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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Systems Biology 2024Quote: ... 60µg of total protein per sample and 5µg recombinant Tau (rTau) (2N4RTau and 1N3R Tau) were digested with either Trypsin Protease (Pierce) or LysargiNase (EMD Millipore) at 1:50 w/w overnight at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... 9 mg of protein were mixed with 20 µL of S-protein Agarose beads (#69704, Novagen), that had been previously washed in extraction buffer with 1% (w/v ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
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bioRxiv - Biophysics 2019Quote: ... or fibrinogen (0.02 mg/mL, from human plasma, Sigma # F-3879) overnight at 4 °C unless otherwise stated.
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bioRxiv - Systems Biology 2019Quote: ... with standard curves of purified human RBP4 (72) or TTR (Sigma) protein on each blot (57) ...
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bioRxiv - Biochemistry 2019Quote: ... Human serum albumin (catalog # A3782) was from Sigma-Aldrich (Taufkirchen, Germany), fetal bovine serum was from Gibco ...
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bioRxiv - Microbiology 2019Quote: ... filter’s membranes were coated with type IV human placenta collagen (Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
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bioRxiv - Cell Biology 2019Quote: ... Other media components tested were: Human Long R3 IGF1 (Sigma, 91590C), thiazovivin (LC Labs ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in culture medium containing 100ng/ml human IFNγ (Sigma-Aldrich), dispensed into 384-well plates (BD Falcon ...
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bioRxiv - Immunology 2019Quote: ... Samples were then blocked in 10% human serum (Sigma-Aldrich, H4522) in staining buffer on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... and 25 µg/ml type IV collagen from human placenta (Sigma) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... immediately transferred in 250 μl human tubular fluid medium (Millipore, Billerica), punctured with a needle and placed at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 100μg/mL Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Anti-Human IgG −Peroxidase antibody (Fab specific) produced in goat (Sigma) diluted 1:45000 in sample buffer was then added and samples were incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...