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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... High-purity nucleotides and chemicals were bought from Sigma Aldrich, and HPLC-quality acetonitrile was from Serva.
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bioRxiv - Cell Biology 2022Quote: ... High Sensitivity Triglyceride Fluorometric Assay Kit from Sigma (MAK264-1KT) and Phospholipid quantification Assay Kit from Sigma (CS0001-1KT)).
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bioRxiv - Cell Biology 2019Quote: ... 2 mM L-glutamine in high glucose DMEM (Sigma, D6546)) at an MOI of 0.50 by spinoculation at 1000 xg for 30 min at 20°C ...
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bioRxiv - Immunology 2021Quote: ... high salt PBS (PBS pH 7.4, 2 M NaCl [Sigma]), and denaturing urea buffer (50 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... and HEK293T cells were cultured in DMEM-high glucose (Sigma) supplemented with 10% FBS (Gemini Bio) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Cells were maintained in DMEM high glucose media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2021Quote: ... high-glucose Dulbecco’s modified eagle medium (DMEM; Sigma-Aldrich, Canada) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: All high purity chemical standards were purchased from Sigma-Aldrich Germany ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in high glucose DMEM (Sigma-Aldrich, D5671) that was supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... purified high titer lysates were treated with Benzonase Nuclease (Sigma) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... NS-Fibroblasts were cultured in DMEM (high glucose, SIGMA #D5796) at 32°C under humanified atmosphere containing 5% CO2 and 5% O2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were cultured in DMEM with high glucose (Sigma-Aldrich), supplemented with 10% FCS ...
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bioRxiv - Immunology 2020Quote: ... High Salt Immune Complex Wash Buffer (Millipore, Cat #20-155) was added to the pellet and the sample was spun for five minutes at 4°C then pelleted ...
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bioRxiv - Molecular Biology 2020Quote: ... High fidelity KOD Hot Start polymerase was purchased from Novagen/EMD Millipore (Darmstadt ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were cultured in high-glucose DMEM (Sigma-Aldrich) supplemented with 10% FBS (fetal bovine serum).
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bioRxiv - Systems Biology 2021Quote: High-performance liquid chromatography (HPLC)-grade ammonium acetate from Sigma, acetonitrile ...
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bioRxiv - Genetics 2019Quote: ... incubated with 2 ml high sensititivity ECL detection solution (Millipore), and blots were visualized on a GeneGnome chemiluminsescence imaging system.
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bioRxiv - Microbiology 2019Quote: ... Cell lines were grown in high-glucose DMEM (Sigma: D6429) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Glial culture media contained high glucose DMEM (Sigma-Aldrich, 51441C), 10% heat inactivated fetal bovine serum (ATCC ...