Labshake search
Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... or 10 μM 2-cis,4-trans-Abscisic acid (ABA) (Sigma-Aldrich), respectively ...
-
bioRxiv - Cell Biology 2023Quote: ... salivary glands were fixed for 2 hours in 4% PFA (Paraformaldehyde, Sigma) at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100U of penicillin/streptomycin and 10-4 M 2-Mercaptoethanol (M7522, Sigma) under hypoxic condition (2% O2 ...
-
bioRxiv - Bioengineering 2023Quote: ... 4 breaths with 2% Sodium Deoxycholate (SDC) (Cat. # D6750, Sigma Aldrich, UK), 8 breaths with PBS supplemented with 1% penicillin/streptomycin solution (PBS - P/S) ...
-
bioRxiv - Biochemistry 2023Quote: ... dialyzed three times against 4 L of 2 mM β-mercaptoethanol (Sigma) in water ...
-
bioRxiv - Immunology 2023Quote: ... Cells were fixed by adding 2 mL 4 % paraformaldehyde (Sigma Aldrich #F8775) for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... or 2-mercaptobenzothiazole (MBT – Cat No. #149-30-4, Sigma-Aldrich, USA) as the matrix in two different protocols.
-
bioRxiv - Immunology 2023Quote: ... Cells were fixed by adding 2 mL 4 % paraformaldehyde (Sigma Aldrich #F8775) for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... Treatment with HU (Sigma-Aldrich, 0, 0.5, 1, 2, 4, 8 mM) was for 24h ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887). These cells were chosen as an experimental system as previously described (Massacci et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... experiments were conducted in the presence of 10μM DNQX [6,7-Dinitroquinoxaline-2,3 (1H,4H-dione)] (Sigma, Cat#D0540)] and 50μM APV [DL-2-Amino-5-phosphonopentanoic acid] (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... 6×104 HPMEC or 2×105 Calu-3 cells were seeded on 0.2% gelatin-(Sigma) coated glass coverslips in 24-well plates ...
-
bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
-
bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
-
bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
-
bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
-
bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
-
bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
-
bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
-
bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
-
bioRxiv - Immunology 2019Quote: ... 24 and 48 hours and then fixed with 4°C methanol for 3 minutes and stained with safranin (Sigma-Aldrich, Gilligham, UK) for 10 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
-
bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
-
bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were then rinsed 3 times with PBS and mounted onto microscope slides with 5 μl Mowiol 4-88 (Sigma-Aldrich #81381). Cells were visualized and imaged with a Nikon Ti-Eclipse A1M Confocal Microscope using a 60X objective ...
-
bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
-
bioRxiv - Plant Biology 2021Quote: ... lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) were purchased from Sigma-Aldrich. Vismione H and madagascine were obtained from PGE2 fraction of Psorospermum glaberimum as previously described (Gallé ...
-
bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
-
PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Expression of constructs was suppressed by addition of 0.5 µM and indole-3-acetic acid (IAA; Sigma, I5148). 2i F1 hybrid 129/Sv:Cast/Eij mESCs cells were cultured for 2 weeks on primary mEFs in 2i ES cell culture media ...
-
bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... we replaced media with either 400 uM mimosine + 500 uM indole-3-acetic acid (auxin; Sigma-Aldrich I5148) supplemented media to degrade RAD21 or 400 uM mimosine-supplemented media alone as an untreated control ...
-
bioRxiv - Genomics 2020Quote: ... SCC1 degradation was induced by addition of 500 µM auxin (indole-3-acetic acid sodium salt, Sigma-Aldrich). Newly synthesized RNA was labelled by addition of 4-Thiouridine (4SU ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4% PFA fixed zebrafish embryos were embedded in 6% low-melting agarose (LMA, Sigma-Aldrich) dissolved in 0.1% Tween-20 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449, Millipore), 5ug H2AK119ub (8240 ...
-
bioRxiv - Microbiology 2020Quote: ... 4 mM stable glutamine without sodium pyruvate (Biochrom) and supplemented with 6% iFCS (Sigma-Aldrich). Primary human macrophages were cultured in RPMI medium containing 5.5 g × l-1 NaCl ...
-
bioRxiv - Cancer Biology 2021Quote: ... along with a photo-initiator 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure2959-410896, Sigma-Aldrich, USA), at a concentration of 0.5 mg/mL followed by exposure to UV-365 for 10 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was incubated for 1h at 4 °C in the presence or absence of 20 or 100 µM FTY720 (Sigma-Aldrich) or fumonisin B1 (Sigma-Aldrich) ...