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Citations for Millipore Sigma :
1651 - 1700 of 2445 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-phospho-Histone H3 (pSer28) (clone HTA28) (Sigma Cat#H9908, RRID: AB_260096), mouse monoclonal anti-Human Nuclear Antigen (clone 235-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... or the Anti-HA Affinity Matrix from rat immunoglobulin G (IgG)1 (Sigma-Aldrich) for 2 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... and the e-cadherin with monoclonal rat anti-e-cadherin (Sigma, catalogue no. U3254) primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma ...
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... XF24 cell culture microplates were coated with Rat Tail Collagen I (Sigma Cat#C3867) before plating wells with prepared SVF as described previously ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a secondary anti-Rat IgG peroxidase-conjugated antibody (Sigma-Aldrich), diluted 1:5000 in 5% skim milk ...
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bioRxiv - Microbiology 2021Quote: ... Insulin was measured using the rat/mouse insulin ELISA kit from Millipore (EZRMI-13K), according to the manufacturer’s instructions (Wang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 0.25% trypsin-EDTA solution and rat tail collagen I were obtained from Sigma-Aldrich. Polybrene ...
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bioRxiv - Neuroscience 2021Quote: ... the rat dams were given access to saccharin (Sigma Life Sciences, St. Louis, Missouri) water containing either 0% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The rats were perfused first with pre-chilled Dulbecco’s phosphate-buffered saline (DPBS; Sigma), and then with 4% PFA (bought as 20% PFA from Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: TNTPrL-P.ChR2:hM4Di rats were terminally anaesthetised with urethane (1.2-2g/kg i.p, Sigma). The spinal cord was exposed by laminectomy over T13-L3 spinal segments to allow access to the spinal cord (Leith et al. ...
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bioRxiv - Neuroscience 2020Quote: ... D2-ChR2 rats (n = 4) were anaesthetized with urethane (1.44 g kg−1, Sigma). The total dose was administered in three separate intraperitoneal injections ...
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bioRxiv - Microbiology 2021Quote: Anti-HA (3F10) rat antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-CD63 (Ts63 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Rats were administered a sub-cutaneous (s.c.) injection of ketoprofen (Anafen®; Sigma Aldrich, Oakville ON ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1/7000 dilution of anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich) as secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1/7000 dilution of anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich). Color was developed using BCIP/NBT (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... rats received an intraperitoneal (IP) injection of desipramine hydrochloride (20 mg/kg, Sigma-Aldrich). For the surgery ...
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bioRxiv - Neuroscience 2020Quote: ... The amounts injected were as follows: 0.1 mg/kg rat α-CGRP (Sigma-Aldrich), 0.3 mg/kg PACAP-38 (Bachem) ...
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bioRxiv - Neuroscience 2021Quote: ... rats were first injected with 0.5 μl control enzyme (penicillinase, P0389-1KU, Sigma, USA) or chABC (C3667-10UN ...
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bioRxiv - Physiology 2020Quote: Insulin concentrations were measured by ELISA (EZRMI-13K Rat/Mouse insulin ELISA, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore), in EndoGro-MV medium (Merck Millipore ...
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bioRxiv - Immunology 2020Quote: 3D Collagen type I was prepared using 8 parts rat collagen type I (Sigma) and 1 part 10x DMEM and was neutralized to pH 7.0-7.5 by adding 0.5 parts 0.5N NaOH ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used: rat anti-a-tubulin (MAB1864, Millipore, 1:10000), mouse anti-Aurora-A (clone 5C3 ...
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bioRxiv - Physiology 2020Quote: ... anti-rat or anti-guinea pig secondary antibodies (1:500; Sigma Aldrich, MO, USA) in combination with DAPI (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... healthy rat hearts were embedded and frozen in OCT medium (Sigma-Aldrich, Gillingham, UK) and cut into 5μm sections ...
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bioRxiv - Neuroscience 2019Quote: ... rats were injected with lithium-chloride (127 mg/kg, IP, Sigma, St. Louis, MO) 24 hours prior to pilocarpine hydrochloride (50 mg/kg ...
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bioRxiv - Neuroscience 2020Quote: ... the rat dams were given access to saccharin (Sigma Life Sciences, St. Louis, Missouri) water containing either 0% (v/v ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... For Benzidine staining of naked mole-rat methylcellulose assays a 0.2% benzidine dihydrochloride (Sigma) solution in 0.5M acetic acid was prepared ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in 1:100 rat anti-SST (Millipore), 1:1000 mouse anti-PV (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used in this study include rat anti-HA (Sigma; 1:500 dilution), mouse m2 anti-FLAG (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti dopamine transporter (DAT; 1:2000; MAB369, EMD Millipore Corporation, Temecula, CA); rabbit anti pmTOR (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with rat polyclonal D1R antibody (1:1000, Sigma-Aldrich, USA) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: 96-well plates coated with 50µg/mL Type 1 rat tail collagen (Sigma, UK) were seeded with 7,500 cells per well in medium containing 0.5% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and rat anti-Gp78/AMFR (1:50, EMD Millipore, St. Louis, MO, Cat #MABC949). The mitochondria were labeled with MitoTracker DeepRed FM according to manufacturer’s instructions (Cat #M22426 ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Antibodies were used at the following dilutions: rat anti-HA IgG1 (clone 3F10; Sigma) at 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were intraperitoneally injected with BrdU (150 mg/kg of body weight, Sigma # B5002) once a day for five consecutive days ...