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Citations for Millipore Sigma :
1751 - 1800 of 2420 citations for LIMK2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and exposed to blocking solution containing 2% bovine serum albumin or 5% normal goat serum (all from Sigma Aldrich, USA). The fixed cells were incubated overnight at 4 °C with the following primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were blocked for 1 hour with blocking buffer (Cyto-TBS + 2% bovine serum albumin (BSA, Sigma Aldrich, 1002695029)) and incubated with a primary antibody overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilisation was achieved by incubation for 1h in freshly prepared blocking solution (PBS with 5% (v/v) normal donkey serum (D9663, Sigma) and 0.3% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... tissues were treated with a blocking buffer [PBT containing 1% bovine serum albumin (BSA, heat shock fraction; Sigma-Aldrich, A7906)] for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Samples were permeabilized in 0.1% Triton-X-100 for 3 min and then incubated in a blocking solution consisting of 10% FBS (Sigma-Aldrich), 0.01% sodium azide (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were washed in a phosphate buffer saline followed by blocking of the non-specific binding sites with 5% Chemiblocker (Millipore), and 0.2% Triton in phosphate buffer saline ...
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bioRxiv - Bioengineering 2022Quote: ... To improve antibody specificity cells were incubated for 30 min at room temperature in a blocking solution consisting of 0.03% v/v Triton X-100 (Sigma, T8787) and 3% v/v bovine serum albumin (Sigma ...
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bioRxiv - Physiology 2023Quote: ... Samples were blocked for three hours at room temperature in blocking solution [5% normal goat serum (MP Biochemicals) plus 5% bovine serum albumin (Sigma) in PBST] and then incubated overnight at 4°C in primary antibodies diluted in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL antibody (anti-FLAG; Sigma F1804-1MG). 30×10^6 of ESCs crosslinked with formaldehyde as described above were resuspended in 500μL RIPA Buffer (50mM Tris-HCl ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 30 min and exposed to blocking buffer (2 wt% FBS, 0.5 wt% bovine serum albumin (Sigma Aldrich) and 0.1 wt% Tween-20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS (3 x 10 mins) then incubated in a blocking solution comprised of PBS containing 0.5% Triton X-100 (Sigma-Aldrich, Catalogue #X100-500ML ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046, Gapdh; 1:15000, Sigma-Aldrich G8795). Membranes were incubated with the primary antibody solution overnight at 4[ ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 5 minutes at room temperature and the non-specific binding of the antibodies was prevented by applying a blocking solution (PBS supplemented with 5% BSA (Sigma) or 5% goat serum (for Aurora B kinase immunostaining in heart sections ...
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bioRxiv - Molecular Biology 2023Quote: ... a DNA hydrolysis step between the permeabilization and the blocking step was performed by the addition of 2M HCL (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
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bioRxiv - Biophysics 2023Quote: ... Cells were resuspended in 100 µl FACS blocking buffer (phosphate buffered saline (PBS) supplemented with 5% (w/v) bovine serum albumin (BSA) (Sigma)) and centrifuged at 400g for 5 min at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... blocked with SMB plus 1.5 mg/mL Roche Blocking Reagent and 2 mg/mL poly-glutamic acid (1500-5500 Da., Sigma-Aldrich); and then 4 ...
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bioRxiv - Bioengineering 2023Quote: ... To block non-specific binding antibodies, samples were exposed to Blocking Buffer Solution (BBS, composed with 0.5% fetal bovine serum (Sigma-Aldrich), 0.3% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Brain sections were post-fixed with 4% PFA for 10 minutes and then washed with PBS three times and incubated 30 minutes with blocking solution (PBS/0.1% Triton X-100 containing 10% normal goat serum (Sigma-Aldrich)) and then incubation overnight at 4°C in blocking solution with primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated in blocking solution consisting of TBS containing 2% (v/v) normal donkey serum (Catalog # S30–100ML; EMD-Millipore), and 0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed twice with Tris-buffered saline (TBS) and were blocked with blocking buffer (TBS containing 5% donkey serum (Biozol) and 0.25% Triton X-100 (Sigma-Aldrich)) for 1h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then washed 3 times for 20 min in PBS-Triton before incubation with anti-rabbit Alexa Fluor 546 used at 1:500 dilution in blocking buffer containing Hoechst 33342 (Sigma) to label the nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h in a 10% fetal bovine serum (FBS)/PBS blocking buffer containing 0.5% Triton X-100 (cat# X100, Sigma-Aldrich), and incubated overnight at 4°C with the following primary antibodies diluted in the blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... then 50%/50%, then 25%/75%) then incubated in blocking solution (0.3% Triton-X100, 0.45% fish gelatin (Sigma G7765-250ML), 0.6% IgG free BSA (Jackson ImmunoResearch 001-000-162 ...
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bioRxiv - Cell Biology 2024Quote: ... Alfa Aesa) for 10 min and subsequently washed with PBS before blocking with CT (0.5% Triton X-100 (Sigma-Aldrich), 5% ChemiBLOCKER (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies for spinal cord staining were diluted in blocking solution prepared with 5 % Normal Donkey Serum (Millipore #S30-M) and 0.5 % Triton-X in 1 x PBS.
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: Sections were first incubated with a blocking solution made with 5% normal donkey serum and 0.3% TritonX-100 (Sigma-Aldrich) in a PB solution for 1h to prevent the non-specific binding of antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with a blocking solution made with 5% normal goat serum (NGS) and 0.3% TritonX-100 (Sigma-Aldrich) in a PB solution for 1h to prevent the non-specific binding of antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were immersed in blocking solution (5% (vol/vol) horse serum in phosphate buffer with 0.1% (vol/vol) Triton X-100 (Sigma; X100)) for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then rinsed with rinsed with 0.1% Triton X-100 in PBS (0.1%PBST) and then blocked with blocking buffer consisting of 5% normal goat serum (G9023, Sigma-Aldrich) in 0.1% PBST for 1hr at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were then washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% BSA (Sigma), 1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... for 15 min and then cell blocking solution (5% w/v BSA, purchased from Millipore Sigma, dissolved in 1x DPBS) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated for 45 min with the corresponding secondary antibody (Table 3) diluted in blocking buffer plus DAPI (Sigma) in darkness ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma) for 2 h ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: Mounted sections were incubated for 1 hour at room temperature with and blocked using blocking buffer containing (Sigma-Aldrich, #G9023) and 0.1% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue sections were stained with primary antibodies in blocking buffer (PBS + 0.3% Triton-X100 + 1% BSA (cat. # B2518, Sigma-Aldrich)) for 48 hours on a rocker at 4°C ...