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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and 1μL of 2μg/μL plasmid with 0.1% Fast Green (Sigma) was injected by hand into the lateral ventricle using a Picospritzer II (Parker) ...
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bioRxiv - Microbiology 2019Quote: ... digested with NdeI and BamHI and inserted into plasmid pET11b (Novagen). For expression of His-tagged proteins (GapDH ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli cultures using Sigma GenElute™ Plasmid Miniprep kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... plasmids were transformed into BL21-Rosetta 2 (DE3)-competent cells (Millipore). The E ...
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bioRxiv - Biochemistry 2019Quote: ... and ligated into digested pET28a(+) plasmid (#69864-3, Novagen, Merck Biosciences). QIAquick® PCR Purification Kits (QIAGEN ...
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bioRxiv - Immunology 2021Quote: ... the kanamycin (km) resistance cassette was amplified from plasmid pET26b (Novagen) using primers k1 and k2 ...
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bioRxiv - Bioengineering 2020Quote: ... and assembled into the pCDF-1b plasmid (ColDF13 ori, Millipore, US) replacing the MCS region by Gibson Assembly ...
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bioRxiv - Cancer Biology 2020Quote: ... pETDuet-Twist-E47 plasmids were transformed into Rosetta2(DE3)-pLysS (Millipore) bacteria ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids were transformed into Rosetta2 (DE3) competent cells (Novagen, 71397). The recombinant proteins were purified using SBP-tag and StrepTactin Sepharose ...
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bioRxiv - Microbiology 2019Quote: ... and cloned into a pSF-CAG-KAN plasmid (Sigma-Aldrich, Merck). These were co-transfected into DF-1 cells with Lipofectamine™ 2000 and the supernatant containing virus was passaged onto additional DF-1 cells to generate viral stocks ...
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bioRxiv - Biophysics 2021Quote: ... and isolated using the GenElute Plasmid Miniprep kit (Sigma, Cat# PLN350). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... the plasmids were transfected with the FuGENE HD Transfection Reagent (Sigma). After transfection ...
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bioRxiv - Biochemistry 2022Quote: ... all expression plasmids were transformed into BL21DE3 codon plus cells (Novagen) and protein expression induced by addition of 0.1mM isopropyl-β-D-thiogalactoside and overnight shaking at 16°C ...
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bioRxiv - Biochemistry 2022Quote: ... retromer plasmids were transformed into BL21(DE3) Rosetta2 pLysS cells (Millipore). Cells were grown to an OD600 between 0.8-1.0 and induced for 16-20 hours at 22°C with 0.4 mM IPTG ...
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bioRxiv - Biochemistry 2021Quote: ... The expression plasmids pET15b and pMAL-c2X were obtained from Novagen and New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... the appropriate plasmids were transformed into Rosetta2(DE3)pLysS cells (Novagen) and transformants on Luria Broth (LB ...
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bioRxiv - Systems Biology 2020Quote: ... We amplified attB-TurboGFP off of the SHC003 plasmid (Sigma SHC003).
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmid pET-Himar-dCas9 was electroporated into competent Rosetta2 cells (Novagen); transformants were selected on chlor (34 ug/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid DNA was transformed into Rosetta2 DE3 cells (Novagen Inc.) and overexpressed in 4 L of Terrific Broth media by supplementing 2% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... Gene expression from the plasmid was induced by adding rhamnose (Sigma) to a final concentration of 0.2% (w/v) ...
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bioRxiv - Microbiology 2021Quote: ... coli was done using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich) or the Monarch Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Biochemistry 2020Quote: ... The plasmid was transformed into Rosetta 2(DE3)pLysS cells (Novagen).
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bioRxiv - Cell Biology 2019Quote: ... retromer plasmids were transformed into BL21(DE3) Rosetta2 pLysS cells (Millipore). Cells were grown to OD600 between 0.8-1.0 and induced for 16-20 hours at 22°C with 0.4 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... by PCR and inserted into bacterial expression plasmid pET23a (Novagen, USA) in order to construct either pET-hGFAT2 with and without HisTag ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We purified the vector with the GeneEluteTM Plasmid Miniprep kit (Sigma), and prepared the injection mix at 300 ng/μl vector concentration diluted with injection buffer (5 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... NK92 cells were transduced with lentivirus vector pLKO-(Plasmid TRCN0000039984; Sigma), pLKO.1-puro eGFP shRNA control (Plasmid SHC005 ...
