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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Neuroscience 2021Quote: ... the mice were given an intraperitoneal injection of 10 mg kg-1 of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) dissolved in a 1:4 mixture of castor oil (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... tadpoles were euthanized using MS-222 (1 g/L) and fixed in 4% paraformaldehyde (Sigma - CAS 30525-89-4) for 3 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... and the sample was incubated for 1.5h rotating at 4°C before the addition of 1/4 volume of 3.06% cycloamylose (Sigma-Aldrich). The sample was incubated overnight rotating at 4°C ...
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...
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bioRxiv - Genomics 2021Quote: ... Samples were homogenized by adding one half of the TRI-Reagent (Sigma-Aldrich, 1 ml per 100 mg) to each sample following sample disruption by using the Precellys 24 Tissue Homogenizer (Bertin ...
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bioRxiv - Bioengineering 2022Quote: ... Twelve-millimeter round glass coverslips were floated on one drop of 1% poly-L-lysine (Sigma Aldrich P1399) each and allowed to stand for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with one of the following primary antibodies: anti-cyclin G2 (1:500, HPA034684, Sigma); Flag (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 1×106 cells per were re-plated onto one well of a poly-L-ornithine (PLO, Sigma-Aldrich) and 15 µg/mL Laminin-coated (R&D ...
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bioRxiv - Cell Biology 2020Quote: ... Experimental mice were injected subcutaneously with one dose of CL316,243 (CL, Sigma-Aldrich, 1 mg/kg body weight) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... One flask of cells was treated with 1-2 μM (98, 99) TAT-Cre Recombinase (EMD Millipore, #SCR508) while the other was treated with DPBS alone ...
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bioRxiv - Cell Biology 2023Quote: ... One well was plated at 225k cells/well supplemented with 1 µM Cdk4/6i (PD-0332991, Sigma, #PZ0199) to synchronize cells at the G1/S transition ...
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bioRxiv - Bioengineering 2023Quote: ... Diabetes was induced through a one-time intraperitoneal injection of 110 mg kg−1 streptozotocin (Step, Sigma Aldrich) dissolved in citrate buffer (pH 4.5 ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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The RIFF: an automated environment for studying the neural basis of auditory-guided complex behaviorbioRxiv - Neuroscience 2021Quote: ... and 1:1 mixture of 4% sucrose and 0.1 % saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Cell Biology 2019Quote: ... for 30 minutes and incubated overnight at 4°C with anti-TAT1 (1:250) and/or anti-GFP (1:500, Sigma) antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Frozen brain tissue was ground with a motorized pestle for 30 s in 100 µl of chilled (4:1) analytical grade methanol:ddH2O with 1 mM norluecine (Sigma Aldrich) standard ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma) in PBS-T containing 2% normal donkey serum ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636 ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... Universal matrix composition: mixture of 1:1 of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (2,5-DHB) (Sigma-Aldrich solubilized in 78:22 MeCN in H2O + 0.1% TFA.
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bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Primary antibodies were incubated overnight at 4°C and included Col2 (1/100, Thermo) and Sox9 (1/1000, EMD Millipore). Negative controls included a mouse IgG (2µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were cultured in 4 mL CgXII medium containing 13C-1-glucose or 13C-1-fructose (Sigma-Aldrich, Taufkirchen, Germany) as sole carbon sources ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... afterwards with secondary antibody mix for 1 day overnight at 4°C and finally with DAPI (1:10000, Sigma-Aldrich) for 8 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Phage 80α stock was diluted in phage buffer (50 mM Tris-HCl pH 7.8, 1 mM MgSO4, 4 mM CaCl2 and 1 g/L gelatin; Sigma–Aldrich), and added to the master mix to reach the desired starting concentration in plaque forming units per mL (pfu/mL) ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed 4 times with PBS and resuspended in PBS containing 1% BSA and DAPI (1 μg/mL; Sigma) as a nuclear stain ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...