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Citations for Millipore Sigma :
1801 - 1850 of 4166 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). qPCR was performed as described above ...
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bioRxiv - Plant Biology 2023Quote: ATP was measured using the Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit (Sigma-Aldrich) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The FastStart Essential DNA Green Master kit (Sigma-Aldrich Corp. St. Louis, MO, USA) was used to carry out qPCR in a Roche LightCycler® 96 ...
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bioRxiv - Cell Biology 2023Quote: ... Periodic acid-Schiff staining was performed using a PAS staining kit (Sigma-Aldrich, 101646) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slides were stained according to the kit protocol and mounted using DPX (Sigma-Aldrich). Brightfield Z-stacks of dendrites from ventral hippocampal CA1 pyramidal neurons were taken at 60x magnification and analysed using ImageJ (https://imagej.nih.gov/ij/index.html).
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bioRxiv - Immunology 2024Quote: ... Then proliferation was assessed with a Cell Counting Kit-8 (Sigma-Aldrich, Cat#: 96992) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Cell Biology 2023Quote: ... Cell lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma Aldrich). Caco2 colon epithelial cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were performed using the manufacturer supplied instructions in the kit (Sigma Aldrich). PLA-fluorescent labelled cells were passed through a 100µm strainer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proximity ligation was then performed using manufacturer instructions supplied with the kit (Sigma Aldrich). The samples were stained with DAPI and images were acquired using either Olympus Ix71 inverted fluorescence tissue microscope (single experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit protocol (Sigma-R4775) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... RNA input was poly (A) purified using the GenElute mRNA kit (Sigma, #MRN 70). The remaining cleared lysate was subjected to immunoprecipitation with 10 μg of YTHDF2 antibody under constant rotation at 4° C for 4 h ...
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bioRxiv - Plant Biology 2024Quote: ... We extracted total RNA from the samples using Spectrum Plant Total RNA kit (Sigma). RNA samples were treated with DNase I during RNA extraction ...
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bioRxiv - Physiology 2023Quote: ... plasma total bilirubin concentration was measured using a bilirubin assay kit (Sigma-Aldrich, #MAK126). Results are expressed in milligrams per deciliter (mg/dL).
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bioRxiv - Molecular Biology 2023Quote: ... The 5’-pGEM-T construct was then isolated with GenEluteTM Plasmid miniprep kit (Sigma) and digested with 0.5 µl (10 U ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Masson’s trichrome staining was performed with a commercially available kit (Cat#: HT15-1KT, Sigma) per vendor recommendation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated using a GeneElute mammalian genomic DNA miniprep kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proximity ligation assays (PLAs) were performed with a Duolink in-situ PLA kit (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Neuropeptide Y levels were measured using commercially available ELISA kits (EMD Millipore, EZHNPY-25K).
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with the corresponding MINUS/PLUS PLA probes (Sigma DUOlink PLA kit) for 1 hour at 37°C in a warm foil-covered box ...
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bioRxiv - Biochemistry 2024Quote: ... The bone slices were fixed and stained with a TRAP staining kit (Sigma-Aldrich) to virtualize multinucleated osteoclasts on the bone slices ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was purified using GenElute Single Cell RNA Purification Kit (Sigma-Aldrich RNB300), treated with DNase I on column (Sigma-DNASE70-1SET) ...
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bioRxiv - Bioengineering 2024Quote: Masson’s trichrome staining was performed using the trichrome staining kit (Sigma-Aldrich Sweden AB) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: Plasma magnesium levels were determined using a commercial magnesium assay kit (Sigma- Aldrich MAK026) from individuals from the same study as in (Collins et al ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated with a TransPlex whole-transcriptome amplification kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... IL-22) were determined using the Mouse Th17 Magnetic Bead Panel Milliplex MAP kit (Millipore). Samples were not diluted and assay was performed according to the manufacturer’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hmga2-/- and doxycycline inducible MEF was extracted using the GenElute DNA Miniprep kit (Sigma-Aldrich) according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were used to assay PP2A phosphatase activity by a standard kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total cholesterol content of cellular samples was determined using Cholesterol Quantification Kit (MAK043, Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells using a GenElute Mammalian Total RNA Miniprep kit (Sigma) and reverse transcribed with SuperScript IV reverse transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... CK activity in the serum was measured using Creatine Kinase Activity Assay Kit (MAK116, Sigma).
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bioRxiv - Developmental Biology 2020Quote: ... Indexing and amplification was done using the KAPA HiFi HotStart PCR kit (Sigma-Aldrich, KK1508) with Index oligonucleotides (sequences were adapted from Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG) Assay Kit (Sigma-Aldrich, USA). Paired GBM cell lines were also treated with hit inhibitors individually and in combination ...
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bioRxiv - Immunology 2022Quote: Chemicals reagents used to prepare buffers and BCA Assay kit were purchased from Sigma Aldrich, UK and Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Blood MeS markers were then quantified using an ELISA kit (insulin: EZRMI-13K, Millipore; glucose) and colorimetric assays (triglyceride ...
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bioRxiv - Biophysics 2020Quote: ... Thrombin cleavage of the affinity tag was performed using the Sigma Thrombin CleanCleave Kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was purified using the High Pure PCR Product Purification Kit (11732676001 Sigma-Aldrich). Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571) and the Reverse transcription PCR kit from Millipore Sigma (Product No. HSRT100), with specific primers were designed for each target gene (Supplementary Table 3) ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Release of IL-2 into supernatant was measured using a commercial ELISA kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... The samples were processed further using a Duolink® In Situ Red kit (Sigma-Aldrich), following the instructions of the manufacturer ...