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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml DOX (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce TCF7L1 re-expression (DOX (+)) ...
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bioRxiv - Bioengineering 2020Quote: ... One hemisphere of the brain (cut into coronal blocks) and the liver were flash frozen in 2-methylbutane (Sigma Aldrich, M32631) chilled with dry ice ...
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bioRxiv - Biophysics 2021Quote: ... the DNA construct was attached to streptavidin coated beads by incubating 0.5 µl of picomolar DNA stock solution and 2 µl My- One beads in 250 µl 1x PBS (Sigma-Aldrich, USA) for 5 min and subsequently introduced in the flow cell for 5 min to allow formation of digoxigenin-anti-digoxigenin bonds ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were randomized in two groups of which one henceforth was treated with 2 mg/ml BelaDox (Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo knockdown (Dox (+)) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml Dox (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce sh4E-BP1_2 expression ...
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bioRxiv - Developmental Biology 2023Quote: 40 testes were dissected from one-day old adult heph-GFP males and digested in 200 µL of 2 mg/mL collagenase (Sigma C9407) in HEPESCA solution at 37° for one hour ...
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bioRxiv - Physiology 2019Quote: ... 8 bromo-cGMP (cGMP)37 (Sigma-Aldrich, Oakville, ON, Canada). Unstimulated controls consisted of tubules bathed in physiological saline with no diuretic application ...
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bioRxiv - Molecular Biology 2021Quote: ... and 26 mM NaHCO3 and 98 µM bromo-deoxyuridine (Sigma), and pre-plated for 1.5 hours at 37 °C to obtain a cardiomyocyte-rich fraction ...
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bioRxiv - Microbiology 2020Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected and glycogen was added as a carrier ...
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bioRxiv - Microbiology 2021Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected ...
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bioRxiv - Microbiology 2020Quote: ... samples in TRIzol were separated using Bromo-chloro-propane (Sigma). The aqueous phase was collected and glycogen was added as a carrier ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Sigma). At 72 h p.t. ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μg/ml N-tosyl-l-phenylalanine chloromethyl ketone (TPCK) treated trypsin (Sigma Aldrich) for MDCK cells ...
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bioRxiv - Microbiology 2022Quote: ... overlaid with serum-free DMEM containing1g/ml tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Sigma-Aldrich), and incubated at 37°C ...
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bioRxiv - Genomics 2023Quote: ... The supernatants were transferred to fresh tubes and 100 µL of BCP (1-bromo-3-chloropropane, Sigma) were added ...
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bioRxiv - Developmental Biology 2021Quote: ... 7-AAD (Sigma, 10 µg ml−1, 1:100) or Zombie Aqua (Biolegend ...
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bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... mice were anesthetized by intraperitoneal injection with Avertin (2, 2, 2-Tribromoethanol, Sigma-Aldrich, St. Louis, MO, USA; catalog #T4). Cardiac perfusion was performed with 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
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bioRxiv - Immunology 2023Quote: ... T cells transduced with the anti-hCD19 CAR construct 42 were cultivated for additional 2 days in presence or without 2 mM 2-DG (Sigma) before adoptive transfer into MC38-hCD19 tumor-bearing mice.
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bioRxiv - Developmental Biology 2023Quote: ... The following factors were applied to experimental BPs in culture at the specified concentrations: 2-Deoxyglucose (2-DOG) 2 mM (SIGMA), Sodium Pyruvate (NaP ...
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...
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bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
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bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
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bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
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bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
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bioRxiv - Immunology 2019Quote: ... 2’-Deoxyadenosine (dAdo) and 2’-Deoxycytidine (dCyt) were purchased from Sigma-Aldrich Japan (Tokyo ...
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bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).
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bioRxiv - Immunology 2020Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM 9-Amino-6-Chloro-2-Methoxyacridine (ACMA, Sigma-Aldrich) to establish a K+ gradient ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µM epoxomicin and 50 µM (Z-LL)2-ketone (Merck Millipore) were added from stock solutions in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1µM Oligomycin A and 50mM of 2-deoxyglucose (2-DG) (Sigma, D3179). For mitostress tests ...
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bioRxiv - Pathology 2022Quote: ... in PBS, 2x) and soaked in 12.5% (2h; 4°C) and 25% (7 days, 4°C) sucrose (Sigma). Kidneys were embedded in OCT (Tissue-Tek® ...
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bioRxiv - Immunology 2023Quote: ... Overnight incubation with mouse anti-HLA class I-ABC and 4h incubation with goat anti-Mouse Alexa Fluor 488 (Supplementary Table S5) and DAPI (1 mg/mL, Sigma-Aldrich) were performed in 10% FCS and 0.2% Triton X-100 in PBS ...