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Citations for Millipore Sigma :
1801 - 1850 of 2558 citations for PTH Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... cells were treated for 10 min with a solution of 0.25% Triton-X100 in blocking buffer (solution of 3% bovine serum albumin (BSA, Sigma-Aldrich) in BRB80 buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich) in tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then treated with 10 mM glycine (Fisher BP381-1) in PBS, and incubated in a blocking solution (0.1% Triton X-100, 2% fetal calf serum (Sigma F4135), and 3% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2019Quote: ... cells were incubated in blocking buffer (TBS containing 5% goat serum, 1% Bovine Serum Albumin, and 0.1% Triton-X-100 (Sigma Aldrich)) for 45 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then incubated in blocking buffer (5% v/v NDS, 0.2% v/v fish gelatin (catalog no. G7765, Sigma-Aldrich), 0.2% v/v Tween-20 in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were then probed with corresponding Li-Cor anti-mouse or anti-rabbit fluorescent secondary antibodies for one hour at room temperature at dilutions of 1/10 000 in Odyssey Blocking Buffer with 0.1% Tween-20 (Sigma-Aldrich) and 0.1% Tween ...
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bioRxiv - Cell Biology 2019Quote: ... and then washed twice with PBS following incubation in blocking solution containing 10% goat serum or 3% Bovine Serum Albumin (Sigma) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... All slices were blocked for 1hr at room temperature in blocking solution that contained 50% goat serum (Millipore S26-100ML) and 0.01% Triton X-100 (Sigma T9284 ...
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bioRxiv - Plant Biology 2021Quote: ... the pellets were resuspended with 100 μl of blocking solution (1X PBS, 1% BSA and 0.1% saponin) containing 50 μg/ml of TRITC-phalloidin (Sigma-Aldrich) and 2.5 μg/ml of 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were blocked and permeabilized with blocking buffer (5% (w/v) BSA (Jackson Immuno Research) and 0.5% (v/v) TritonX-100 (Sigma-Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The sections were washed with PBS and blocked in Blocking buffer (PBS with 0.3% Triton X-100 (T8787, Sigma Aldrich) and 5% normal goat serum (G9023 ...
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bioRxiv - Microbiology 2019Quote: ... the plate was washed and incubated with Streptavidin-Peroxidase diluted in blocking buffer (Sigma-Aldrich, 1 mg/mL, 1:5000) during 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Immunology 2020Quote: ... Plates were then blocked with ELISA blocking buffer containing 2% w/v bovine serum albumin in PBS with 0.1% v/v Tween20 (PBS-T) (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Then slices were washed with 0.2% Tween-20 and incubated with a blocking buffer consisting of 5% NGS (Sigma-Aldrich) and monoclonal mouse anti-ankyrinG antibody (1:500 ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS (pH 7.4) and incubated with blocking buffer (2% bovine serum albumin (Sigma-Aldrich) in PBS-Tween 20 (0.05% ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then treated with 5% BSA blocking solution for 1 h and the incubated with mouse monoclonal anti-HA (Sigma) antibody and rabbit anti-Flag antibody (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... and non-specific binding sites were blocked by immersing the membranes in blocking buffer containing 5% non-fat milk (70166, Sigma) in TBST buffer for 1 h on a shaker at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... For ZO-1 images cultures were fixed in 4% PFA for 20 min at room temperature, followed by permeabilization (Permeabilization Buffer set, Ebioscience) and blocking with 2% BSA solution (Sigma). Cells were stained with anti-ZO-1 mouse mAb (Thermo-Fischer ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were washed with blocking buffer (4X-SSC/0.1% Triton X-100/4% BSA (Bovine serum albumin; Sigma, catalog #A7906)) at RT for 30 mins ...
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... transferred across three drops of filtered PBS and then incubated on drops of filtered blocking solution containing 2% BSA (Sigma) in PBS for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Microbiology 2021Quote: Single-nucleotide EdU-labelled slides were incubated in blocking solution (1% BSA in PBS) for 30 mins and then 1:100 anti-centrin antibody (clone 20H5, Millipore) in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Biochemistry 2021Quote: ... After blocking the membrane with 2 % non-fat dry milk dissolved in PBS + 0.1 % (v/v) tween-20 (PBST; Sigma-Aldrich), membranes were incubated with primary antibodies for either 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The sections were then incubated in the blocking solution (PBS containing 10% donkey serum (cat no: S30-100ml, Millipore Sigma), 2% BSA (cat no ...
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bioRxiv - Neuroscience 2021Quote: ... Inner ear was firstly immersed in blocking solution (4% BSA (V900933, VETEC) in 0.5% PBS-Triton X-100 (T8787, Sigma-Aldrich)) for 1 day ...
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bioRxiv - Neuroscience 2021Quote: ... and the non-specific binding was blocked using a blocking buffer containing 3% bovine serum albumin (BSA) (Sigma-Aldrich, #A9647) in PBST (137 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed 3 x 5 min with 3% BSA in PBS-T and incubated with the following DNA dye and secondary antibody dilutions in blocking solution for 3 hours at room temperature: Hoechst 33342 (0.2 mM, Sigma-Aldrich), Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Neuroscience 2020Quote: ... They were blocked by incubating with Duolink blocking solution according to the manufacturer’s indications (Duolink® in situ PLA kit, Sigma). Cells were incubated with rabbit polyclonal anti-HA.11 and mouse monoclonal IgG1k anti-GFP antibodies at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... then permeabilized and blocked for non-specific binding in blocking buffer containing 3% normal horse serum and 0.25% Triton X-100 (Sigma X100) in a humidified chamber for 1 hr at room temperature8 ...
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bioRxiv - Genomics 2021Quote: Sections were washed in 0.1M TBS and then non-specific binding site blocking and cell permeabilization was performed with blocking buffer containing 3% normal horse serum and 0.25% Triton X-100 (Sigma X100). Sections were incubated with primary antibody (see list below ...
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bioRxiv - Neuroscience 2022Quote: ... permeabilization and blocking of non-specific binding with 0.2 % Triton-X and 3 % bovine serum albumin (BSA, A7906, Sigma Aldrich), samples were incubated overnight at 4 °C with a solution of PBS containing 0.2 % Triton-X ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS three times and then incubated in blocking solution (PBS supplemented with 0.1% Triton-X-100 [X100, Sigma-Aldrich] and 10% normal donkey serum [017-000-121 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Blocking of unspecific antigens was carried out by incubation in 3% bovine serum albumin (BSA; Sigma-Aldrich; dissolved in PBST) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated (30 min at room temperature) in blocking buffer with (permeabilized cells) or without (non-permeabilized cells) 0.05% (w:v) saponin (Sigma-Aldrich, 558255), 0.5% (w:v ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 3 times in PBS (10 minutes per wash) and then incubated for 1 hour in blocking solution containing 3% normal donkey serum (NDS, SC30-100ML, Merk Millipore) and 0.2% Triton X-100 (648464 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated overnight in primary antibodies targeting LC3 (1:2000) and p62 (1:2000) and aSyn (1:500) diluted in blocking solution with 0.1% Tween-20 (Sigma-Aldrich). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubation of the tissue in blocking solution (5% normal goat serum (Sigma, G6767), 1% blocking reagent (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex antibody cocktails were made in a whole-mount blocking solution consisting of 5% heat activated normal donkey serum (Sigma Millipore), 20% Dimethyl sulfoxide (Sigma Millipore) ...