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Citations for Millipore Sigma :
1751 - 1800 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... injection of human chorionic gonadotropin (5 IU, #CG5; Sigma-Aldrich) 48 h later ...
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bioRxiv - Biochemistry 2020Quote: ... cells were grown with light labeled amino acids with both LPS (1 µg/ml) and curcumin (10 µM, Sigma-Aldrich, St. Louis, MO) and heavy labeled amino acids with LPS (1 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: Odors were purchased at their highest available purity (>97%) and diluted to 1 Pa in light mineral oil (Sigma Aldrich; St. Louis, MO) prior to placing in the flange caps / odor ports and included L-carvone ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mice were orally gavaged at the start of the light cycle (zeitgeber [ZT] 0) with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO) or 0.01 ...
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bioRxiv - Microbiology 2022Quote: ... Anopheles stephensi mosquitoes were reared and maintained at 24°C and 70% humidity under 12-hour light/dark cycles and were fed 0.05% w/v pABA-supplemented 10% (Sigma Aldrich, Cat#100536-250G) w/v sugar water ...
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bioRxiv - Biophysics 2022Quote: ... which was further attenuated by neutral density filters (ND) and converted to circularly polarized light by a quarter-wave plate (WPQ-6328-4M; Sigma Koki, Tokyo, Japan). The collimated and circularly polarized incident beam was focused by a lens (L1 in Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... nitrogen (N) and deuterium (D) isotopes were added: (i) light (L: R0K0) isotopes (12C14N on both Arg and Lys; Sigma-Aldrich: A6969, L8662), (ii ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mice were orally gavaged at the start of the light cycle (zeitgeber [ZT] 0-1) with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO) or 0.01 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Algal cultures were propagated and used in subsequent reinfection experiments and grown at + 22°C under fluorescent light for 16 hour per day in either Basal Medium (Sigma-Aldrich, Milwaukee, WI) or in Modified Bolds 3N Medium (UTEX ...
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bioRxiv - Microbiology 2021Quote: ... were orally gavaged at the beginning of the light cycle (between Zeitgeber time 0-3) with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO) or 0.3 ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting eyecups were then transferred into a light-tight container containing oxygenated (95% O2 and 5% CO2) Ames’ medium (Sigma-Aldrich, Munich, Germany), supplemented with 4 mM D-glucose (Carl Roth) ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was then transferred into a light-tight chamber containing oxygenated (95% O2 and 5% CO2) Ames’ medium (Sigma-Aldrich, Munich, Germany), supplemented with 6 mM D-glucose ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% paraformaldehyde in PBS and immunostained with a FITC-conjugated anti-nucleocapsid monoclonal antibody blend (Light Diagnostics Rabies DFA, EMD Millipore, catalog # 5100) diluted 1:100 in 1% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... Dy-Light Lectin dye at 647 (1:1000) and an anti-smooth muscle actin antibody (α-SMA, 1:500, Millipore, Cy3, mouse monoclonal) were then incubated with tissue for approximately 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Cell Biology 2022Quote: ... 100 U/mL penicillin-streptomycin and the relevant amino acids: arginine-0/lysine-0 (light, 84 and 146 mg/L, Sigma-Aldrich, A6969 and L8662), arginine-6/lysine-8 (heavy ...
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bioRxiv - Neuroscience 2021Quote: ... The eyes were enucleated in dim red light and then transferred to a Petri dish containing oxygenated Ames medium (Sigma-Aldrich, St. Louis MO) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and 7.5 ml of heavy and light protein extracts were prepared and incubated with 40 μl EZview red anti-Myc affinity gel (Sigma-Aldrich, Cat. E6654-1ML) at 4°C for 4 h ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown to exponential phase and treated with UV light using a Stratalinker UV crosslinker or with mitomycin C (Millipore Sigma, St. Louis, MO) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... Under dim red light, the eyes were enucleated and retinas were dissected in carbogenated (95% O2, 5% CO2) Ames’ medium (Sigma-Aldrich or US Biological). Retinas were aligned using the subretinal vasculature(Wei ...
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
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bioRxiv - Biophysics 2019Quote: ... or fibrinogen (0.02 mg/mL, from human plasma, Sigma # F-3879) overnight at 4 °C unless otherwise stated.
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bioRxiv - Systems Biology 2019Quote: ... with standard curves of purified human RBP4 (72) or TTR (Sigma) protein on each blot (57) ...
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bioRxiv - Biochemistry 2019Quote: ... Human serum albumin (catalog # A3782) was from Sigma-Aldrich (Taufkirchen, Germany), fetal bovine serum was from Gibco ...
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bioRxiv - Microbiology 2019Quote: ... filter’s membranes were coated with type IV human placenta collagen (Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...