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Citations for Millipore Sigma :
1701 - 1750 of 3694 citations for HMGB2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
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bioRxiv - Biophysics 2019Quote: ... or fibrinogen (0.02 mg/mL, from human plasma, Sigma # F-3879) overnight at 4 °C unless otherwise stated.
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bioRxiv - Systems Biology 2019Quote: ... with standard curves of purified human RBP4 (72) or TTR (Sigma) protein on each blot (57) ...
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bioRxiv - Biochemistry 2019Quote: ... Human serum albumin (catalog # A3782) was from Sigma-Aldrich (Taufkirchen, Germany), fetal bovine serum was from Gibco ...
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bioRxiv - Microbiology 2019Quote: ... filter’s membranes were coated with type IV human placenta collagen (Sigma) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
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bioRxiv - Cell Biology 2019Quote: ... Other media components tested were: Human Long R3 IGF1 (Sigma, 91590C), thiazovivin (LC Labs ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in culture medium containing 100ng/ml human IFNγ (Sigma-Aldrich), dispensed into 384-well plates (BD Falcon ...
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bioRxiv - Immunology 2019Quote: ... Samples were then blocked in 10% human serum (Sigma-Aldrich, H4522) in staining buffer on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... and 25 µg/ml type IV collagen from human placenta (Sigma) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... immediately transferred in 250 μl human tubular fluid medium (Millipore, Billerica), punctured with a needle and placed at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 100μg/mL Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Anti-Human IgG −Peroxidase antibody (Fab specific) produced in goat (Sigma) diluted 1:45000 in sample buffer was then added and samples were incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: ... coated with 50 μg/mL collagen from human placenta (Sigma Aldrich) at ~10,000 cells/cm2 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), 1 μM metribolone (Toronto Research Chemicals ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Biochemistry 2020Quote: PDP-23 was mixed with human pooled male serum (Sigma-Aldrich) to a final concentration of 50 µg/mL and incubated at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: In human HEK CLASH beads conjugated with anti-Flag M2 (Sigma), in FLASH Protein A agarose conjugated with IgG and anti-FBL AB were used ...
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bioRxiv - Microbiology 2020Quote: ... human serum and endothelial growth supplement were from Sigma (Taufkirchen, Germany).
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bioRxiv - Microbiology 2021Quote: ... Cells were then stained with anti-human-IgG-FITC (Sigma, F9512) or anti-mouse-IgG-FITC (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL human plasma fibronectin (Corning ...
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bioRxiv - Microbiology 2020Quote: ... Results were normalized to ng actin using Human DNA standard (Sigma). Primers used for quantification of actin were ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were concentrated using Amicon Ultra-4 5 kDa (Millipore). Antibody concentration was determined by absorbance measurement at 280 nm using a Nanodrop and purity was determined using SDS-PAGE ...