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Citations for Millipore Sigma :
1751 - 1800 of 2589 citations for Apolipoprotein C2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted twice with equal bead volume (200 μl) of AP buffer supplemented with 3XFLAG peptide (Sigma) at 200 μg/ml at room temperature for 15 minutes on a Nutator.
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were dried and reconstituted in 0.1% formic acid and desalted by C18 ziptip (EMD Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of CHLI without transit peptide (1-46 amino acid residues) was cloned into pET32a (Novagen) and pGEX-4T-1 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... IP3R1 was eluted with 500 μM of T433 peptide and concentrated using a 100 kDa Amicon centricon (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich)) was added and incubated with beads overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Systems Biology 2021Quote: ... stringent wash were done with modified lysis buffer (250 mM NaCl + 20 µg/ml peptide FLAG (F3290 Sigma)) prior to elution with 200µg/ml peptide FLAG ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Cell Biology 2021Quote: ... TiO2 beads (5010-21315, GL Sciences) were used to enrich phosphorylated peptide and homemade C8 (66882-U, Sigma) stageTip was used to trap the TiO2 beads for washes ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of each peptide was injected into the abdominal cavity of each mouse along with 0.25 mL Complete Freund’s Adjuvant (Sigma). To enhance the immune response ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP) before elution using wash buffer supplemented with 0.25 mg/mL 3xFLAG peptide (F4799, Sigma-Aldrich). The eluent was then subjected to another round of affinity purification using anti-HA resin (26181 ...
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bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound peptides were eluted in three steps with a solution of 2 mg/ml Oxone (Potassium peroxymonosulfate, Sigma) in H2O ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were extracted using solutions of 50% and 80% acetonitrile (ACN) with 0.5% formic acid (Millipore), and the recovered solution dried down in a vacuum concentrator ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 35 µL of TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) supplemented with 0.5 µg/µL FLAG peptide (Sigma) and incubated at 4 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates containing digested peptides were separated from beads by centrifugation through a 0.45 µM PVDF filter column (Millipore) and cysteines were subsequently reduced and alkylated by addition of 5 mM TCEP and 10 mM CAA for 30 min at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... GB1-TAZ2 binding to MITF peptides was determined utilising a polyhistidine monoclonal mouse antibody (1:5000, Sigma, H1029), incubated on the array for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... high pH reversed-phase peptide prefractionation was performed with sulfonated divinylbenzene (CDS Empore™ SDB-RPS, Sigma-Aldrich) using a step gradient of increasing acetonitrile concentrations (0-60% ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides desalting was carried out using ZipTip U-C 18 spin columns (Cat. No. ZTC18M096, Millipore Corporation U.S.A.). The peptides were dried in a speed vac and then reconstituted with 0.1%Formic acid in water.
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bioRxiv - Biochemistry 2023Quote: ... Core complexes were eluted from anti-Flag resin with Flag buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides contained a C-term PEG-Biotin and were synthesized using a preloaded PEG-Biotin resin (Sigma-Aldrich). Peptides were cleaved from the resin using TFA / Water / Thioanisole / Triisopropylsilane / Phenol / Ethanedithiol – 82.5/0.5/0.5/0.25/0.25/0.25 and precipitated into cold diethyl ether ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT) before elution using wash buffer supplemented with 0.25 mg/ml 3xFLAG peptide (F4799, Sigma-Aldrich). Purified telomerase complex was verified by western blotting.
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bioRxiv - Biophysics 2024Quote: ... The Aβ (1–42) peptide (Stratech, A-1163, 0.5 mg) was initially dissolved in 100% HFIP (Sigma-Aldrich) at a concentration of μM ...
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bioRxiv - Microbiology 2024Quote: ... Stock solutions (1 mg/ml) were prepared by dissolving the peptides in 0.1% trifluoroacetic acid (TFA) (Sigma, Germany). The mass correctness and absence of degradation of the synthetic peptides were verified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was done by adding either 20 μl of 0.2 mg/ml of 3xFLAG peptide (Sigma-Aldrich F4799) or 20 μl of 1x Strep-Tactin Elution Buffer (IBA 2-1042-025 ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 35 µL TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.5 µg/µL FLAG peptide (Sigma) and incubated at room temperature for 15 min at 900 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Cell Biology 2020Quote: ... These slides were rehydrated in 1x PBS at room temperature for 15 min followed by blocking for 1 hour in 1x PBS containing 3% BSA (Bovine serum albumin, Sigma). The slides were then incubated for 1 hour at room temperature with 1xPBS+3% BSA with respective antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos were incubated overnight with an AP-coupled anti-DIG antibody in the blocking solution and finally stained with NBT/BCIP liquid substrate (Sigma). After the staining ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Biophysics 2020Quote: ... The membrane was incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10 % (v/w) dry skim milk (Sigma-Aldrich). The membrane was washed 3 times with PBST for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Rigid paralysis of the skeletal muscles was induced by treatment with the neuromuscular blocking agent decamethonium bromide (DMB) (Sigma Aldrich) (Bowman and Rand ...
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bioRxiv - Plant Biology 2022Quote: ... following an hour of primary antibody incubation in Intercept® (PBS) Blocking Buffer with 0.2% Tween® 20 (Sigma, P1379) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the samples were permeabilized with TritonX-100 at 0.1% in D-PBS with subsequent blocking with 3% Bovine Serum Albumin (Sigma-Aldrich) in D-PBS ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Whole brains were dissected and incubated in blocking solution (5% normal donkey serum and 1% Bovine Serum Albumin (BSA; Sigma) in PBS with 0.1% Triton-X100 (PBS-T) ...