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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Protein A Sepharose® beads (Sigma) were coated with PBS + 0.3% BSA ...
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bioRxiv - Genetics 2023Quote: ... acid-washed glass beads (Sigma, #G8772) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... or protein A-agarose beads (Sigma) for 2-3 hours at 4℃ ...
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bioRxiv - Developmental Biology 2024Quote: ... EZView protein G affinity beads (Sigma) were washed with lysis buffer A 3x times ...
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bioRxiv - Cell Biology 2024Quote: ... Acid-washed glass beads (Sigma; G8772) were added to the samples and cells were lysed using a FastPrep-24 5G bead beating grinder (6 times shaking at 100 V for 30’’ ...
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bioRxiv - Cell Biology 2024Quote: ... Acid-washed glass beads (Sigma, G8772) were added to the samples and cells were lysed using a FastPrep-24 5G bead beating grinder (6 times shaking at 100 V for 30’’ ...
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bioRxiv - Developmental Biology 2024Quote: ... EZView protein G affinity beads (Sigma) were washed with lysis buffer A 3x ...
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bioRxiv - Cancer Biology 2024Quote: ... or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: CDC20 cDNA was cloned into the multiple cloning site of the expression vector p3XFLAG-myc-CMV-26 (Sigma). Site-directed mutagenesis was used to generate the CDC20 mutants of interest ...
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were briefly sonicated using a M220 focused ultrasonicator and incubated with Myc antibody (1:500; Sigma, C3956) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 16,000 g for 10 min at 4 °C and then incubated with 10 µl anti-FLAG M2-Sepharose beads (Sigma) for 60 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... One mg of protein in a final volume of 500 µl was incubated with 20 µl of anti-flag agarose beads (Sigma) pre-washed with IP buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Flag-KIF4A complexes were isolated by 2h incubation at 4°C with 15µl anti-Flag M2-agarose beads (Sigma-Aldrich). Beads were washed twice with 1ml lysis buffer and twice with 1ml wash buffer (20mM Tris-HCl pH7.4 ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by French press and the lysate was incubated with anti-FLAG M2-agarose beads from SIGMA. Bound substrates were then eluted from the beads with 100 mM Glycine pH 2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... After sonication (Bioruptor, 10 min, cycle 30/30 sec) and centrifugation (4°C, 15 min) anti-FLAG agarose beads (M2, Sigma) were added to the supernatant and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysates were incubated for 2 hours at 4 °C with 30 µl of precleared slurry EZ view Red Anti-FLAG M2 Affinity Gel beads (Sigma). Beads were washed three times with RIPA buffer and resuspended in 50 µl of Laemmli buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell lysates were diluted 10-fold in Tris-HCl buffer and incubated with anti-FLAG M2 agarose beads (Sigma) for 4 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared 48 h later and were incubated with 50 μl of a 1X PBS-washed anti-FLAG bead slurry (Sigma) overnight at 4 °C with gentle rocking ...
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bioRxiv - Microbiology 2020Quote: ... The remaining crude lydate was cleared by centrifugation and incubated with 40 μl prepared Anti-FLAG M2 beads (Sigma-Aldridge) with rotation at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondrial lysates were cleared by centrifugation at 20,000× g at 4°C and transferred to pre-equilibrated anti-FLAG M2 agarose beads (Sigma-Aldrich) for FLAG immunoprecipitation or onto protein A-Sepharose (PAS ...
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bioRxiv - Cell Biology 2021Quote: ... The whole cell extracts were cleared by centrifugation at 16000g for 20 minutes and the supernatants were subjected to immunoprecipitation with EZView anti-flag beads (Sigma) for 4 hours at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested DNA was then incubated with 50 µl of protein A/G agarose beads and 5 µl anti-S9.6 (Millipore, MABE1095) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were centrifuged at 21,130g for 15 minutes at 4°C and supernatants were added to 50 μl of M2 anti-FLAG-agarose beads (Sigma). Beads were washed 4 times with NET-2 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The purification of 3XFLAG-tagged 26S proteasome was carried out by affinity purification using M2 anti-FLAG beads (Sigma A2220). After incubation for 1.5 hrs at 4°C the proteasome was eluted with FLAG peptide ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ul of the resulting supernatant was used as input and the rest was incubated with EZview anti-FLAG M2 beads (F2426, Sigma) for 1-2 h at 4°C on an end-over-end shaker ...
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bioRxiv - Cell Biology 2022Quote: ... 10 % of each lysate was collected (input) and the remaining lysate was incubated with Anti-V5 Agarose Affinity Gel beads (Sigma) or Anti-FLAG M2 magnetic beads (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... 1/20 of each sample was saved as an input control and the remaining lysate was incubated with anti-flag M2 agarose beads (Sigma) over night at 4°C on a turning wheel ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then eluted off the anti-Flag beads using competitive 500 µg/mL 3xFlag peptide (Sigma-Aldrich, F4799-4mg) elution in 150 µL lysis buffer by rotation for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μL of beads were washed with PBS and incubated with 6 μg of anti-FLAG antibody (Sigma-Aldrich, F1804) in PBS + 0.5% BSA at 4°C for 3 h with gentle rocking ...
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bioRxiv - Pathology 2019Quote: ... the cells were lysed in RIPA buffer and the clarified supernatant was subjected to immunoprecipitation by anti-flag M2 beads (Sigma) for 2 hr ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were spun for 5 seconds in a microfuge and 950 μl of supernatant was transferred to a new tube and incubated with agarose beads-conjugated to anti-Flag antibody (1:100; Sigma) for two hours at 4°C ...
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bioRxiv - Genetics 2020Quote: ... and were then used for pull-downs with the respective antibody-conjugated beads: either the anti-FLAG M2-FLAG affinity gel (Sigma) (20 μl per IP ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 300 mL of centrifuged cell lysate were split into two batches and immobilized four times on two gravity flow columns - one containing 0.5 mL Streptactin XT high capacity beads (IBA) and one containing 0.5 mL anti-FLAG M2 affinity resin (Sigma-Aldrich) (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... 700 µg of protein was subjected to immunoprecipitation using 30 µl of Anti-FLAG M2 affinity beads (A2220, Sigma-Aldrich). The beads were incubated with rotation for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... rPAI-1 was further purified by binding to anti-FLAG agarose beads and elution with 3X FLAG peptide (Millipore Sigma). rPAI-1 was quantified spectrophotometrically (ε280nm = 43,000 M−1 cm−1) ...
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bioRxiv - Cancer Biology 2022Quote: ΔNp73α complexes were immunoprecipitated from each fraction using 30 μl of slurry anti- HA-agarose beads (Sigma-Aldrich, ref. A2095) or 20 μl of pre-coupled anti-E2F4 or anti-p73 Sepharose beads (dry bead volume) ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysates were then spun at 20,000×g for 30 min and supernatants were incubated with 10 µl prewashed anti-Flag agarose beads (Sigma, A2220) at 4°C for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Thereupon the samples were transferred to 1.5 ml tubes containing 100 μl of Anti-FG M2 agarose beads (Millipore-Sigma) and incubated at 25° C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were spun at 30,000 g for 10 min at 4°C and the cleared lysate was incubated with 40 µl anti-FLAG agarose bead slurry (Sigma) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was achieved by incubating cell lysates containing 1 mg of protein overnight with anti-HA agarose beads (Sigma-Aldrich), Myc-agarose beads (Santa cruz biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...