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Citations for Millipore Sigma :
1701 - 1750 of 2838 citations for OSM Human 227a.a. since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
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bioRxiv - Biochemistry 2023Quote: ... sub-cultured until mid-exponential phase then opsonized in human serum (Sigma-Aldrich, no. H4522) for 30 min ...
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bioRxiv - Immunology 2023Quote: Human macrophages were harvested from culture using PBS+5mM Ethylenediaminetetraacetic acid (EDTA; Sigma Aldrich #E7889) and cell lifters (Fisher Scientific #08-100-240) ...
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bioRxiv - Immunology 2023Quote: ... diluted with a 10x volume of RPMI with 10% heat-inactivated human AB serum (Sigma), and centrifuged at 300 x g for 7 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Monocytes were selected by adhering PBMCs in RPMI with 5% pooled AB human serum (Sigma), 1 mM HEPES (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... A 3 μL drop of 2.5 U/mL human thrombin (Sigma-Aldrich, cat. no. T6884) was added to each well of the platform ...
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bioRxiv - Bioengineering 2024Quote: ... followed by incubation with CF-647 conjugated anti-human IgG (Sigma Aldrich, cat. no. SAB4600181) diluted 1:500 in cell staining buffer for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the adding 50 µg/ml (1:100) of human plasma fibronectin (Sigma-Aldrich) for overnight incubation at 4°C.
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Immunology 2024Quote: ... or goat anti-human secondary HRP antibody (Sigma-Aldrich, #A0170, 1:10,000, for baboon plasma) in 1% BSA in TBS for 1 hour at room 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cell lines and human primary cells were cultured with RPMI 1640 (R8758 Sigma-Aldrich) medium complemented with 10% FBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... filtered and used to infect human myoblasts assisted by polybrene (Sigma-Aldrich, TR-1003-G). When the culture reached 80-90% confluency ...
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bioRxiv - Microbiology 2024Quote: ... Non-specific binding sites on the bacterial cells were blocked using human IgG (Sigma, UK) for an hour and washed with flow buffer again before incubated overnight with the rabbit immune sera (1:1,000 dilution) ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA constructs in a pLKO.1 vector targeting human CHMP5 were purchased from Sigma Aldrich along with 2 control non-targeting shRNAs ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... primary mouse myoblasts and human immortalized myoblasts were transfected with 60nM final of siRNAs (Sigma Aldrich) using LipoRNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC lines were maintained on irradiated MEFs in hESCs medium [DMEM/F12 (Sigma-Aldrich, D6421) supplemented with 20% KO Serum Replacement (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by i.p injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human alveolar type II epithelial cell line A549 was purchased from Sigma-Aldrich (Poole, UK) and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... which is made by supplementing RPMI Medium 1640 with 5 µg/mL human insulin (Sigma-Aldrich), 5 µg/mL human transferrin (Sigma-Aldrich) ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Immunology 2019Quote: ... they were intraperitoneally injected with 2.5 iu human chorionic gonadotropin (HCG, Sigma Aldrich, St. Louis, MO) and immediately mated with male mice overnight (16 hours) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on glass coverslips coated with 50µg/mL human type IV collagen (Sigma C7521) 24 to 48h before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... META-enriched cultures were isolated via resistance to complement-mediated lysis in 20% human serum (Sigma) for 30min at 37°C and validated via sherp expression [21,23].
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bioRxiv - Immunology 2019Quote: Human and mouse omentum samples were fixed for one hour on ice in 10% NBF (Sigma) and then permeabilized in PBS 1% Triton-X 100 (Sigma ...
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bioRxiv - Genomics 2021Quote: DNA from human keratinocyte cells was extracted using the “GenElute-Mammalian Genomic DNA Miniprep Kit” (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then 50 μL of alkaline phosphatase-conjugated secondary goat anti-Human anti-IgG (Sigma Cat # A9544), anti-IgA (Abcam Cat # AB97212) ...
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bioRxiv - Bioengineering 2021Quote: Immortalized human neural progenitor cells derived from the ventral midbrain (ReNCell VM) were obtained from Millipore. Cells were expanded on laminin-coated tissue culture flasks ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (A1653) and mouse serum albumin (A3139) were purchased from Sigma (St Louis, MO). Fluorescently labeled human serum albumin (HS1-S5-1 ...
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bioRxiv - Neuroscience 2020Quote: ... were co-transfected into U87 human primary glioblastma cells by using ESCORT V transfection reagent (Sigma). The fold increase in relative luciferase activity is a product of the luciferase activity induced by TCF7l2-S2 or TCF7l2-E2 (backbone vector pCMV ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
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bioRxiv - Cell Biology 2021Quote: ... HAP1 cells were transfected with 600 ng/ml human VRK2 MISSION® esiRNA (EHU070731, Sigma-Aldrich) using Lipofectamine RNAiMAX (13778075 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Cell Biology 2021Quote: ... Fibronectin-coated coverslips were prepared by first covering them with 100 µL of human (Sigma F0556) or bovine (Sigma F01141 ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
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bioRxiv - Systems Biology 2021Quote: Dispersed human islets and iPSC-beta cells were exposed to 0.5 mmol/L palmitate (Sigma-Aldrich) for 24 h and 48h ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
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bioRxiv - Microbiology 2021Quote: ... ChIP MIC in serum was determined in the presence of 50% (heat inactivated) human serum (Sigma) and 50% MHB-II ...
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bioRxiv - Microbiology 2019Quote: ... The immortalized human cerebral microvascular endothelial cell line hCMEC/D3 (59) was purchased from Millipore Sigma and maintained in Endothelial Cell Growth Basal Medium-2 (EBM2 ...
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bioRxiv - Cell Biology 2020Quote: ... DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen) to a retroviral vector compatible plasmid (PPRIG ...
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bioRxiv - Cell Biology 2021Quote: ... FN coating was accomplished by adding phosphate buffered saline (PBS, Cellgro) and human plasma fibronectin (Millipore) to six-well plates (Corning) ...
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bioRxiv - Biochemistry 2022Quote: ... Type O+) at 4% haematocrit in NaHCO3 and AlbumaxII/human serum supplemented RPMI (RPMI (Sigma Aldrich), 25 mM HEPES (GIBCO) ...