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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Human serum albumin (0.1 µg, Sigma Aldrich) was added to all samples to control the digestion efficiency ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human TMEM119 (1:100, Sigma, #HPA051870), anti-mouse Cd68 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-human β-actin (Sigma, #A5441). Immunoblots were analyzed using horseradish peroxidase secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... along with 5% human AB serum (Sigma) (32–35) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 5% human serum (Sigma-Aldrich) and 5% mouse serum (Sigma-Aldrich)] was added ...
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bioRxiv - Biophysics 2020Quote: ... expressing vimentin with a GFP tag were produced by lentiviral transfection (LentiBrite GFP-Vimentin Lentiviral Biosensor, Merck Millipore) of HeLa cells ...
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cell aliquots were pelleted at 600xg in a swinging bucket rotor centrifuge and washed twice with 200 μL CUT&Tag wash buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine (Sigma); 1x Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... was expressed in COS-7 cells and immobilized to anti-T7 tag-antibody-conjugated agarose beads (EMD Millipore) (Matsui et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Blood levels of TAGs were measured by a colorimetric assay using the Triglyceride determination kit (Sigma-Aldrich, #MAK266). LCN2 plasma levels were determined by ELISA (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Molecular Biology 2021Quote: ... tags and TEV protease were removed by an additional round of affinity chromatography on Ni-NTA-agarose (Sigma). As a final step gel filtration on a Superdex 75 column in 20 mM phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was subjected to Western blot analysis using a polyclonal antibody against the 6Histidine tag (Sigma-Aldrich). After incubation with the second anti-mouse antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: The Cleavage Under Targets and Tagmentation (CUT&Tag) experiments were performed using FLAG antibodies (Flag, Sigma, F1804/F3165) in 3× Flag-POGZ restoring Pogz−/− mESCs by the Yingzi and Frasergen Companies (Wuhan ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... elephas RNAs were reverse transcribed with an oligo dT primer using an enhanced avian myeloblastosis virus reverse transcriptase enzyme (Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore, Burlington, Massachusetts, USA). PM-PDX derived cells were cultured in complete EMEM until a density of approximately 60% was reached ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were infected by incubating the virus for 1h with the cells maintained in DMEM supplemented with 1 μg/ml TPCK-trypsin (Sigma #4370285). The viral input was then removed and cells were kept in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... whole-bodies of virus-injected females (DENV and ZIKV-infected) were homogenised with glass beads in Trizol (Sigma-Aldrich, MA, USA) on a Precellys 24 homogeniser (VWR).
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... P12-ICHIKAWA cells were infected with Lenti-FUW-Luc-mCh-puro virus and selected in liquid culture with puromycin (Sigma-Aldrich) 2.5µg/mL for 7 days following mCherry+ cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...