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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice and resuspended in RPMI complete media containing 40 U/ml IL-2 (Sigma) and cultured for 2 days ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cancer Biology 2021Quote: ... Nilotinib (AMN107) (Novartis, Basel, Switzerland), and vincristine (Hospira Worldwide Inc., Lake Forest, IL) (Sigma-Aldrich, St. Louis, MO) were dissolved in DMSO and stored at −20°C.
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bioRxiv - Microbiology 2021Quote: ... PBMCs were cultured in 20% FBS-supplemented RPMI media supplemented with 100 ng/mL IL-2 (Sigma, USA) and 50 ng/mL concanavalin A (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-12 (RyD Systems) in the presence of 0.25 μg/mL Brefeldin A and 0.0025 mM Monensin (SIGMA) or in combination with anti-MICa/b ...
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bioRxiv - Developmental Biology 2021Quote: ... At the relevant timepoint embryos were fixed overnight at 4°C using 4 % (w/v) paraformaldehyde (Sigma-Aldrich, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of WT LcαE7 and the Gly137Asp LcαE7 variant was determined by a competition assay between the native-substrate analogue 4-nitrophenol butyrate (4-NPB, Sigma) and the boronic acid compounds ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 4% methanol-free formaldehyde (Thermo, 28906) and permeabilized in .4% Triton X-100 (Sigma, T8787). The Click-iT Cell Reaction Buffer Kit was then used to “click” the biotin alkyne (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the cell culture media was replaced with fresh media and supplemented with 1μM 4-OHT (4-hydroxytamoxifen, Sigma, H7904) for both control and mutant cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cre-mediated recombination was induced in embryos by intraperitoneal injection of 0.5 mg (Cdh5-CreERT2) or 2 mg (Vegfr1-CreERT2, Vegfr3-CreERT2) of 4-hydroxytamoxifen (4-OHT, H7904, Sigma), dissolved in peanut oil (10 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... the mice were given an intraperitoneal injection of 10 mg kg-1 of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) dissolved in a 1:4 mixture of castor oil (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... tadpoles were euthanized using MS-222 (1 g/L) and fixed in 4% paraformaldehyde (Sigma - CAS 30525-89-4) for 3 h at room temperature (RT) ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Cell Biology 2021Quote: β-catenin knock-out in βfl/- cells was induced through the addition of 0.1 μg/mL 4-hydroxy-tamoxifen (4-HT, cat. num. H6278, Sigma) to the media every 24 hours for 72 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age for 20 days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age and maintained on this diet until they were euthanized ...
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bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... and the sample was incubated for 1.5h rotating at 4°C before the addition of 1/4 volume of 3.06% cycloamylose (Sigma-Aldrich). The sample was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and fumaric acid-1,4-13C2,2,3-2H2 (≥99 atom% 13C, ≥98 atom %2H, CAS 100858-52-4, cat. no. 752576) from Sigma Aldrich. All chemicals were used as received.
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bioRxiv - Microbiology 2023Quote: ... subjected to precast 4-12% or 4-20% gels for SDS-PAGE and then transferred to 0.22 μm PVDF membranes (Millipore). After blocking with 5% defatted milk ...
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Topical 4-hydroxytamoxifen (4-OHT; 25 mg/mL or 75 mg/mL in DMSO; 98% Z-isomer, Sigma-Aldrich) was administered to pups on their back at ages P2-P4 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (DM1A, dilution 1:5000) and mouse anti-FLAG M2 antibody (Sigma); Goat Anti-Rabbit IgG(H+L ...
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bioRxiv - Cell Biology 2020Quote: ... 1:1000 anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma Aldrich) or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
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bioRxiv - Cell Biology 2020Quote: Mouse tracheal epithelial cells (mouse AECs) were isolated after pronase (Sigma-Aldrich, 9036-06-0) digestion of WT and Atg16L1HM mouse tracheas and grown on supported membrane inserts (Corning #3460 and #3470 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Anti-mouse BKα mouse monoclonal antibody (L6/60, Millipore; 1 in 1000 Wetsern blot (WB), 1 in 750 in Immunofluorecence (IF)) ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse anti-mouse α–SMA (1:400; clone 1A4; Sigma-Aldrich Chemie Taufkirchen, Germany) was conducted 45 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse anti-Flag M2 and mouse anti-β-actin (AC-15) were from Sigma Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin and mouse anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA); mouse anti-His (CoWin Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Either mouse M2 Flag or mouse HA-7 primary antibodies (Sigma-Aldrich, St. Louis, MO) were used at a 1:1000 dilution in 2.5% BSA in TBST for 1 hour at room temperature ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... mouse monoclonal anti-β actin and mouse monoclonal anti-FLAG (clone M2) were from Sigma; rat monoclonal anti-CD40 (3/23) ...
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bioRxiv - Neuroscience 2023Quote: ... pre-coated with mouse anti-GFP (A11120, Life Technology) or mouse anti-Flag (F3165, Sigma) using Dynabeads Antibody Coupling Kit (14311D ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse, H9658 Sigma-Aldrich, 1:1000) and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-FLAG (Anti-FLAG M2 mouse monoclonal antibody, F1804-1 Sigma-Aldrich, 1:1000), mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:1000 anti-HA mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich), or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: Plasma soluble TREM2 (sTREM2) levels were measured using a Luminex 200 3.1 xPONENT System (EMD Millipore, Chicago, IL, USA) and a custom detection method designed to capture the soluble portion of TREM2 protein as previously described (24) ...
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bioRxiv - Immunology 2019Quote: ... IL-17 and CCL17 were determined with ELISA assays according to the manufacturer’s protocols (Sigma, St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4% PFA-PB; pH 7.4; Sigma-Aldrich, St Louis, MO, USA). Lumbar spinal cords were collected and placed into 4% PFA-PB for 2 hours at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
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bioRxiv - Cancer Biology 2019Quote: ... The endocrine-resistant cells were kept in phenol-red free medium supplemented with 10% heat-inactivated charcoal-stripped-FBS and 1% P/S with the addition of 100 nM 4-hydroxytamoxifen (4-OHT, H7904, Sigma). All cells were kept at 37°C in a humified incubator with 5% CO2.