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bioRxiv - Genetics 2020Quote: The plasmids were transformed into BL21(DE3) RIL cells (Novagen Inc). When the cell density reached an optical density at 600 nm (OD600 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the CRISPR-lenti lentiviral vector Non-directed control plasmid (Sigma-Aldrich) was used ...
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bioRxiv - Biophysics 2022Quote: The expression plasmid was transformed into Rosetta 2 (DE3) cells (Novagen). 20 mL LB with 38 μg/mL chloramphenicol and 100 μg/mL carbenicillin was inoculated with a single Rosetta 2 (DE3 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was prepared using the Midi-Prep kit (Sigma-Aldrich) and preparations were analyzed after digestion with endonucleases by electrophoresis in 0.8% agarose gel in TAE buffer (40 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... and 5 ng of pEF-Slc46 expression plasmid using GeneJuice (Millipore) for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... host genome and plasmid DNA were digested with DNase I (Sigma) before releasing the viral DNA from the particles ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA plasmids were complexed with polyethyleneimine (PEI; #408727, Sigma-Aldrich, USA) for 15 min and added dropwise to the cells ...
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bioRxiv - Biochemistry 2023Quote: Lysozyme gene was amplified from the pLysS plasmid (Millipore Sigma-Novagen) with the oligonucleotide primers Fw-lys-NdeI (CCCATATGGCTCGTGTACAGTTTAAACAACGTG ...
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bioRxiv - Biochemistry 2023Quote: Lysozyme gene was amplified from the pLysS plasmid (Millipore Sigma-Novagen) with the oligonucleotide primers Fw-lys-NdeI (CCCATATGGCTCGTGTACAGTTTAAACAACGTG ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was transformed into Rosetta (DE3) pLys (Novagen, 70956) and a 100 ml overnight culture was grown overnight at 37°C in LB medium supplemented with kanamycin (50 ug/ml ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were transformed into chemically competent Escherichia coli Rosetta cells (Novagen) and grown at 37°C in Super Broth with kanamycin (30mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was electroporated into Rosetta™ 2 cells (Millipore Sigma) for protein expression ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid backbones were amplified from the commercially available pCOLADuet (EMD Millipore) expression vector via PCR followed by digestion with the restriction enzyme DpnI ...
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bioRxiv - Systems Biology 2023Quote: The NSP10-NSP16 co-expression plasmid (cloned into pETDuet-1, Novagen) was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... and other plasmid construction are located in Table S2 (Sigma Aldrich). Bacterial strains and plasmids used or generated in this study are described in Table S1.
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bioRxiv - Biochemistry 2023Quote: ... coli DH5a into pACYCDuet-1 bicistronic plasmid (Sigma-Aldrich, Burlington, MA), and the resulting co-expression plasmid was introduced to E coli ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid and 130 μL 1 μg / mL polyethylenimine (Sigma-Aldrich) in 450 μL Opti-MEM media (Lonza) ...
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bioRxiv - Biophysics 2023Quote: ... was cloned using Gibson assembly 40 into plasmid pET15b (Novagen/Merck), which was digested with NcoI ...
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bioRxiv - Genetics 2023Quote: ... The plasmids were transformed into Rosetta(DE3)pLysS cells (Sigma-Aldrich). Protein expression was induced with isopropyl b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... and 500 μg of pXX6 plasmid mixed with polyethylenimine (Sigma-Aldrich). The UPV-UAB generated a pAAV plasmid containing the ITRs of the AAV2 genome ...
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bioRxiv - Molecular Biology 2023Quote: ... The respective plasmid was transformed into BL21 (DE3) competent cells (Novagen), grown in LB broth or M9 medium supplemented with 100 mg/ml ampicillin and induced for expression with a final concentration of 0.5 mM isopropyl-D-1-thiogalactopyranoside at an optical density (600 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid was transformed into Rosetta2(DE3) pLysS competent cells (EMD Millipore) and plated on LB agar plates containing 100μg/mL ampicillin and 34μg/mL chloramphenicol ...
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bioRxiv - Genetics 2023Quote: Plasmid transfection was carried out with XtremeGENE HP (Millipore Sigma, 6366244001) according to manufacturer protocol at a reagent/DNA ratio of 2:1 in serum free media ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse anti-Gapdh (clone GAPDH-71.1, cat# G8795) and mouse anti-MBNL1 (clone 3A4) were from Sigma. Rabbit anti-Tubulin (cat# 2148) ...