Labshake search
Citations for Millipore Sigma :
1701 - 1750 of 2451 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti dopamine transporter (DAT; 1:2000; MAB369, EMD Millipore Corporation, Temecula, CA); rabbit anti pmTOR (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with rat polyclonal D1R antibody (1:1000, Sigma-Aldrich, USA) at 4°C overnight ...
-
bioRxiv - Cancer Biology 2021Quote: 96-well plates coated with 50µg/mL Type 1 rat tail collagen (Sigma, UK) were seeded with 7,500 cells per well in medium containing 0.5% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... and rat anti-Gp78/AMFR (1:50, EMD Millipore, St. Louis, MO, Cat #MABC949). The mitochondria were labeled with MitoTracker DeepRed FM according to manufacturer’s instructions (Cat #M22426 ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: Antibodies were used at the following dilutions: rat anti-HA IgG1 (clone 3F10; Sigma) at 1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Rats were intraperitoneally injected with BrdU (150 mg/kg of body weight, Sigma # B5002) once a day for five consecutive days ...
-
bioRxiv - Biophysics 2022Quote: ... The coding sequence for rat Munc18-3 was cloned into pET-15a (Novagen, TX) and codon-optimized for protein expression in bacteria ...
-
bioRxiv - Microbiology 2022Quote: ... followed by 1:500 Alexa Fluor 488 goat anti-rat IgG antibody (Sigma-Aldrich) conjugated antibody ...
-
bioRxiv - Biophysics 2022Quote: ... with the following secondary antibodies: (Fig 3,4) Goat anti-rat CF 568 (Sigma SAb4600086) 1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-platelet derived growth factor receptor alpha (PDGFRa) (1:100, rat, Millipore Cat# CBL1366), anti-GFP (1:2000 ...
-
bioRxiv - Developmental Biology 2022Quote: GH was measured using a mouse/rat growth hormone ELISA kit (Millipore – EZRMGH-45K), based on quantitative sandwich enzyme immunoassay technique ...
-
bioRxiv - Neuroscience 2022Quote: ... Nine-week old rats were injected with DFP (0.5 mg/kg, S.C.; D0879, Sigma) or vehicle (ice-cold PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies were used at the following dilutions: Rat anti-HA IgG1 (clone 3F10; Sigma) at 1:2000 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained for 1 hour at 4 °C with 2% rat serum (Sigma) to reduce antibody binding to Fc receptors ...
-
bioRxiv - Neuroscience 2023Quote: Rats were anesthetised using urethane (1.5 g/kg body weight; Sigma-Aldrich; MA, USA) administered via intraperitoneal injection ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used in this study include rat anti-HA (Sigma; 1:1000 dilution), mouse m2 anti-FLAG (1:1,000 dilution ...
-
bioRxiv - Developmental Biology 2023Quote: Olfactory bulb sections containing rat glomeruli were treated with glycine (50 mM, Sigma-Aldrich) and ammonium chloride (50 mM ...
-
bioRxiv - Genomics 2023Quote: ... The stainings were quenched with the addition of 50 ul Rat Serum (Sigma, R9759) and incubation at 4°C for 10 minutes protected from light ...
-
bioRxiv - Molecular Biology 2023Quote: ... as in28 and rat anti-laminin-2 1:100 233 (Sigma-Aldrich, Cat. #L0663). For macrophage staining these included ...
-
bioRxiv - Biophysics 2023Quote: The rat cardiomyoblast cell-line H9c2 (cells derived from embryonic heart tissue; Sigma Aldrich) was genetically modified using retrovirus to achieve a stable expression of tandem tagged (mCherry-EGFP ...
-
bioRxiv - Biochemistry 2023Quote: ... TR from rat liver (TrxB) was obtained from Sigma-Aldrich (St. Louis, MO, USA). pGEX-4T-1 cells were obtained from GE Healthcare ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with the primary antibody anti-ATE1 (1:200, rat, Sigma-Aldrich), anti- calreticulin (CALR ...
-
bioRxiv - Physiology 2023Quote: The following antibodies were used: rat anti-laminin-α2 (1:1500, Sigma-Aldrich, L0663), mouse anti-BA-D5 (MyHC-I ...
-
bioRxiv - Plant Biology 2024Quote: ... The secondary antibody was rabbit anti-rat IgG conjugated to peroxidase (Sigma-Aldrich A5795), used at a 1:8000 dilution in TBS-T with 2.5% dried milk buffer for one hour ...
-
bioRxiv - Genetics 2023Quote: ... and rat monoclonal anti-laminin-2 (α2 chain; 4H8-2; Sigma Aldrich; 1:500). Secondary antibodies used were goat polyclonal anti-rabbit Alexa Fluor 594-conjugated (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... and rat anti-muscarinic ACh receptor m2 (M2R) antibody (1:500; MAB367, Sigma-Aldrich) at 4 °C for 24 h on a shaker ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were incubated in rat anti-somatostatin (1:250, Millipore cat. No. MAB354, RRID:AB_2255365) at 4°C for 48 hours before the addition of primary antibody rabbit anti-c-Fos (1:3000) ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µL of anti-rat secondary antibody conjugated to horseradish peroxidase (A9037, Sigma-Aldrich) diluted 1:1000 in 5% MPBS were added to each well and incubated for 1.5 hours at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Unilaterally-lesioned rats were injected with D-amphetamine (5.0 mg/kg, i.p.; Sigma-Aldrich) to induce dopamine release from dopaminergic neurons projecting to the striatum and were then placed in a plexiglass cylinder (38 cm diameter × 38 cm height) ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 μg/ml prolactin (mouse recombinant for qPCR, western blot, immunohistochemistry and contraction control; Sigma or Peprotech) or sheep pituitary prolactin for time-lapse and confocal imaging ...
-
bioRxiv - Immunology 2019Quote: ... A subset of mice was treated with IP with recombinant DNase I (20 mg/kg, Sigma-Aldrich) 2 hours after LPS injection24 ...
-
bioRxiv - Microbiology 2021Quote: ... enzymatic activity of recombinant CynD was measured at pH 8.0 using the Ammonia Assay Kit (Sigma-Aldrich). A concentration of 500 nM of CynD was used in all reactions with the following cyanide concentrations ...
-
bioRxiv - Microbiology 2021Quote: ... Digestion of extended E34TSP was done according to the recombinant Enterokinase user protocol TB150 Rev.C 0107(Novagen). The extent of proteolytic activity on our TSP was calibrated via SDS PAGE analysis.
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant MejAgo protein expression and Ni-affinity chromatography purification procedures were performed according to user manual(Novagen). In perticular ...
-
bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
-
bioRxiv - Microbiology 2021Quote: ... Viral titers were calculated from a standard curve generated using recombinant reverse transcriptase (Millipore catalog no. 382129).
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Sf9 cells were transfected with recombinant bacmid DNA using Escort™ IV Transfection Reagent (Sigma, Cat # L3287) in 6 well plates ...
-
bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
-
bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
-
bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...