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Citations for Millipore Sigma :
1801 - 1850 of 6718 citations for MXRA8 protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved into 5 mL of BugBuster protein extraction reagent (Sigma). Using a sterile loop ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from cell pellets resuspended in RIPA buffer (Sigma) containing cOmpleteTM Mini Protease Inhibitor Cocktail (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were then concentrated using Amicon 3-kDa concentrators (Millipore), and the purity of each protein was assessed by SDS-PAGE and further confirmed by Western blotting using commercial antibodies against the strep tag (Biobharati Life Sciences ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF membrane (Millipore, catalog #IPVH00010) at 100V for 30 min in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma) for 5 min at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... Protein was concentrated with Amicon® Ultracel ® 3K filters (Millipore) for immunization of rabbits by Covance (Denver ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were detected with anti–HA–peroxidase (Sigma, clone 3F10), anti–FLAG (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... was immobilised on MagnaChIP™ Protein A magnetic beads (Sigma-Aldrich). Both the wildtype as well as the med25-1 mutant were used for the analysis under normoxic and hypoxic conditions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to an Immobilon-FL membrane (Sigma Aldrich, USA) using a humid transfer device and 350 mA ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 10 μg from each sample was separated in 10% SDS-PAGE gels ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 35 μg of protein were electrophoretically separated using 8% SDS-PAGE and transferred to PDVF membranes for 2.5 h at 200 mA per membrane using a semi-dry system at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Protein content was determined using a Bradford assay (Sigma-Aldrich, USA).
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA) overnight ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After transferring the proteins to PVDF membranes (Millipore, Etobicoke, ON, Canada) as described ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto PVDF membranes (Millipore, Billerica, MA, USA), the membranes were quenched and proteins were detected using specific antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... proteins were transferred to PVDF membranes and blocked with 5% BSA (Sigma) followed by probing with mAb MoPn-40 (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were transferred to an Immobilon P membrane (EMD Millipore, IPVH00010) at 25°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cancer Biology 2021Quote: ... separated protein bands were transferred into polyvinylidene fluoride membranes (Millipore, Cat. #IPVH00010). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was wet transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Millipore) in Criterion blotter cells (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 20µl of DBX-washed Magna ChIP Protein W+G magnetic beads (Millipore) were added to each sample and rotated for 1hr at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were purified with α-FLAG M2 affinity gel (Sigma-Aldrich) as described for co-IP ...