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Citations for Millipore Sigma :
1751 - 1800 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cytosine b-D-arabinofuranoside (AraC) (Sigma Aldrich, C6645) was added in a 50% medium change (final concentration 2 µM) ...
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bioRxiv - Molecular Biology 2024Quote: ... coated with poly-d-lysine (Sigma-Aldrich, #P7280) and incubated at 37°C and 0% CO2 for 1.5 hours ...
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bioRxiv - Biophysics 2024Quote: ... and embedded in Durcupan resin (Sigma A-D) using standard protocols ...
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bioRxiv - Neuroscience 2024Quote: ... 5 mg/mL D-glucose (Sigma, #G7528-1KG), 0.2 mg/mL L-cystein (MP ...
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bioRxiv - Biochemistry 2024Quote: ... 1-Deoxy-1-morpholino-D-fructose (DMF) (Sigma) as a FN3K substrate in kinase assays.
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bioRxiv - Biochemistry 2024Quote: ... D-lactose (61339) were purchased from Sigma-Aldrich. Alexa Fluor 488 Microscale Protein Labeling Kit (A30006) ...
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bioRxiv - Cell Biology 2024Quote: ... glucose solution [100 mg/ml D-Glucose (Sigma) in ddH2O] ...
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bioRxiv - Cancer Biology 2024Quote: ... 3x with D-PBS + 2% Penicillin-Streptomycin (Sigma), and then rocked for 90 minutes at 4°C with dissociation buffer containing 3mM EDTA (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5 mM D-glucose-13C6 (Millipore Sigma) for the final 2 hours of treatment then scraped or centrifuged and resuspended into 10% trichloroacetic acid ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 μg/mL vitamin D (Sigma-Aldrich). The medium was changed every other day for the duration of the differentiation.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 33 μM D-biotin (Sigma #B4639), 17 μM pantothenate (Sigma #P5155) ...
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bioRxiv - Cell Biology 2024Quote: ... and D-glucose (34mM; High glucose) (Sigma, G5767) were dissolved in growth medium ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Genetics 2024Quote: ... Samples were then incubated overnight at 4°C with a His-tagged mouse monoclonal antibody (cat#70796; Novagen) diluted to 1:1000 in 3% NGS/PAT3 ...
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bioRxiv - Plant Biology 2020Quote: ... cells were harvested by centrifugation and resuspended in 10 mM MES (pH 5.6) buffer containing 10 mM MgCl2 and 200 μM acetosyringone (Sigma, Cat. #D134406) at OD600 = 1.0 ...
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bioRxiv - Plant Biology 2021Quote: Transformed agrobacterium were directly taken from plate with a tip and resuspended into 2 mL of infiltration media (10 mM MES pH 5.7, 10 mM MgCl2, 0.15 mM acetosyringone (Sigma-Aldrich®, #D134406)) by pipetting ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed in Mes buffer (pH 5.5) for 10 min and stained with 100 μM Morin (Sigma-Aldrich, MO, USA) in the same buffer for 1 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pellets were resuspended in infiltration buffer (10 mM MES, 10 mM MgCl2, pH 5.6) with 100 μM acetosyringone (3,5-dimethoxy-4-hydroxyacetophenone, Sigma-Aldrich, Steinheim, Germany) and slightly shaken at room temperature for 1-3 hours ...
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bioRxiv - Genomics 2021Quote: ... and CTCF-AID-eGFP19 mouse embryonic stem (mES) cells were cultured on plates pre-coated with 0.1% gelatin (Sigma-Aldrich, G1393) in Glasgow Modified Essential Medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 150mM K+Glutamate, 50mM HK2PO4, 0.5mM MgCl2, 25mM HEPES-KOH, 25mM MES pH 7.4, 5mM PMSF and 1:200 Millipore Protease Inhibitor Cocktail). Cellular debris and un-lysed cells were clarified by centrifugation at 6000 rpm for 5 min ...
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bioRxiv - Plant Biology 2023Quote: Individual 7-day-old Arabidopsis seedlings were transferred to white 96-well plates containing 200 µl reconstitution buffer (2.5 mM MES pH 5.7 (Sigma-Aldrich, Taufkirchen, Germany), 10 mM CaCl2 (Roth ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM EGTA, 5 mM glucose, 10 mM MES, 5 mM MgCl2, 150 mM NaCl, 1 g/L streptomycin; Sigma Aldrich) at RT and fixed with 4% paraformaldehyde (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 20’000 transduced MEFs were then seeded into one well of a 6 well plate in ‘MES medium’ supplemented with 2ug/ml Doxycycline (D9891-5G, Sigma-Aldrich), 3μM CHIR99021 (4423/50 ...
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bioRxiv - Microbiology 2022Quote: Aldehyde and D-lactate production in Salmonella strains were measured by the Aldehyde Assay Kit and D-Lactate Assay Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... or into microchambers coated with poly-D-lysine (0.1 mg/ml; presynaptic and synaptic compartments) or poly-D-lysine and laminin 10 μg/ml (Sigma; postsynaptic compartment) and cultured at 37°C in a 5% CO2 incubator for 13 days ...