, A7811) and dTyr-tub (1:1000 ...
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Sustained 3D isotropic imaging of subcellular dynamics using adaptive VCD light-field microscopy 2.0bioRxiv - Microbiology 2023Quote: ... then incubated with 0.2% Triton X-100 for 15 min and blocked with blocking buffer (3% bovine serum albumin (Sigma-Aldrich) and 0.05% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... samples were blocked with a blocking buffer (BB) containing 5% (w/v) bovine serum albumin (BSA) (Sigma-Aldrich, Taufkirchen, Germany) in 1x PBS for 1 h at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2024Quote: ... The excess of fixative was removed by washing with 1X PBS for 10 minutes and permeabilization and blocking were performed with a solution of 1% (m/v) BSA (Sigma) and 0.05% (m/v ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were washed with PBS and incubated in blocking buffer (1% BSA, 0.2% fish skin gelatin (Sigma Aldrich, G7765), 0.1% Triton X-100 in PBS ...
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bioRxiv - Biophysics 2020Quote: The lyophilized linear peptide (4.1 mg, 1 μmol) was dissolved in 100 ml refolding buffer containing 100 μmol reduced glutathione (GSH, Sigma), 10 μmol oxidized glutathione (GSSG ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... beads were washed 3X with 0.1% Tween20/TBS and 3X with TBS before elution with 3XFLAG peptide (Sigma F4799). Gel loading buffer was added to the eluates for SDS-PAGE electrophoresis and Western blotting analysis.
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bioRxiv - Cell Biology 2020Quote: ... digested sample was dried in a vacuum concentrator and reconstituted in 0.1% TFA + 1 picomole/uL Angiotensin-II peptide (Sigma). 1% of the sample was subjected to LC-MS/MS/MS analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Biochemistry 2019Quote: ... The peptide digest was dissolved in 21 μL of LC-MS grade water containing 0.1% formic acid (F0507-100ML, Sigma). The digest was filtered with a 0.65 µm pore-size micro-centrifuge filter before it was loaded to an HPLC auto-injection sample.
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... Murine BMDCs at day 8 of cultivation were incubated with 3-fold higher numbers of CD4+ CD25− T-cells (OT-II or OT-II.RAG-1-/-) and 200 ng/ml OVA peptide (323-339, grade V, Sigma) supplemented or not with our different stimuli ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Bioengineering 2019Quote: ... The gelatin and peptide solutions were prepared by dissolving in deionized water (supplied by a Millipore Milli-Q system) at 10 wt % by heating at 50°C for 30 min ...
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bioRxiv - Biophysics 2019Quote: ... The BBSome:ARL6 complex was eluted from the column with a total of 10 ml of 0.1 mg/ml Flag peptide (Sigma). Elution was performed in 5 steps ...
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bioRxiv - Cell Biology 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich, F4799)) was added and incubated with beads overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.4, 200 mM NaCl, 2 mM MgAc2, 0.25 mg/mL 3X FLAG peptide -Sigma F4799) with gentle end-over-end mixing ...
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bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by a peptide clean-up as per the zip-tip protocol (Millipore Sigma; Cat# C18 ZTC18S008). 20ul of 0.1% formic acid (in acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared in 1:1 mixture of H2O-ACN with 0.1% TFA by mixing an equal amount of peptide fractions (0.5 μl) with α-cyano-4-hydroxycinnamic acid (CCA)/2,5-dihydroxy benzoic acid (DHB) matrix (Sigma) and spotted on a stainless-steel plate and air-dried ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
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bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
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bioRxiv - Cell Biology 2021Quote: ... and kinetochores particles were eluted by gentle agitation of beads in elution buffer (Buffer H + 0.5mg/mL 3FLAG Peptide (Sigma)) for 30 min at room temperature.
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were acidified with 1% trifluoroacetic acid (TFA) and then desalted using C18 ZipTip® (Millipore cat # ZTC18 5096). Dried peptides were resuspended in 10 µL of 0.1% TFA in water ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were eluted by incubating the beads with 35 μl 100 µg/ml 3xFLAG-Peptide (F4799, Sigma Aldrich) at room temperature for 20 min or by adding Laemmli Sample Buffer (1610747 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding the selected peptides were isolated in 96-well format using the Montage Plasmid MiniprepHTS Kit (MilliPore, LSKP09604) on a MultiscreenHTS Vacuum Manifold (MilliPore ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 100 μL of buffer (50 mM Tris, 150 mM NaCl, 1mM EDTA, pH 7.4) with Flag peptide (Sigma) and rocked overnight at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2020Quote: ... cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18) ...
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bioRxiv - Physiology 2022Quote: ... 150 μl of 0.75mg/ml myelin oligodendrocyte glycoprotein 33 to 55 peptides (MOG35–55) mixed with Complete Freund’s Adjuvant (FS8810; Sigma-Aldrich) was injected subcutaneously into the tail base of 7 to 11-week-old female C57Bl/6 mice ...
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bioRxiv - Immunology 2022Quote: ... PBMC were restimulated with 1μg/mL peptide + anti-CD28 in the presence of 1μg/ml Brefeldin A (Sigma-Aldrich) for 16h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... (Ac)GCRE-ISAFLGIPFAEPPMGPRRFLPPEPKKP(Am) (BM-binder).39 All peptides were reconstituted in acidic (pH 5.5) Milli-Q water (Millipore). The concentration of free thiols in all peptides was determined using Ellman’s reagent (Sigma Aldrich).
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bioRxiv - Pathology 2019Quote: ... For MALDI-TOF/TOF/MS analysis dried peptides were dissolved in peptide resuspension solution (0.1% TFA in 5% ACN) and desalted/concentrated using C18 zip tips (Merck Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... the remaining resin was washed with 20 mL of A buffer twice and was eluted with 5mL of A buffer containing 0.8 mg/mL 3X Flag peptide (Sigma). The peptide was incubated with the resin for 20 min before elution ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1% trifluoroacetic acid (TFA) was used to extract peptides and concentrated by C18 zip-tips (Millipore,-C18, P10 size) on Xcise (Proteome Systems) ...