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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Ceramide Synthase 3 CERS3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Primary antibodies: Sept2 (SIGMA Atlas Antibodies HPA018481), GFP (Roche 12600500) ...
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bioRxiv - Microbiology 2019Quote: ... antibody and rabbit anti-Flag antibody (Sigma) solution for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Neuroscience 2019Quote: ... samples were washed 3 × 3 minute with PBS followed by a second 30-minute fixation step in 1% osmium tetroxide (Sigma-Aldrich, 75632). A final 3 × 3 minute PBS wash step was performed before samples were serially dehydrated in ethanol with a final 30-minute dehydration step using hexamethyldisilazane (HMDS ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Neuroscience 2020Quote: ... FBS supplementation was decreased to 3% FBS 3 days prior to the addition of 10□M Retinoic Acid (RA; Sigma-Aldrich, R2625). During differentiation ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 (15 μg/mL) was immobilized on AR2G biosensors activated by 1-ethyl-3-[3-dimethylaminopropy] carbodiimide hydrochloride (EDC, E1769, Sigma, Shanghai, CHN) and sulfo-N-hydroxysulfosuccinimide (s-NHS ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hrs followed by addition of 3 µM Asunaprevir (Apexio: BMS-650032) and 500 µM 3-Indoleacetic acid (Sigma Aldrich: I2886). Cells were treated with following inhibitors to inhibit indicated kinases ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Immunology 2024Quote: ... The extract was reconstituted in a 500-μl solution of methanol/water (1:1) and filtered through a 3-kDa molecular filter (Amicon® Ultra Centrifugal Filter, 3 kDa MWCO, Millipore Sigma) at 4°C for 90 minutes to remove proteins ...
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bioRxiv - Biochemistry 2024Quote: ... 8029.3), and 0.5% sodium deoxycholate (AppliChem, A1531) in PBS supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726, P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... 20 μL/mL Phosphatase Inhibitor Cocktail 3 (Sigma, P0044), 20 μL/mL Phosphatase Inhibitor Cocktail 2 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, #I7018) was supplemented to the media for the indicated time ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% of phosphatase inhibitor cocktail 3 (Sigma-Aldrich) was added to the adherent cells and permitted to incubate on ice for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), for 1 hour at 4°C on rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of 8 mg/ml hexadimethrine bromide (Sigma) was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-γ-adaptin (100/3) from Sigma. Hybridomas producing antibodies against LDL receptor (C7 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in 3% H2O2 (Sigma-Aldrich, Shanghai, China) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine, Sigma) or a combination of all three (BCKAs) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pretreated with 3 μM FTY720 (Sigma-Aldrich) for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... with 3 % bovine serum albumin (BSA, Sigma-Aldrich # A7906) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... ICC blocking solution (3% w/v BSA (Sigma-Aldrich), 3% v/v goat serum (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... and Phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), and incubated 10 min on ice ...
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bioRxiv - Immunology 2021Quote: ... in the presence of Monensin (3 µM, Sigma Aldrich) for 4 h ...
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bioRxiv - Genetics 2021Quote: Cell fixation with 3% formaldehyde (Sigma-Aldrich, Cat. F8775) was performed as described in Dauban et al ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1 mM 3-Isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich), 0.1 mM 8-Bromoadenosine 30,50-cyclic monophosphate sodium salt (cAMP ...
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bioRxiv - Cancer Biology 2020Quote: ... and active caspase-3 (1:1 000, Millipore, AB3623). Three fields of view for each slide were imaged using an Olympus BX43 Upright Microscope and cells were manually counted and scored using ImageJ Software.
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bioRxiv - Plant Biology 2022Quote: Indole-3-acetic acid (IAA, 10 μM; Sigma-Aldrich) and (+/-)-abscisic acid (ABA ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), and sonicated using a fine probe (0.5-sec pulse at an amplitude of 20% ...
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bioRxiv - Neuroscience 2021Quote: ... 3’-diaminobenzadine substrate solution (0.5mg/ml; D5637, Sigma Aldrich) in 0.01% H2O2 in PBS was determined for each antigen ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in 0.9% sterile NaCl solution (saline ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in PBS 1X was injected into the mouse left hind paw using a Hamilton syringe ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal anti-GluR2/3 (1:200, #AB1506, Millipore), mouse monoclonal anti-calretinin (1:750 ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich) and sonicated using a fine probe (0.5-sec pulse at amplitude of 20% ...